Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion
Ischemia reperfusion injury (IRI) is a critical problem in liver transplantation that can lead to life-threatening complications and substantially limit the utilization of livers for transplantation. However, because there are no early diagnostics available, fulminant injury may only become evident...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550443/?tool=EBI |
| _version_ | 1818841130358276096 |
|---|---|
| author | Reinier J. de Vries Stephanie E. J. Cronin Padraic Romfh Casie A. Pendexter Rohil Jain Benjamin T. Wilks Siavash Raigani Thomas M. van Gulik Peili Chen Heidi Yeh Korkut Uygun Shannon N. Tessier |
| author_facet | Reinier J. de Vries Stephanie E. J. Cronin Padraic Romfh Casie A. Pendexter Rohil Jain Benjamin T. Wilks Siavash Raigani Thomas M. van Gulik Peili Chen Heidi Yeh Korkut Uygun Shannon N. Tessier |
| author_sort | Reinier J. de Vries |
| collection | DOAJ |
| description | Ischemia reperfusion injury (IRI) is a critical problem in liver transplantation that can lead to life-threatening complications and substantially limit the utilization of livers for transplantation. However, because there are no early diagnostics available, fulminant injury may only become evident post-transplant. Mitochondria play a central role in IRI and are an ideal diagnostic target. During ischemia, changes in the mitochondrial redox state form the first link in the chain of events that lead to IRI. In this study we used resonance Raman spectroscopy to provide a rapid, non-invasive, and label-free diagnostic for quantification of the hepatic mitochondrial redox status. We show this diagnostic can be used to significantly distinguish transplantable versus non-transplantable ischemically injured rat livers during oxygenated machine perfusion and demonstrate spatial differences in the response of mitochondrial redox to ischemia reperfusion. This novel diagnostic may be used in the future to predict the viability of human livers for transplantation and as a tool to better understand the mechanisms of hepatic IRI. |
| first_indexed | 2024-12-19T04:21:11Z |
| format | Article |
| id | doaj.art-8d96b9d2958643e1949f6fefe072eaea |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T04:21:11Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8d96b9d2958643e1949f6fefe072eaea2022-12-21T20:36:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-011610Non-invasive quantification of the mitochondrial redox state in livers during machine perfusionReinier J. de VriesStephanie E. J. CroninPadraic RomfhCasie A. PendexterRohil JainBenjamin T. WilksSiavash RaiganiThomas M. van GulikPeili ChenHeidi YehKorkut UygunShannon N. TessierIschemia reperfusion injury (IRI) is a critical problem in liver transplantation that can lead to life-threatening complications and substantially limit the utilization of livers for transplantation. However, because there are no early diagnostics available, fulminant injury may only become evident post-transplant. Mitochondria play a central role in IRI and are an ideal diagnostic target. During ischemia, changes in the mitochondrial redox state form the first link in the chain of events that lead to IRI. In this study we used resonance Raman spectroscopy to provide a rapid, non-invasive, and label-free diagnostic for quantification of the hepatic mitochondrial redox status. We show this diagnostic can be used to significantly distinguish transplantable versus non-transplantable ischemically injured rat livers during oxygenated machine perfusion and demonstrate spatial differences in the response of mitochondrial redox to ischemia reperfusion. This novel diagnostic may be used in the future to predict the viability of human livers for transplantation and as a tool to better understand the mechanisms of hepatic IRI.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550443/?tool=EBI |
| spellingShingle | Reinier J. de Vries Stephanie E. J. Cronin Padraic Romfh Casie A. Pendexter Rohil Jain Benjamin T. Wilks Siavash Raigani Thomas M. van Gulik Peili Chen Heidi Yeh Korkut Uygun Shannon N. Tessier Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion PLoS ONE |
| title | Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion |
| title_full | Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion |
| title_fullStr | Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion |
| title_full_unstemmed | Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion |
| title_short | Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion |
| title_sort | non invasive quantification of the mitochondrial redox state in livers during machine perfusion |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550443/?tool=EBI |
| work_keys_str_mv | AT reinierjdevries noninvasivequantificationofthemitochondrialredoxstateinliversduringmachineperfusion AT stephanieejcronin noninvasivequantificationofthemitochondrialredoxstateinliversduringmachineperfusion AT padraicromfh noninvasivequantificationofthemitochondrialredoxstateinliversduringmachineperfusion AT casieapendexter noninvasivequantificationofthemitochondrialredoxstateinliversduringmachineperfusion AT rohiljain noninvasivequantificationofthemitochondrialredoxstateinliversduringmachineperfusion AT benjamintwilks noninvasivequantificationofthemitochondrialredoxstateinliversduringmachineperfusion AT siavashraigani noninvasivequantificationofthemitochondrialredoxstateinliversduringmachineperfusion AT thomasmvangulik noninvasivequantificationofthemitochondrialredoxstateinliversduringmachineperfusion AT peilichen noninvasivequantificationofthemitochondrialredoxstateinliversduringmachineperfusion AT heidiyeh noninvasivequantificationofthemitochondrialredoxstateinliversduringmachineperfusion AT korkutuygun noninvasivequantificationofthemitochondrialredoxstateinliversduringmachineperfusion AT shannonntessier noninvasivequantificationofthemitochondrialredoxstateinliversduringmachineperfusion |