Mutation and Lineage Analysis of DNMT3A in BCR-ABL1-negative Chronic Myeloproliferative Neoplasms
In addition to the JAK2 V617F mutation, somatic mutation in DNMT3A has been described in BCL-ABL1-negative myeloproliferative neoplasms (MPNs). We have screened for DNMT3A exon 23 mutations in 130 adult Taiwanese patients with chronic phase myeloproliferative neoplasms. Only one somatic DNMT3A R882H...
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| Language: | English |
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Taiwan Society of Geriatric Emergency and Critical Medicine (TSGECM)
2013-09-01
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| Series: | International Journal of Gerontology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873959813000069 |
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| author | Huan-Chau Lin Shu-Ching Wang Caleb Gon-Shen Chen Ming-Chih Chang Wei-Ting Wang Nai-Wen Su Hung-I Cheng Johnson Lin Yi-Fang Chang Ruey-Kuen Hsieh Chien-Chung Chang Yuchi Hwang Ken-Hong Lim Yuan-Yeh Kuo |
| author_facet | Huan-Chau Lin Shu-Ching Wang Caleb Gon-Shen Chen Ming-Chih Chang Wei-Ting Wang Nai-Wen Su Hung-I Cheng Johnson Lin Yi-Fang Chang Ruey-Kuen Hsieh Chien-Chung Chang Yuchi Hwang Ken-Hong Lim Yuan-Yeh Kuo |
| author_sort | Huan-Chau Lin |
| collection | DOAJ |
| description | In addition to the JAK2 V617F mutation, somatic mutation in DNMT3A has been described in BCL-ABL1-negative myeloproliferative neoplasms (MPNs). We have screened for DNMT3A exon 23 mutations in 130 adult Taiwanese patients with chronic phase myeloproliferative neoplasms. Only one somatic DNMT3A R882H mutation was identified in one JAK2 V617F mutation-positive essential thrombocythemia patient (1/91, 1%). Both mutations were detected in the CD34+-, CD19+-, peripheral blood mononuclear cell- and granulocyte-enriched fractions, but were not detected in the CD3+-enriched fraction by lineage analysis. Our findings suggest that DNMT3A mutation is not prevalent in MPNs, and further study is needed to clarify its role in the molecular pathogenesis of myeloproliferative neoplasms. |
| first_indexed | 2024-12-19T07:48:53Z |
| format | Article |
| id | doaj.art-8da35d0012b24a9892ca26cdea777184 |
| institution | Directory Open Access Journal |
| issn | 1873-9598 |
| language | English |
| last_indexed | 2024-12-19T07:48:53Z |
| publishDate | 2013-09-01 |
| publisher | Taiwan Society of Geriatric Emergency and Critical Medicine (TSGECM) |
| record_format | Article |
| series | International Journal of Gerontology |
| spelling | doaj.art-8da35d0012b24a9892ca26cdea7771842022-12-21T20:30:14ZengTaiwan Society of Geriatric Emergency and Critical Medicine (TSGECM)International Journal of Gerontology1873-95982013-09-017318618810.1016/j.ijge.2013.01.005Mutation and Lineage Analysis of DNMT3A in BCR-ABL1-negative Chronic Myeloproliferative NeoplasmsHuan-Chau Lin0Shu-Ching Wang1Caleb Gon-Shen Chen2Ming-Chih Chang3Wei-Ting Wang4Nai-Wen Su5Hung-I Cheng6Johnson Lin7Yi-Fang Chang8Ruey-Kuen Hsieh9Chien-Chung Chang10Yuchi Hwang11Ken-Hong Lim12Yuan-Yeh Kuo13Division of Hematology and Oncology, Department of Internal Medicine, Mackay Memorial Hospital, Taipei, TaiwanInstitute of Molecular and Cellular Biology, National Tsing Hua University, TaiwanDivision of Hematology and Oncology, Department of Internal Medicine, Mackay Memorial Hospital, Taipei, TaiwanDivision of Hematology and Oncology, Department of Internal Medicine, Mackay Memorial Hospital, Taipei, TaiwanLaboratory of Good Clinical Research Center, Department of Medical Research, Mackay Memorial Hospital, Tamsui District, New Taipei City, TaiwanDivision of Hematology and Oncology, Department of Internal Medicine, Mackay Memorial Hospital, Taipei, TaiwanDivision of Hematology, Department of Internal Medicine, Mackay Memorial Hospital, Hsinchu, TaiwanDivision of Hematology and Oncology, Department of Internal Medicine, Mackay Memorial Hospital, Taipei, TaiwanDivision of Hematology and Oncology, Department of Internal Medicine, Mackay Memorial Hospital, Taipei, TaiwanDivision of Hematology and Oncology, Department of Internal Medicine, Mackay Memorial Hospital, Taipei, TaiwanInstitute of Molecular and Cellular Biology, National Tsing Hua University, TaiwanVita Genomics, Inc., New Taipei City, TaiwanDivision of Hematology and Oncology, Department of Internal Medicine, Mackay Memorial Hospital, Taipei, TaiwanGraduate Institute of Oncology, National Taiwan University, College of Medicine, Taipei, TaiwanIn addition to the JAK2 V617F mutation, somatic mutation in DNMT3A has been described in BCL-ABL1-negative myeloproliferative neoplasms (MPNs). We have screened for DNMT3A exon 23 mutations in 130 adult Taiwanese patients with chronic phase myeloproliferative neoplasms. Only one somatic DNMT3A R882H mutation was identified in one JAK2 V617F mutation-positive essential thrombocythemia patient (1/91, 1%). Both mutations were detected in the CD34+-, CD19+-, peripheral blood mononuclear cell- and granulocyte-enriched fractions, but were not detected in the CD3+-enriched fraction by lineage analysis. Our findings suggest that DNMT3A mutation is not prevalent in MPNs, and further study is needed to clarify its role in the molecular pathogenesis of myeloproliferative neoplasms.http://www.sciencedirect.com/science/article/pii/S1873959813000069DNMT3Amutationmyeloproliferative neoplasm |
| spellingShingle | Huan-Chau Lin Shu-Ching Wang Caleb Gon-Shen Chen Ming-Chih Chang Wei-Ting Wang Nai-Wen Su Hung-I Cheng Johnson Lin Yi-Fang Chang Ruey-Kuen Hsieh Chien-Chung Chang Yuchi Hwang Ken-Hong Lim Yuan-Yeh Kuo Mutation and Lineage Analysis of DNMT3A in BCR-ABL1-negative Chronic Myeloproliferative Neoplasms International Journal of Gerontology DNMT3A mutation myeloproliferative neoplasm |
| title | Mutation and Lineage Analysis of DNMT3A in BCR-ABL1-negative Chronic Myeloproliferative Neoplasms |
| title_full | Mutation and Lineage Analysis of DNMT3A in BCR-ABL1-negative Chronic Myeloproliferative Neoplasms |
| title_fullStr | Mutation and Lineage Analysis of DNMT3A in BCR-ABL1-negative Chronic Myeloproliferative Neoplasms |
| title_full_unstemmed | Mutation and Lineage Analysis of DNMT3A in BCR-ABL1-negative Chronic Myeloproliferative Neoplasms |
| title_short | Mutation and Lineage Analysis of DNMT3A in BCR-ABL1-negative Chronic Myeloproliferative Neoplasms |
| title_sort | mutation and lineage analysis of dnmt3a in bcr abl1 negative chronic myeloproliferative neoplasms |
| topic | DNMT3A mutation myeloproliferative neoplasm |
| url | http://www.sciencedirect.com/science/article/pii/S1873959813000069 |
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