Additive screening and formula optimization of microbial inhibitor having disease prevention and growth promotion effects on Avena sativa
In order to develop environment friendly microbial inhibitor that can also control disease and promote oat (Avena sativa) growth, the growth rate method and response surface methodology were used to screen wetting agents, preservatives and protective agents at optimal concentrations in this study. A...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1208591/full |
| _version_ | 1797776456858730496 |
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| author | Jiangui Zhang Jiangui Zhang Tuo Yao Tuo Yao Wenlong Gong Yamin Gao Guiqin Zhao Guiqin Zhao |
| author_facet | Jiangui Zhang Jiangui Zhang Tuo Yao Tuo Yao Wenlong Gong Yamin Gao Guiqin Zhao Guiqin Zhao |
| author_sort | Jiangui Zhang |
| collection | DOAJ |
| description | In order to develop environment friendly microbial inhibitor that can also control disease and promote oat (Avena sativa) growth, the growth rate method and response surface methodology were used to screen wetting agents, preservatives and protective agents at optimal concentrations in this study. Antagonistic activity of the tested bacterium and cell-free fermentation liquid against pathogenic fungi was evaluated on potato dextrose agar (PDA) substratum plates by dual culture technique. Oxford cup method was used to measure antagonistic reaction between screened bacteria. According to each screened bacteria with 50 mL were mixed and cultured in Luria-bertani (LB) substratum. Additives of Wetting agents, UV-protectors, and preservatives were screened by single factor test on the growth concentration of screened mixed bacteria. Afterwards, the optimal additives and concentrations were screened by Box-Behnken method. The microbial inhibitor was detected according to national standards GB20287-2006 and tested on oat in a pot experiment. The results showed that: (1) Functional bacteria which including Bacillus velezensis and Brevundimonas faecalis had control effects of 50.00% to 83.29% on three pathogenic fungi, and their cell free-fermentation liquid could inhibit the growth of pathogenic fungi from 23.51% to 39.90%; (2) Tween-80 was most suitable as wetting agents for Mix biocontrol bacteria (MBB) with 1.00% mass fraction; Sorbitol was selected as UV protective agents for MBB with 0.50% mass fraction. And methyl paraben was used as a preservative for MBB, with 0.50% mass fraction; (3) The most effective adjuvant contained 14.96 mL/L Tween-80, 5.12 g/L methylparaben and 5.6 g/L sorbitol; and (4) The microbial inhibitor controlled 45.57% of oat root rot and increased plant height, root length and seedling biomass. This study provides a suitable environment for the protection of mixed biocontrol bacteria, and lays a foundation for the prevention and control of oat diseases, the promotion of growth and the improvement of quality. |
| first_indexed | 2024-03-12T22:50:13Z |
| format | Article |
| id | doaj.art-8db072f6f0be4821a394546ddd82a6f4 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T22:50:13Z |
| publishDate | 2023-07-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-8db072f6f0be4821a394546ddd82a6f42023-07-20T14:29:36ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-07-011410.3389/fmicb.2023.12085911208591Additive screening and formula optimization of microbial inhibitor having disease prevention and growth promotion effects on Avena sativaJiangui Zhang0Jiangui Zhang1Tuo Yao2Tuo Yao3Wenlong Gong4Yamin Gao5Guiqin Zhao6Guiqin Zhao7College of Grassland Science, Gansu Agricultural University, Lanzhou, Gansu, ChinaKey Laboratory of Grassland Ecosystem of Ministry of Education, Lanzhou, Gansu, ChinaCollege of Grassland Science, Gansu Agricultural University, Lanzhou, Gansu, ChinaKey Laboratory of Grassland Ecosystem of Ministry of Education, Lanzhou, Gansu, ChinaCollege of Grassland Science, Gansu Agricultural University, Lanzhou, Gansu, ChinaCollege of Grassland Science, Gansu Agricultural University, Lanzhou, Gansu, ChinaCollege of Grassland Science, Gansu Agricultural University, Lanzhou, Gansu, ChinaKey Laboratory of Grassland Ecosystem of Ministry of Education, Lanzhou, Gansu, ChinaIn order to develop environment friendly microbial inhibitor that can also control disease and promote oat (Avena sativa) growth, the growth rate method and response surface methodology were used to screen wetting agents, preservatives and protective agents at optimal concentrations in this study. Antagonistic activity of the tested bacterium and cell-free fermentation liquid against pathogenic fungi was evaluated on potato dextrose agar (PDA) substratum plates by dual culture technique. Oxford cup method was used to measure antagonistic reaction between screened bacteria. According to each screened bacteria with 50 mL were mixed and cultured in Luria-bertani (LB) substratum. Additives of Wetting agents, UV-protectors, and preservatives were screened by single factor test on the growth concentration of screened mixed bacteria. Afterwards, the optimal additives and concentrations were screened by Box-Behnken method. The microbial inhibitor was detected according to national standards GB20287-2006 and tested on oat in a pot experiment. The results showed that: (1) Functional bacteria which including Bacillus velezensis and Brevundimonas faecalis had control effects of 50.00% to 83.29% on three pathogenic fungi, and their cell free-fermentation liquid could inhibit the growth of pathogenic fungi from 23.51% to 39.90%; (2) Tween-80 was most suitable as wetting agents for Mix biocontrol bacteria (MBB) with 1.00% mass fraction; Sorbitol was selected as UV protective agents for MBB with 0.50% mass fraction. And methyl paraben was used as a preservative for MBB, with 0.50% mass fraction; (3) The most effective adjuvant contained 14.96 mL/L Tween-80, 5.12 g/L methylparaben and 5.6 g/L sorbitol; and (4) The microbial inhibitor controlled 45.57% of oat root rot and increased plant height, root length and seedling biomass. This study provides a suitable environment for the protection of mixed biocontrol bacteria, and lays a foundation for the prevention and control of oat diseases, the promotion of growth and the improvement of quality.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1208591/fullmix biocontrol bacteriaadditive screeningformulation optimizationAvena sativadisease controlgrowth promotion |
| spellingShingle | Jiangui Zhang Jiangui Zhang Tuo Yao Tuo Yao Wenlong Gong Yamin Gao Guiqin Zhao Guiqin Zhao Additive screening and formula optimization of microbial inhibitor having disease prevention and growth promotion effects on Avena sativa Frontiers in Microbiology mix biocontrol bacteria additive screening formulation optimization Avena sativa disease control growth promotion |
| title | Additive screening and formula optimization of microbial inhibitor having disease prevention and growth promotion effects on Avena sativa |
| title_full | Additive screening and formula optimization of microbial inhibitor having disease prevention and growth promotion effects on Avena sativa |
| title_fullStr | Additive screening and formula optimization of microbial inhibitor having disease prevention and growth promotion effects on Avena sativa |
| title_full_unstemmed | Additive screening and formula optimization of microbial inhibitor having disease prevention and growth promotion effects on Avena sativa |
| title_short | Additive screening and formula optimization of microbial inhibitor having disease prevention and growth promotion effects on Avena sativa |
| title_sort | additive screening and formula optimization of microbial inhibitor having disease prevention and growth promotion effects on avena sativa |
| topic | mix biocontrol bacteria additive screening formulation optimization Avena sativa disease control growth promotion |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1208591/full |
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