HPLC-DAD and UHPLC/QTOF-MS Analysis of Polyphenols in Extracts of the African Species <i>Combretum</i> <i>padoides</i>, <i>C. zeyheri</i> and <i>C. psidioides</i> Related to Their Antimycobacterial Activity
<i>Combretum padoides</i> Engl. & Diels, <i>C. psidioides</i> Welv. and <i>C. zeyheri</i> Sond. are used for the treatment of infections and tuberculosis related symptoms in African traditional medicine. In order to verify these uses, extracts were screened fo...
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MDPI AG
2020-07-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/8/459 |
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| author | Pia Fyhrquist Enass Y. A. Salih Satu Helenius Into Laakso Riitta Julkunen-Tiitto |
| author_facet | Pia Fyhrquist Enass Y. A. Salih Satu Helenius Into Laakso Riitta Julkunen-Tiitto |
| author_sort | Pia Fyhrquist |
| collection | DOAJ |
| description | <i>Combretum padoides</i> Engl. & Diels, <i>C. psidioides</i> Welv. and <i>C. zeyheri</i> Sond. are used for the treatment of infections and tuberculosis related symptoms in African traditional medicine. In order to verify these uses, extracts were screened for their growth inhibitory effects against <i>M. smegmatis</i> ATCC 14468. Ultra-high pressure liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UHPLC/QTOF-MS) and GC-MS were used to investigate the polyphenolic composition in the active extracts. The lowest minimum inhibitory concentration (MIC), 625 µg/mL, was shown by a methanol extract of the stem bark of <i>C. psidioides</i>. A butanol extract of <i>C. psidioides</i> gave large inhibition zone diameters (IZD 21 mm) and inhibited 84% of the mycobacterial growth at 312 µg/mL. Combretastatin B-2 and dihydrostilbene derivatives were present in the methanol extract of <i>C. psidioides</i>, whereas the butanol extract of this species contained punicalagin, corilagin, and sanguiin H-4. Methanol and butanol extracts of the stem bark of <i>C. padoides</i> gave large inhibition zone diameters (IZD 26.5 mm) and MIC values of 1250 and 2500 µg/mL, respectively. <i>C. padoides</i> contained an ellagitannin with a mass identical to punicalagin ([M-H]<sup>−</sup> 1083.0587) and a corilagin like derivative ([M-H]<sup>−</sup> 633.0750) as well as ellagic acid arabinoside and methyl ellagic acid xyloside. A butanol extract of the roots of <i>C. zeyheri</i> showed mild antimycobacterial activity and contained a gallotannin at <i>m</i>/<i>z</i> [M-H]<sup>−</sup> 647.0894 as the main compound along with punicalagin and three unknown ellagitannins at <i>m</i>/<i>z</i> [M-H]<sup>−</sup> 763.0788, 765.0566, and 817.4212. Our results indicate that the studied species of <i>Combretum</i> contain phenolic and polyphenolic compounds with possible potential as leads for antimycobacterial drugs or as adjuvants for conventional anti-TB drugs. |
| first_indexed | 2024-03-10T18:07:30Z |
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| spelling | doaj.art-8da44c9f8e334cfea2632fcc51572ba22023-11-20T08:22:51ZengMDPI AGAntibiotics2079-63822020-07-019845910.3390/antibiotics9080459HPLC-DAD and UHPLC/QTOF-MS Analysis of Polyphenols in Extracts of the African Species <i>Combretum</i> <i>padoides</i>, <i>C. zeyheri</i> and <i>C. psidioides</i> Related to Their Antimycobacterial ActivityPia Fyhrquist0Enass Y. A. Salih1Satu Helenius2Into Laakso3Riitta Julkunen-Tiitto4Faculty of Pharmacy, Division of Pharmaceutical Biosciences, Viikki Biocenter, P.O. Box 56, FIN-00014, University of Helsinki, 00100 Helsinki, FinlandFaculty of Pharmacy, Division of Pharmaceutical Biosciences, Viikki Biocenter, P.O. Box 56, FIN-00014, University of Helsinki, 00100 Helsinki, FinlandFaculty of Pharmacy, Division of Pharmaceutical Biosciences, Viikki Biocenter, P.O. Box 56, FIN-00014, University of Helsinki, 00100 Helsinki, FinlandFaculty of Pharmacy, Division of Pharmaceutical Biosciences, Viikki Biocenter, P.O. Box 56, FIN-00014, University of Helsinki, 00100 Helsinki, FinlandFaculty of Science and Forestry, Department of Environmental and Biological Sciences, University of Eastern Finland, 80101 Joensuu, Finland<i>Combretum padoides</i> Engl. & Diels, <i>C. psidioides</i> Welv. and <i>C. zeyheri</i> Sond. are used for the treatment of infections and tuberculosis related symptoms in African traditional medicine. In order to verify these uses, extracts were screened for their growth inhibitory effects against <i>M. smegmatis</i> ATCC 14468. Ultra-high pressure liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UHPLC/QTOF-MS) and GC-MS were used to investigate the polyphenolic composition in the active extracts. The lowest minimum inhibitory concentration (MIC), 625 µg/mL, was shown by a methanol extract of the stem bark of <i>C. psidioides</i>. A butanol extract of <i>C. psidioides</i> gave large inhibition zone diameters (IZD 21 mm) and inhibited 84% of the mycobacterial growth at 312 µg/mL. Combretastatin B-2 and dihydrostilbene derivatives were present in the methanol extract of <i>C. psidioides</i>, whereas the butanol extract of this species contained punicalagin, corilagin, and sanguiin H-4. Methanol and butanol extracts of the stem bark of <i>C. padoides</i> gave large inhibition zone diameters (IZD 26.5 mm) and MIC values of 1250 and 2500 µg/mL, respectively. <i>C. padoides</i> contained an ellagitannin with a mass identical to punicalagin ([M-H]<sup>−</sup> 1083.0587) and a corilagin like derivative ([M-H]<sup>−</sup> 633.0750) as well as ellagic acid arabinoside and methyl ellagic acid xyloside. A butanol extract of the roots of <i>C. zeyheri</i> showed mild antimycobacterial activity and contained a gallotannin at <i>m</i>/<i>z</i> [M-H]<sup>−</sup> 647.0894 as the main compound along with punicalagin and three unknown ellagitannins at <i>m</i>/<i>z</i> [M-H]<sup>−</sup> 763.0788, 765.0566, and 817.4212. Our results indicate that the studied species of <i>Combretum</i> contain phenolic and polyphenolic compounds with possible potential as leads for antimycobacterial drugs or as adjuvants for conventional anti-TB drugs.https://www.mdpi.com/2079-6382/9/8/459<i>Combretum</i> spp.antimycobacterialhydrolysable tanninsstilbenesAfrican traditional medicine |
| spellingShingle | Pia Fyhrquist Enass Y. A. Salih Satu Helenius Into Laakso Riitta Julkunen-Tiitto HPLC-DAD and UHPLC/QTOF-MS Analysis of Polyphenols in Extracts of the African Species <i>Combretum</i> <i>padoides</i>, <i>C. zeyheri</i> and <i>C. psidioides</i> Related to Their Antimycobacterial Activity Antibiotics <i>Combretum</i> spp. antimycobacterial hydrolysable tannins stilbenes African traditional medicine |
| title | HPLC-DAD and UHPLC/QTOF-MS Analysis of Polyphenols in Extracts of the African Species <i>Combretum</i> <i>padoides</i>, <i>C. zeyheri</i> and <i>C. psidioides</i> Related to Their Antimycobacterial Activity |
| title_full | HPLC-DAD and UHPLC/QTOF-MS Analysis of Polyphenols in Extracts of the African Species <i>Combretum</i> <i>padoides</i>, <i>C. zeyheri</i> and <i>C. psidioides</i> Related to Their Antimycobacterial Activity |
| title_fullStr | HPLC-DAD and UHPLC/QTOF-MS Analysis of Polyphenols in Extracts of the African Species <i>Combretum</i> <i>padoides</i>, <i>C. zeyheri</i> and <i>C. psidioides</i> Related to Their Antimycobacterial Activity |
| title_full_unstemmed | HPLC-DAD and UHPLC/QTOF-MS Analysis of Polyphenols in Extracts of the African Species <i>Combretum</i> <i>padoides</i>, <i>C. zeyheri</i> and <i>C. psidioides</i> Related to Their Antimycobacterial Activity |
| title_short | HPLC-DAD and UHPLC/QTOF-MS Analysis of Polyphenols in Extracts of the African Species <i>Combretum</i> <i>padoides</i>, <i>C. zeyheri</i> and <i>C. psidioides</i> Related to Their Antimycobacterial Activity |
| title_sort | hplc dad and uhplc qtof ms analysis of polyphenols in extracts of the african species i combretum i i padoides i i c zeyheri i and i c psidioides i related to their antimycobacterial activity |
| topic | <i>Combretum</i> spp. antimycobacterial hydrolysable tannins stilbenes African traditional medicine |
| url | https://www.mdpi.com/2079-6382/9/8/459 |
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