Evaluation of native Trichoderma isolates for the management of sugarcane smut (Ustilago scitaminea) in sugar plantations of Ethiopia
Biological control of sugarcane smut (Ustilago scitaminea Syd.) using Trichoderma isolates is one of the untapped potentials in Ethiopian sugarcane plantations. To this end, this study was carried out with the objective to identify potential native Trichoderma isolates for the management of sugarcan...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Cogent Food & Agriculture |
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| Online Access: | http://dx.doi.org/10.1080/23311932.2021.1872853 |
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| author | Samuel Tegene Mashilla Dejene Habtamu Terefe Girma Tegegn Esayas Tena Amare Ayalew |
| author_facet | Samuel Tegene Mashilla Dejene Habtamu Terefe Girma Tegegn Esayas Tena Amare Ayalew |
| author_sort | Samuel Tegene |
| collection | DOAJ |
| description | Biological control of sugarcane smut (Ustilago scitaminea Syd.) using Trichoderma isolates is one of the untapped potentials in Ethiopian sugarcane plantations. To this end, this study was carried out with the objective to identify potential native Trichoderma isolates for the management of sugarcane smut. A total of 120 soil samples were collected from Kesem, Metehara, and Wonji sugarcane plantation fields. Consequently, 21 Trichoderma isolates were recovered from the samples, and in-vitro antagonist study of Trichoderma isolates against U. scitaminea was done using dual culture technique on fresh PDA. Among the native isolates, 12 of them revealed a pathogen suppression potential of >90% at 7 days after inoculation and most of them had higher pathogen suppression than the commercial isolate. However, five of the isolates were selected as potential candidates based on distinct suppression features, growth rate, and antagonistic performance. Identification of the five effective Trichoderma isolates (WI4-27, MKI2-k17, MI3-m33, WI12-25, and MKI3) was done based on macroscopic and microscopic characteristics. Thus, the isolates were identified as T. harzianum, T. asperellum, T. viride, T. viride, and T. harzianum, respectively. Accordingly, these isolates could be used as one component of integrated smut management practices once a study on compatibility with fungicides, in-vivo efficacy test against smut, and substrate selection for mass production of the isolates were commenced. |
| first_indexed | 2024-12-20T14:00:35Z |
| format | Article |
| id | doaj.art-8d9a539b58b544a7b965a9a112681e89 |
| institution | Directory Open Access Journal |
| issn | 2331-1932 |
| language | English |
| last_indexed | 2024-12-20T14:00:35Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cogent Food & Agriculture |
| spelling | doaj.art-8d9a539b58b544a7b965a9a112681e892022-12-21T19:38:23ZengTaylor & Francis GroupCogent Food & Agriculture2331-19322021-01-017110.1080/23311932.2021.18728531872853Evaluation of native Trichoderma isolates for the management of sugarcane smut (Ustilago scitaminea) in sugar plantations of EthiopiaSamuel Tegene0Mashilla Dejene1Habtamu Terefe2Girma Tegegn3Esayas Tena4Amare Ayalew5Ethiopian Sugar Corporation, Research CenterSchool of Plant Sciences, Haramaya UniversitySchool of Plant Sciences, Haramaya UniversityMelkasa Agricultural Research CenterSugarcane Variety Improvement Research Program, Ethiopian Sugar Corporation, P.O. Box 15, Wonji, EthiopiaPartnership for Aflatoxin Control in Africa (PACA), Africa Union, P.O. Box 3243, Addis Ababa, EthiopiaBiological control of sugarcane smut (Ustilago scitaminea Syd.) using Trichoderma isolates is one of the untapped potentials in Ethiopian sugarcane plantations. To this end, this study was carried out with the objective to identify potential native Trichoderma isolates for the management of sugarcane smut. A total of 120 soil samples were collected from Kesem, Metehara, and Wonji sugarcane plantation fields. Consequently, 21 Trichoderma isolates were recovered from the samples, and in-vitro antagonist study of Trichoderma isolates against U. scitaminea was done using dual culture technique on fresh PDA. Among the native isolates, 12 of them revealed a pathogen suppression potential of >90% at 7 days after inoculation and most of them had higher pathogen suppression than the commercial isolate. However, five of the isolates were selected as potential candidates based on distinct suppression features, growth rate, and antagonistic performance. Identification of the five effective Trichoderma isolates (WI4-27, MKI2-k17, MI3-m33, WI12-25, and MKI3) was done based on macroscopic and microscopic characteristics. Thus, the isolates were identified as T. harzianum, T. asperellum, T. viride, T. viride, and T. harzianum, respectively. Accordingly, these isolates could be used as one component of integrated smut management practices once a study on compatibility with fungicides, in-vivo efficacy test against smut, and substrate selection for mass production of the isolates were commenced.http://dx.doi.org/10.1080/23311932.2021.1872853antagonistbiological controlmorphological featuressuppressiontrichoderma isolate |
| spellingShingle | Samuel Tegene Mashilla Dejene Habtamu Terefe Girma Tegegn Esayas Tena Amare Ayalew Evaluation of native Trichoderma isolates for the management of sugarcane smut (Ustilago scitaminea) in sugar plantations of Ethiopia Cogent Food & Agriculture antagonist biological control morphological features suppression trichoderma isolate |
| title | Evaluation of native Trichoderma isolates for the management of sugarcane smut (Ustilago scitaminea) in sugar plantations of Ethiopia |
| title_full | Evaluation of native Trichoderma isolates for the management of sugarcane smut (Ustilago scitaminea) in sugar plantations of Ethiopia |
| title_fullStr | Evaluation of native Trichoderma isolates for the management of sugarcane smut (Ustilago scitaminea) in sugar plantations of Ethiopia |
| title_full_unstemmed | Evaluation of native Trichoderma isolates for the management of sugarcane smut (Ustilago scitaminea) in sugar plantations of Ethiopia |
| title_short | Evaluation of native Trichoderma isolates for the management of sugarcane smut (Ustilago scitaminea) in sugar plantations of Ethiopia |
| title_sort | evaluation of native trichoderma isolates for the management of sugarcane smut ustilago scitaminea in sugar plantations of ethiopia |
| topic | antagonist biological control morphological features suppression trichoderma isolate |
| url | http://dx.doi.org/10.1080/23311932.2021.1872853 |
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