Comparison of the ability of exosomes and ectosomes derived from adipose-derived stromal cells to promote cartilage regeneration in a rat osteochondral defect model
Abstract Background Extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) offer promising prospects for stimulating cartilage regeneration. The different formation mechanisms suggest that exosomes and ectosomes possess different biological functions. However, little attention ha...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13287-024-03632-4 |
| _version_ | 1827377462412050432 |
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| author | Tengjing Xu Xinning Yu Kaiwang Xu Yunting Lin Jiajie Wang Zongyou Pan Jinghua Fang Siheng Wang Zhuxing Zhou Hongyun Song Sunan Zhu Xuesong Dai |
| author_facet | Tengjing Xu Xinning Yu Kaiwang Xu Yunting Lin Jiajie Wang Zongyou Pan Jinghua Fang Siheng Wang Zhuxing Zhou Hongyun Song Sunan Zhu Xuesong Dai |
| author_sort | Tengjing Xu |
| collection | DOAJ |
| description | Abstract Background Extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) offer promising prospects for stimulating cartilage regeneration. The different formation mechanisms suggest that exosomes and ectosomes possess different biological functions. However, little attention has been paid to the differential effects of EV subsets on cartilage regeneration. Methods Our study compared the effects of the two EVs isolated from adipose-derived MSCs (ASCs) on chondrocytes and bone marrow–derived MSCs (BMSCs) in vitro. Additionally, we loaded the two EVs into type I collagen hydrogels to optimize their application for the treatment of osteochondral defects in vivo. Results In vitro experiments demonstrate that ASC-derived exosomes (ASC-Exos) significantly promoted the proliferation and migration of both cells more effectively than ASC-derived ectosomes (ASC-Ectos). Furthermore, ASC-Exos facilitated a stronger differentiation of BMSCs into chondrogenic cells than ASC-Ectos, but both inhibited chondrocyte apoptosis to a similar extent. In the osteochondral defect model of rats, ASC-Exos promoted cartilage regeneration in situ better than ASC-Ectos. At 8 weeks, the hydrogel containing exosomes group (Gel + Exo group) had higher macroscopic and histological scores, a higher value of trabecular bone volume fraction (BV/TV), a lower value of trabecular thickness (Tb.Sp), and a better remodeling of extracellular matrix than the hydrogel containing ectosomes group (Gel + Ecto group). At 4 and 8 weeks, the expression of CD206 and Arginase-1 in the Gel + Exo group was significantly higher than that in the Gel + Ecto group. Conclusion Our findings indicate that administering ASC-Exos may be a more effective EV strategy for cartilage regeneration than the administration of ASC-Ectos. |
| first_indexed | 2024-03-08T12:39:33Z |
| format | Article |
| id | doaj.art-8d9c79f37d8a44ec98405d0c618f3b74 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-03-08T12:39:33Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-8d9c79f37d8a44ec98405d0c618f3b742024-01-21T12:12:56ZengBMCStem Cell Research & Therapy1757-65122024-01-0115112010.1186/s13287-024-03632-4Comparison of the ability of exosomes and ectosomes derived from adipose-derived stromal cells to promote cartilage regeneration in a rat osteochondral defect modelTengjing Xu0Xinning Yu1Kaiwang Xu2Yunting Lin3Jiajie Wang4Zongyou Pan5Jinghua Fang6Siheng Wang7Zhuxing Zhou8Hongyun Song9Sunan Zhu10Xuesong Dai11Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of MedicineDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of MedicineDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of MedicineDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of MedicineDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of MedicineDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of MedicineDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of MedicineDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of MedicineDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of MedicineDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of MedicineDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of MedicineDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of MedicineAbstract Background Extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) offer promising prospects for stimulating cartilage regeneration. The different formation mechanisms suggest that exosomes and ectosomes possess different biological functions. However, little attention has been paid to the differential effects of EV subsets on cartilage regeneration. Methods Our study compared the effects of the two EVs isolated from adipose-derived MSCs (ASCs) on chondrocytes and bone marrow–derived MSCs (BMSCs) in vitro. Additionally, we loaded the two EVs into type I collagen hydrogels to optimize their application for the treatment of osteochondral defects in vivo. Results In vitro experiments demonstrate that ASC-derived exosomes (ASC-Exos) significantly promoted the proliferation and migration of both cells more effectively than ASC-derived ectosomes (ASC-Ectos). Furthermore, ASC-Exos facilitated a stronger differentiation of BMSCs into chondrogenic cells than ASC-Ectos, but both inhibited chondrocyte apoptosis to a similar extent. In the osteochondral defect model of rats, ASC-Exos promoted cartilage regeneration in situ better than ASC-Ectos. At 8 weeks, the hydrogel containing exosomes group (Gel + Exo group) had higher macroscopic and histological scores, a higher value of trabecular bone volume fraction (BV/TV), a lower value of trabecular thickness (Tb.Sp), and a better remodeling of extracellular matrix than the hydrogel containing ectosomes group (Gel + Ecto group). At 4 and 8 weeks, the expression of CD206 and Arginase-1 in the Gel + Exo group was significantly higher than that in the Gel + Ecto group. Conclusion Our findings indicate that administering ASC-Exos may be a more effective EV strategy for cartilage regeneration than the administration of ASC-Ectos.https://doi.org/10.1186/s13287-024-03632-4Cartilage regenerationMesenchymal stromal cellsExosomesEctosomesMacrophages |
| spellingShingle | Tengjing Xu Xinning Yu Kaiwang Xu Yunting Lin Jiajie Wang Zongyou Pan Jinghua Fang Siheng Wang Zhuxing Zhou Hongyun Song Sunan Zhu Xuesong Dai Comparison of the ability of exosomes and ectosomes derived from adipose-derived stromal cells to promote cartilage regeneration in a rat osteochondral defect model Stem Cell Research & Therapy Cartilage regeneration Mesenchymal stromal cells Exosomes Ectosomes Macrophages |
| title | Comparison of the ability of exosomes and ectosomes derived from adipose-derived stromal cells to promote cartilage regeneration in a rat osteochondral defect model |
| title_full | Comparison of the ability of exosomes and ectosomes derived from adipose-derived stromal cells to promote cartilage regeneration in a rat osteochondral defect model |
| title_fullStr | Comparison of the ability of exosomes and ectosomes derived from adipose-derived stromal cells to promote cartilage regeneration in a rat osteochondral defect model |
| title_full_unstemmed | Comparison of the ability of exosomes and ectosomes derived from adipose-derived stromal cells to promote cartilage regeneration in a rat osteochondral defect model |
| title_short | Comparison of the ability of exosomes and ectosomes derived from adipose-derived stromal cells to promote cartilage regeneration in a rat osteochondral defect model |
| title_sort | comparison of the ability of exosomes and ectosomes derived from adipose derived stromal cells to promote cartilage regeneration in a rat osteochondral defect model |
| topic | Cartilage regeneration Mesenchymal stromal cells Exosomes Ectosomes Macrophages |
| url | https://doi.org/10.1186/s13287-024-03632-4 |
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