Elevated circulating GPHB5 levels in women with insulin resistance and polycystic ovary syndrome: A cross-sectional study and multiple intervention studies
ObjectiveGPHB5 has been found to be associated with glucose and lipid metabolism in animal studies. However, the association of GPHB5 with IR and metabolic disorders remains unknown, and there is a lack of research in humans. Our aim in this study was to investigate the relationship between circulat...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Endocrinology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.1010714/full |
| _version_ | 1811185161765650432 |
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| author | Yanping Wang Yanping Wang Ting Xiang Xuyun Xia Hongmin Zhang Shan Geng Gangyi Yang Sheng Qiu Yirui He Rui Liu Ling Li Hua Liu Ke Li Lili Zhang Zerong Liang Jianguo He |
| author_facet | Yanping Wang Yanping Wang Ting Xiang Xuyun Xia Hongmin Zhang Shan Geng Gangyi Yang Sheng Qiu Yirui He Rui Liu Ling Li Hua Liu Ke Li Lili Zhang Zerong Liang Jianguo He |
| author_sort | Yanping Wang |
| collection | DOAJ |
| description | ObjectiveGPHB5 has been found to be associated with glucose and lipid metabolism in animal studies. However, the association of GPHB5 with IR and metabolic disorders remains unknown, and there is a lack of research in humans. Our aim in this study was to investigate the relationship between circulating GPHB5 and metabolic disorders in humans.MethodsBioinformatics analysis was performed to understand the relationship between GPHB5 and metabolic disorders. GPHB5 mRNA expression in mice and rats was determined using RT-qPCR. Circulating GPHB5 concentrations were measured with an ELISA kit. EHC and OGTT were performed in humans.ResultsBioinformatics analysis shows that GPHB5 is associated with metabolic disorders and PCOS. GPHB5 mRNA expression levels in the metabolic-related tissues of HFD-fed mice, db/db and ob/ob mice, and PCOS rats were significantly higher than those of WT mice or rats. In human studies, we find that circulating GPHB5 levels were significantly higher in women with IR and PCOS. GPHB5 levels were positively correlated with age, BMI, WHR, BP, FBG, 2 h-BG, FIns, 2 h-Ins, TC, LDL-C, HbA1c, and FFA, but negatively correlated with adiponectin. Furthermore, GPHB5 was positively correlated with DHEAS and FAI, while negatively correlated with SHBG, FSH, SHBG and FSH. The increased GPHB5 concentration was related to IR and PCOS. After the treatment of metformin, GLP-1RA (Lira), and TZDs, circulating GPHB5 levels were decreased.ConclusionsOur results reveal that circulating GPHB5 could be a biomarker and potential therapeutic target for IR and PCOS in women. |
| first_indexed | 2024-04-11T13:24:56Z |
| format | Article |
| id | doaj.art-8daf63b18eee472ba204c2c987a0143c |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-11T13:24:56Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-8daf63b18eee472ba204c2c987a0143c2022-12-22T04:22:06ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-12-011310.3389/fendo.2022.10107141010714Elevated circulating GPHB5 levels in women with insulin resistance and polycystic ovary syndrome: A cross-sectional study and multiple intervention studiesYanping Wang0Yanping Wang1Ting Xiang2Xuyun Xia3Hongmin Zhang4Shan Geng5Gangyi Yang6Sheng Qiu7Yirui He8Rui Liu9Ling Li10Hua Liu11Ke Li12Lili Zhang13Zerong Liang14Jianguo He15Department of Endocrinology, Chongqing Red Cross Hospital (People’s Hospital of Jiangbei District), Chongqing, ChinaDepartment of Endocrinology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, ChinaDepartment of Endocrinology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, ChinaDepartment of Endocrinology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, ChinaDepartment of Endocrinology, The First People’s Hospital of Chongqing Liang Jiang New Area, Chongqing, ChinaDepartment of Endocrinology, The People’s Hospital of Dazu, Chongqing, ChinaDepartment of Endocrinology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, ChinaDepartment of Endocrinology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, ChinaDepartment of Endocrinology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, ChinaDepartment of Endocrinology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, ChinaThe Key Laboratory of Laboratory Medical Diagnostics in the Ministry of Education and Department of Clinical Biochemistry, College of Laboratory Medicine, Chongqing Medical University, Chongqing, ChinaDepartment of Pediatrics, University of Mississippi Medical Center, Jackson, Mississippi, MS, United StatesDepartment of Endocrinology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, ChinaDepartment of Endocrinology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, ChinaDepartment of Endocrinology, Chongqing Red Cross Hospital (People’s Hospital of Jiangbei District), Chongqing, ChinaDepartment of Endocrinology, Chongqing Red Cross Hospital (People’s Hospital of Jiangbei District), Chongqing, ChinaObjectiveGPHB5 has been found to be associated with glucose and lipid metabolism in animal studies. However, the association of GPHB5 with IR and metabolic disorders remains unknown, and there is a lack of research in humans. Our aim in this study was to investigate the relationship between circulating GPHB5 and metabolic disorders in humans.MethodsBioinformatics analysis was performed to understand the relationship between GPHB5 and metabolic disorders. GPHB5 mRNA expression in mice and rats was determined using RT-qPCR. Circulating GPHB5 concentrations were measured with an ELISA kit. EHC and OGTT were performed in humans.ResultsBioinformatics analysis shows that GPHB5 is associated with metabolic disorders and PCOS. GPHB5 mRNA expression levels in the metabolic-related tissues of HFD-fed mice, db/db and ob/ob mice, and PCOS rats were significantly higher than those of WT mice or rats. In human studies, we find that circulating GPHB5 levels were significantly higher in women with IR and PCOS. GPHB5 levels were positively correlated with age, BMI, WHR, BP, FBG, 2 h-BG, FIns, 2 h-Ins, TC, LDL-C, HbA1c, and FFA, but negatively correlated with adiponectin. Furthermore, GPHB5 was positively correlated with DHEAS and FAI, while negatively correlated with SHBG, FSH, SHBG and FSH. The increased GPHB5 concentration was related to IR and PCOS. After the treatment of metformin, GLP-1RA (Lira), and TZDs, circulating GPHB5 levels were decreased.ConclusionsOur results reveal that circulating GPHB5 could be a biomarker and potential therapeutic target for IR and PCOS in women.https://www.frontiersin.org/articles/10.3389/fendo.2022.1010714/fullGPHB5bioinformaticsinsulin resistancePCOSdrug intervention |
| spellingShingle | Yanping Wang Yanping Wang Ting Xiang Xuyun Xia Hongmin Zhang Shan Geng Gangyi Yang Sheng Qiu Yirui He Rui Liu Ling Li Hua Liu Ke Li Lili Zhang Zerong Liang Jianguo He Elevated circulating GPHB5 levels in women with insulin resistance and polycystic ovary syndrome: A cross-sectional study and multiple intervention studies Frontiers in Endocrinology GPHB5 bioinformatics insulin resistance PCOS drug intervention |
| title | Elevated circulating GPHB5 levels in women with insulin resistance and polycystic ovary syndrome: A cross-sectional study and multiple intervention studies |
| title_full | Elevated circulating GPHB5 levels in women with insulin resistance and polycystic ovary syndrome: A cross-sectional study and multiple intervention studies |
| title_fullStr | Elevated circulating GPHB5 levels in women with insulin resistance and polycystic ovary syndrome: A cross-sectional study and multiple intervention studies |
| title_full_unstemmed | Elevated circulating GPHB5 levels in women with insulin resistance and polycystic ovary syndrome: A cross-sectional study and multiple intervention studies |
| title_short | Elevated circulating GPHB5 levels in women with insulin resistance and polycystic ovary syndrome: A cross-sectional study and multiple intervention studies |
| title_sort | elevated circulating gphb5 levels in women with insulin resistance and polycystic ovary syndrome a cross sectional study and multiple intervention studies |
| topic | GPHB5 bioinformatics insulin resistance PCOS drug intervention |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.1010714/full |
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