Evaluate Risk Analysis of Measurement Uncertainty (MU) of Different Methods Applied for Veterinary Vaccines Evaluation
Veterinary Serum and Vaccine Research Institute (VSVRI ) objected to producing highly efficient vaccines from reference or local isolates according to the international specifications for protecting animals and poultry against different diseases and preparing combined vaccines to save effort, time a...
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| Format: | Article |
| Language: | English |
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Egyptian Society for Animal Management
2022-10-01
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| Series: | Journal of Applied Veterinary Sciences |
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| Online Access: | https://javs.journals.ekb.eg/article_257155_da1a985a7a398ccd29f308579d19421d.pdf |
| _version_ | 1811258184450441216 |
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| author | Hala Elsawy Amany El Zieny H. M. El Naggar Doaa Rady Eman Zaki Fouad El tahaan |
| author_facet | Hala Elsawy Amany El Zieny H. M. El Naggar Doaa Rady Eman Zaki Fouad El tahaan |
| author_sort | Hala Elsawy |
| collection | DOAJ |
| description | Veterinary Serum and Vaccine Research Institute (VSVRI ) objected to producing highly efficient vaccines from reference or local isolates according to the international specifications for protecting animals and poultry against different diseases and preparing combined vaccines to save effort, time and money. VSVRI produces various types of vaccines such as live attenuated viral poultry, live attenuated viral animal vaccines, live attenuated Bacterial animal vaccines and Inactivated viral animal vaccines. These vaccines are subjected to quality assurance through seven methods with different techniques. Validity and measurement uncertainty for results were applied in the evaluation of results of the following: Titration of virus content using egg inoculation through Chorioallantoic Membrane (CAM), Intra-allantoic (IA) and Tissue Culture (TC). Determination of antibody titer using ELISA, HI and VNT techniques. Finally, enumeration of aerobic bacterial count for living attenuated Bacterial vaccine by culture technique. Homogeneity and stability results for all vaccines were accepted criteria according to TS/ ISO 22117. The reproducibility component of the TC technique was higher while the Bias was lower than other different routes of egg inoculation. In conclusion, the accuracy of TC technique is better than the egg inoculation technique which will reflect on the measurement of uncertainty. There is no significant change in the final measurement uncertainty of different routes of egg inoculation. In comparison, there is a variance between bias accuracy and reproducibility precision due to the equation of measurement of uncertainty depending on all processes performed in test accuracy and precession. |
| first_indexed | 2024-04-12T18:09:17Z |
| format | Article |
| id | doaj.art-8da6b6646aa241e28d1693d522fc0bf8 |
| institution | Directory Open Access Journal |
| issn | 1687-4072 2090-3308 |
| language | English |
| last_indexed | 2024-04-12T18:09:17Z |
| publishDate | 2022-10-01 |
| publisher | Egyptian Society for Animal Management |
| record_format | Article |
| series | Journal of Applied Veterinary Sciences |
| spelling | doaj.art-8da6b6646aa241e28d1693d522fc0bf82022-12-22T03:21:53ZengEgyptian Society for Animal ManagementJournal of Applied Veterinary Sciences1687-40722090-33082022-10-0174445410.21608/javs.2022.150328.1163257155Evaluate Risk Analysis of Measurement Uncertainty (MU) of Different Methods Applied for Veterinary Vaccines EvaluationHala Elsawy0Amany El Zieny1H. M. El Naggar2Doaa Rady3Eman Zaki4Fouad El tahaan5Department of Quality Control Lab (QCL) Veterinary Serum and Vaccine Research Institute (VSVRI), Abbasia, Cairo, Agricultural Research Center (ARC), EgyptDepartment of Quality Control Lab (QCL) Veterinary Serum and Vaccine Research Institute (VSVRI), Abbasia, Cairo, Agricultural Research Center (ARC), EgyptDepartment of Quality Control Lab (QCL) Veterinary Serum and Vaccine Research Institute (VSVRI), Abbasia, Cairo, Agricultural Research Center (ARC), EgyptDepartment of Quality Control Lab (QCL) Veterinary Serum and Vaccine Research Institute (VSVRI), Abbasia, Cairo, Agricultural Research Center (ARC), EgyptDepartment of Quality Control Lab (QCL) Veterinary Serum and Vaccine Research Institute (VSVRI), Abbasia, Cairo, Agricultural Research Center (ARC), EgyptCentral Laboratory for Evaluation of Veterinary Biologics (CLEVB), Abbasia, Cairo, Agricultural Research Center (ARC), EgyptVeterinary Serum and Vaccine Research Institute (VSVRI ) objected to producing highly efficient vaccines from reference or local isolates according to the international specifications for protecting animals and poultry against different diseases and preparing combined vaccines to save effort, time and money. VSVRI produces various types of vaccines such as live attenuated viral poultry, live attenuated viral animal vaccines, live attenuated Bacterial animal vaccines and Inactivated viral animal vaccines. These vaccines are subjected to quality assurance through seven methods with different techniques. Validity and measurement uncertainty for results were applied in the evaluation of results of the following: Titration of virus content using egg inoculation through Chorioallantoic Membrane (CAM), Intra-allantoic (IA) and Tissue Culture (TC). Determination of antibody titer using ELISA, HI and VNT techniques. Finally, enumeration of aerobic bacterial count for living attenuated Bacterial vaccine by culture technique. Homogeneity and stability results for all vaccines were accepted criteria according to TS/ ISO 22117. The reproducibility component of the TC technique was higher while the Bias was lower than other different routes of egg inoculation. In conclusion, the accuracy of TC technique is better than the egg inoculation technique which will reflect on the measurement of uncertainty. There is no significant change in the final measurement uncertainty of different routes of egg inoculation. In comparison, there is a variance between bias accuracy and reproducibility precision due to the equation of measurement of uncertainty depending on all processes performed in test accuracy and precession.https://javs.journals.ekb.eg/article_257155_da1a985a7a398ccd29f308579d19421d.pdfbias accuracy and reproducibility precisionmeasurement uncertaintyvaccine |
| spellingShingle | Hala Elsawy Amany El Zieny H. M. El Naggar Doaa Rady Eman Zaki Fouad El tahaan Evaluate Risk Analysis of Measurement Uncertainty (MU) of Different Methods Applied for Veterinary Vaccines Evaluation Journal of Applied Veterinary Sciences bias accuracy and reproducibility precision measurement uncertainty vaccine |
| title | Evaluate Risk Analysis of Measurement Uncertainty (MU) of Different Methods Applied for Veterinary Vaccines Evaluation |
| title_full | Evaluate Risk Analysis of Measurement Uncertainty (MU) of Different Methods Applied for Veterinary Vaccines Evaluation |
| title_fullStr | Evaluate Risk Analysis of Measurement Uncertainty (MU) of Different Methods Applied for Veterinary Vaccines Evaluation |
| title_full_unstemmed | Evaluate Risk Analysis of Measurement Uncertainty (MU) of Different Methods Applied for Veterinary Vaccines Evaluation |
| title_short | Evaluate Risk Analysis of Measurement Uncertainty (MU) of Different Methods Applied for Veterinary Vaccines Evaluation |
| title_sort | evaluate risk analysis of measurement uncertainty mu of different methods applied for veterinary vaccines evaluation |
| topic | bias accuracy and reproducibility precision measurement uncertainty vaccine |
| url | https://javs.journals.ekb.eg/article_257155_da1a985a7a398ccd29f308579d19421d.pdf |
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