Evaluation of immunoserological detection of anti-liver kidney microsomal, anti-soluble liver antigen and anti-mitochondrial antibodies
Abstract Autoantibodies are the diagnostic hallmark of autoimmune liver diseases. Indirect immunofluorescence (IFT) is the reference method for the detection of anti-mitochondrial antibodies (AMA) and anti-liver kidney microsomal type-1 (anti-LKM1) antibodies, and inhibition ELISA (iELISA) for anti-...
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Nature Portfolio
2023-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-37095-z |
| _version_ | 1827917056288227328 |
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| author | Alejandro Campos-Murguia Nicole Henjes Stephanie Loges Heiner Wedemeyer Elmar Jaeckel Richard Taubert Bastian Engel |
| author_facet | Alejandro Campos-Murguia Nicole Henjes Stephanie Loges Heiner Wedemeyer Elmar Jaeckel Richard Taubert Bastian Engel |
| author_sort | Alejandro Campos-Murguia |
| collection | DOAJ |
| description | Abstract Autoantibodies are the diagnostic hallmark of autoimmune liver diseases. Indirect immunofluorescence (IFT) is the reference method for the detection of anti-mitochondrial antibodies (AMA) and anti-liver kidney microsomal type-1 (anti-LKM1) antibodies, and inhibition ELISA (iELISA) for anti-soluble liver antigen (anti-SLA) antibodies. Given the complexity of these techniques, commercial ELISAs have emerged as a practical alternative, but without head-to-head validations. This study evaluated the agreement between three commercial ELISAs and the reference techniques and the impact of polyreactive immunoglobulin G (pIgG), a recently described phenomenon in autoimmune hepatitis, on commercial ELISAs. Inter-rater reliability was assessed using Cohen-Kappa coefficient (κ). Forty-eight, 46, and 66 samples were analyzed for AMA, anti-LKM1, and anti-SLA, respectively. For AMA, one commercial assay showed high agreement (κ = 0.91 (0.78–1.00)) with the reference method, while the other two showed weak or moderate agreement. For anti-LKM1, only one commercial assay showed high agreement (κ = 0.86 (0.71–1.0)). For anti-SLA antibodies only moderate agreement was achieved (κ up to 0.71 (0.52–0.89)). There was a trend towards higher pIgG levels in false-positives in the commercial ELISAs. Patients with high suspicion of autoimmune liver diseases should be referred to reference laboratories with the capacity of performing gold standard methods if the initial ELISA-based screening was performed. |
| first_indexed | 2024-03-13T03:23:18Z |
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| id | doaj.art-8d9ac42811f34a7b95b746a234a1db07 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T03:23:18Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-8d9ac42811f34a7b95b746a234a1db072023-06-25T11:13:23ZengNature PortfolioScientific Reports2045-23222023-06-0113111110.1038/s41598-023-37095-zEvaluation of immunoserological detection of anti-liver kidney microsomal, anti-soluble liver antigen and anti-mitochondrial antibodiesAlejandro Campos-Murguia0Nicole Henjes1Stephanie Loges2Heiner Wedemeyer3Elmar Jaeckel4Richard Taubert5Bastian Engel6Department of Gastroenterology, Hepatology, Infectious Diseases and Endocrinology, Hannover Medical SchoolDepartment of Gastroenterology, Hepatology, Infectious Diseases and Endocrinology, Hannover Medical SchoolDepartment of Gastroenterology, Hepatology, Infectious Diseases and Endocrinology, Hannover Medical SchoolDepartment of Gastroenterology, Hepatology, Infectious Diseases and Endocrinology, Hannover Medical SchoolDepartment of Gastroenterology, Hepatology, Infectious Diseases and Endocrinology, Hannover Medical SchoolDepartment of Gastroenterology, Hepatology, Infectious Diseases and Endocrinology, Hannover Medical SchoolDepartment of Gastroenterology, Hepatology, Infectious Diseases and Endocrinology, Hannover Medical SchoolAbstract Autoantibodies are the diagnostic hallmark of autoimmune liver diseases. Indirect immunofluorescence (IFT) is the reference method for the detection of anti-mitochondrial antibodies (AMA) and anti-liver kidney microsomal type-1 (anti-LKM1) antibodies, and inhibition ELISA (iELISA) for anti-soluble liver antigen (anti-SLA) antibodies. Given the complexity of these techniques, commercial ELISAs have emerged as a practical alternative, but without head-to-head validations. This study evaluated the agreement between three commercial ELISAs and the reference techniques and the impact of polyreactive immunoglobulin G (pIgG), a recently described phenomenon in autoimmune hepatitis, on commercial ELISAs. Inter-rater reliability was assessed using Cohen-Kappa coefficient (κ). Forty-eight, 46, and 66 samples were analyzed for AMA, anti-LKM1, and anti-SLA, respectively. For AMA, one commercial assay showed high agreement (κ = 0.91 (0.78–1.00)) with the reference method, while the other two showed weak or moderate agreement. For anti-LKM1, only one commercial assay showed high agreement (κ = 0.86 (0.71–1.0)). For anti-SLA antibodies only moderate agreement was achieved (κ up to 0.71 (0.52–0.89)). There was a trend towards higher pIgG levels in false-positives in the commercial ELISAs. Patients with high suspicion of autoimmune liver diseases should be referred to reference laboratories with the capacity of performing gold standard methods if the initial ELISA-based screening was performed.https://doi.org/10.1038/s41598-023-37095-z |
| spellingShingle | Alejandro Campos-Murguia Nicole Henjes Stephanie Loges Heiner Wedemeyer Elmar Jaeckel Richard Taubert Bastian Engel Evaluation of immunoserological detection of anti-liver kidney microsomal, anti-soluble liver antigen and anti-mitochondrial antibodies Scientific Reports |
| title | Evaluation of immunoserological detection of anti-liver kidney microsomal, anti-soluble liver antigen and anti-mitochondrial antibodies |
| title_full | Evaluation of immunoserological detection of anti-liver kidney microsomal, anti-soluble liver antigen and anti-mitochondrial antibodies |
| title_fullStr | Evaluation of immunoserological detection of anti-liver kidney microsomal, anti-soluble liver antigen and anti-mitochondrial antibodies |
| title_full_unstemmed | Evaluation of immunoserological detection of anti-liver kidney microsomal, anti-soluble liver antigen and anti-mitochondrial antibodies |
| title_short | Evaluation of immunoserological detection of anti-liver kidney microsomal, anti-soluble liver antigen and anti-mitochondrial antibodies |
| title_sort | evaluation of immunoserological detection of anti liver kidney microsomal anti soluble liver antigen and anti mitochondrial antibodies |
| url | https://doi.org/10.1038/s41598-023-37095-z |
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