Antifungal Activity and Biosynthetic Potential of New <i>Streptomyces</i> sp. MW-W600-10 Strain Isolated from Coal Mine Water
Crop infections by fungi lead to severe losses in food production and pose risks for human health. The increasing resistance of pathogens to fungicides has led to the higher usage of these chemicals, which burdens the environment and highlights the need to find novel natural biocontrol agents. Membe...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/14/7441 |
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| author | Piotr Siupka Frederik Teilfeldt Hansen Aleksandra Schier Simone Rocco Trine Sørensen Zofia Piotrowska-Seget |
| author_facet | Piotr Siupka Frederik Teilfeldt Hansen Aleksandra Schier Simone Rocco Trine Sørensen Zofia Piotrowska-Seget |
| author_sort | Piotr Siupka |
| collection | DOAJ |
| description | Crop infections by fungi lead to severe losses in food production and pose risks for human health. The increasing resistance of pathogens to fungicides has led to the higher usage of these chemicals, which burdens the environment and highlights the need to find novel natural biocontrol agents. Members of the genus <i>Streptomyces</i> are known to produce a plethora of bioactive compounds. Recently, researchers have turned to extreme and previously unexplored niches in the search for new strains with antimicrobial activities. One such niche are underground coal mine environments. We isolated the new <i>Streptomyces</i> sp. MW-W600-10 strain from coal mine water samples collected at 665 m below ground level. We examined the antifungal activity of the strain against plant pathogens <i>Fusarium culmorum</i> DSM62188 and <i>Nigrospora oryzae</i> roseF7. Furthermore, we analyzed the strain’s biosynthetic potential with the antiSMASH tool. The strain showed inhibitory activity against both fungi strains. Genome mining revealed that it has 39 BGCs, among which 13 did not show similarity to those in databases. Additionally, we examined the activity of the <i>Streptomyces</i> sp. S-2 strain isolated from black soot against <i>F. culmorum</i> DSM62188. These results show that coal-related strains could be a source of novel bioactive compounds. Future studies will elucidate their full biotechnological potential. |
| first_indexed | 2024-03-10T09:37:26Z |
| format | Article |
| id | doaj.art-8da957b82727470c932d9f2518c9827d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T09:37:26Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8da957b82727470c932d9f2518c9827d2023-11-22T03:58:38ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-012214744110.3390/ijms22147441Antifungal Activity and Biosynthetic Potential of New <i>Streptomyces</i> sp. MW-W600-10 Strain Isolated from Coal Mine WaterPiotr Siupka0Frederik Teilfeldt Hansen1Aleksandra Schier2Simone Rocco3Trine Sørensen4Zofia Piotrowska-Seget5Faculty of Natural Sciences, Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, 40032 Katowice, PolandFaculty of Engineering and Science, Department of Chemistry and Biosciences, University of Aalborg, 9220 Aalborg, DenmarkFaculty of Natural Sciences, Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, 40032 Katowice, PolandFaculty of Natural Sciences, Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, 40032 Katowice, PolandFaculty of Engineering and Science, Department of Chemistry and Biosciences, University of Aalborg, 9220 Aalborg, DenmarkFaculty of Natural Sciences, Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, 40032 Katowice, PolandCrop infections by fungi lead to severe losses in food production and pose risks for human health. The increasing resistance of pathogens to fungicides has led to the higher usage of these chemicals, which burdens the environment and highlights the need to find novel natural biocontrol agents. Members of the genus <i>Streptomyces</i> are known to produce a plethora of bioactive compounds. Recently, researchers have turned to extreme and previously unexplored niches in the search for new strains with antimicrobial activities. One such niche are underground coal mine environments. We isolated the new <i>Streptomyces</i> sp. MW-W600-10 strain from coal mine water samples collected at 665 m below ground level. We examined the antifungal activity of the strain against plant pathogens <i>Fusarium culmorum</i> DSM62188 and <i>Nigrospora oryzae</i> roseF7. Furthermore, we analyzed the strain’s biosynthetic potential with the antiSMASH tool. The strain showed inhibitory activity against both fungi strains. Genome mining revealed that it has 39 BGCs, among which 13 did not show similarity to those in databases. Additionally, we examined the activity of the <i>Streptomyces</i> sp. S-2 strain isolated from black soot against <i>F. culmorum</i> DSM62188. These results show that coal-related strains could be a source of novel bioactive compounds. Future studies will elucidate their full biotechnological potential.https://www.mdpi.com/1422-0067/22/14/7441antifungal activitybiosynthetic gene clustersgenome miningcoal-related environments<i>Streptomyces</i>quantitative PCR |
| spellingShingle | Piotr Siupka Frederik Teilfeldt Hansen Aleksandra Schier Simone Rocco Trine Sørensen Zofia Piotrowska-Seget Antifungal Activity and Biosynthetic Potential of New <i>Streptomyces</i> sp. MW-W600-10 Strain Isolated from Coal Mine Water International Journal of Molecular Sciences antifungal activity biosynthetic gene clusters genome mining coal-related environments <i>Streptomyces</i> quantitative PCR |
| title | Antifungal Activity and Biosynthetic Potential of New <i>Streptomyces</i> sp. MW-W600-10 Strain Isolated from Coal Mine Water |
| title_full | Antifungal Activity and Biosynthetic Potential of New <i>Streptomyces</i> sp. MW-W600-10 Strain Isolated from Coal Mine Water |
| title_fullStr | Antifungal Activity and Biosynthetic Potential of New <i>Streptomyces</i> sp. MW-W600-10 Strain Isolated from Coal Mine Water |
| title_full_unstemmed | Antifungal Activity and Biosynthetic Potential of New <i>Streptomyces</i> sp. MW-W600-10 Strain Isolated from Coal Mine Water |
| title_short | Antifungal Activity and Biosynthetic Potential of New <i>Streptomyces</i> sp. MW-W600-10 Strain Isolated from Coal Mine Water |
| title_sort | antifungal activity and biosynthetic potential of new i streptomyces i sp mw w600 10 strain isolated from coal mine water |
| topic | antifungal activity biosynthetic gene clusters genome mining coal-related environments <i>Streptomyces</i> quantitative PCR |
| url | https://www.mdpi.com/1422-0067/22/14/7441 |
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