Screening of Common Chromosomal Disorders in Iranian Women with Hydatidiform Mole using QF-PCR
Abstract Background: Hydatidiform Mole is a benign trophoblastic tumor is made of ectopic preg-nancy. Abnormalities in the number or structure of chromosomes are causes of Hydatidiform Mole common numerical disorders resulted from proliferating repetitive sequences markers as called STR were studi...
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| Format: | Article |
| Language: | fas |
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Arak Medical University
2016-11-01
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| Series: | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| Subjects: | |
| Online Access: | http://amuj.arakmu.ac.ir/article-1-4477-en.html |
| _version_ | 1818472921705742336 |
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| author | Masoumeh Barari Soyar Sari Ahmad Ebrahimi |
| author_facet | Masoumeh Barari Soyar Sari Ahmad Ebrahimi |
| author_sort | Masoumeh Barari |
| collection | DOAJ |
| description | Abstract
Background: Hydatidiform Mole is a benign trophoblastic tumor is made of ectopic preg-nancy. Abnormalities in the number or structure of chromosomes are causes of Hydatidiform Mole common numerical disorders resulted from proliferating repetitive sequences markers as called STR were studied in the region of chromosome X, Y, 13, 18 and 21. This study aimed to investigate chromosomal disorders prevalent in women with hydatidiform mole, that was performed using QF-PCR techniques.
Materials and Methods: In this study, 50 women with hydatidiform mole and 80 healthy women as controls were selected. For studying the chromosomal abnormalities resulted of proliferating STR, Chromo Quant QF-PCR kit was used. Polymerase chain reaction was performed in PCR machine. Then electrophoresis was performed on Genetic Analyzer. Finally, amplified fragment were analyzed by Gene Marker software Statistical analysis was performed using SPSS version 19, and t-test. Data were expressed as mean ± SD. In this test, p <0.05 represents significant level between two groups.
Results: In this study ،of 50 samples, 8 samples of 47XXY (16%), 40 samples of trisomy 21 (80%) and 2 cases of trisomy 18 (4%) were identified.
Conclusion: Anomalies Trisomy 21 (41 ± 1.58) and 47XXY (9.62 ± 1.36) are significantly associated with mydatidiform mole disease (p <0.001). The highest percentage of samples with tri-somy 21 and 47XXY had the disease. So, it indicates that these anomalies have the highest percentage in the disease. |
| first_indexed | 2024-04-14T04:15:25Z |
| format | Article |
| id | doaj.art-8daf85fa647b45b6852ae8df87c1977f |
| institution | Directory Open Access Journal |
| issn | 1735-5338 2008-644X |
| language | fas |
| last_indexed | 2024-04-14T04:15:25Z |
| publishDate | 2016-11-01 |
| publisher | Arak Medical University |
| record_format | Article |
| series | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| spelling | doaj.art-8daf85fa647b45b6852ae8df87c1977f2022-12-22T02:12:56ZfasArak Medical UniversityMajallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk1735-53382008-644X2016-11-011981625Screening of Common Chromosomal Disorders in Iranian Women with Hydatidiform Mole using QF-PCRMasoumeh Barari0Soyar Sari 1Ahmad Ebrahimi 2Msc Student in Molecular and Cellular Biology, Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad Uni-versity, Tehran, Iran.Assistant Professor, PhD in Molecular and Cellular Biology, Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.Assistant Professor, PhD in Genetics, Research Institute for Endocrine Sciences Metabolism of SMBU, Tehran, Iran.Abstract Background: Hydatidiform Mole is a benign trophoblastic tumor is made of ectopic preg-nancy. Abnormalities in the number or structure of chromosomes are causes of Hydatidiform Mole common numerical disorders resulted from proliferating repetitive sequences markers as called STR were studied in the region of chromosome X, Y, 13, 18 and 21. This study aimed to investigate chromosomal disorders prevalent in women with hydatidiform mole, that was performed using QF-PCR techniques. Materials and Methods: In this study, 50 women with hydatidiform mole and 80 healthy women as controls were selected. For studying the chromosomal abnormalities resulted of proliferating STR, Chromo Quant QF-PCR kit was used. Polymerase chain reaction was performed in PCR machine. Then electrophoresis was performed on Genetic Analyzer. Finally, amplified fragment were analyzed by Gene Marker software Statistical analysis was performed using SPSS version 19, and t-test. Data were expressed as mean ± SD. In this test, p <0.05 represents significant level between two groups. Results: In this study ،of 50 samples, 8 samples of 47XXY (16%), 40 samples of trisomy 21 (80%) and 2 cases of trisomy 18 (4%) were identified. Conclusion: Anomalies Trisomy 21 (41 ± 1.58) and 47XXY (9.62 ± 1.36) are significantly associated with mydatidiform mole disease (p <0.001). The highest percentage of samples with tri-somy 21 and 47XXY had the disease. So, it indicates that these anomalies have the highest percentage in the disease.http://amuj.arakmu.ac.ir/article-1-4477-en.htmlChromosomal disordersHydatidiform moleQF-PCR. |
| spellingShingle | Masoumeh Barari Soyar Sari Ahmad Ebrahimi Screening of Common Chromosomal Disorders in Iranian Women with Hydatidiform Mole using QF-PCR Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk Chromosomal disorders Hydatidiform mole QF-PCR. |
| title | Screening of Common Chromosomal Disorders in Iranian Women with Hydatidiform Mole using QF-PCR |
| title_full | Screening of Common Chromosomal Disorders in Iranian Women with Hydatidiform Mole using QF-PCR |
| title_fullStr | Screening of Common Chromosomal Disorders in Iranian Women with Hydatidiform Mole using QF-PCR |
| title_full_unstemmed | Screening of Common Chromosomal Disorders in Iranian Women with Hydatidiform Mole using QF-PCR |
| title_short | Screening of Common Chromosomal Disorders in Iranian Women with Hydatidiform Mole using QF-PCR |
| title_sort | screening of common chromosomal disorders in iranian women with hydatidiform mole using qf pcr |
| topic | Chromosomal disorders Hydatidiform mole QF-PCR. |
| url | http://amuj.arakmu.ac.ir/article-1-4477-en.html |
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