The Potential of Optimized Liposomes in Enhancement of Cytotoxicity and Apoptosis of Encapsulated Egyptian Propolis on Hep-2 Cell Line
Purpose: Development of pharmaceutical dosage forms of natural products has gained great interest recently. Propolis is a natural product with various active compounds and multiple pharmacological activities. Its resinous nature and low bioavailability were obstacles in the optimum use of this magni...
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MDPI AG
2021-12-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/12/2184 |
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| author | Enas Alaa El-din Abd El-aziz Sherif Farouk Elgayar Fatma M. Mady Mohammed A. S. Abourehab Omiya Ali Hasan Lamis M. Reda Eman Alaaeldin |
| author_facet | Enas Alaa El-din Abd El-aziz Sherif Farouk Elgayar Fatma M. Mady Mohammed A. S. Abourehab Omiya Ali Hasan Lamis M. Reda Eman Alaaeldin |
| author_sort | Enas Alaa El-din Abd El-aziz |
| collection | DOAJ |
| description | Purpose: Development of pharmaceutical dosage forms of natural products has gained great interest recently. Propolis is a natural product with various active compounds and multiple pharmacological activities. Its resinous nature and low bioavailability were obstacles in the optimum use of this magnificent natural product. Aim: This study evaluates the effect of using liposomes as a drug delivery system on the enhancement of the cytotoxic effect of propolis on squamous cell carcinoma cell lines (Hep-2) of head and neck. Methods: An optimized liposomal formulation of propolis was prepared using the conventional thin film hydration method 1, 2. The prepared (Hep-2) cell line was treated with different concentrations of propolis and optimized propolis liposomes for 24 h. The effect of both propolis and propolis liposomes on cell line was investigated using MTT assay, cytological examination, and nuclear morphometric analysis. The effect of the drugs on the cell apoptosis was evaluated using Annexin V. Results: The findings revealed that both propolis and propolis liposomes have a cytotoxic effect on Hep-2 cell line through induction of apoptosis. The effect was dose dependent. However, a statistically significant enhancement in propolis-mediated apoptosis on Hep-2 cells was elucidated due to encapsulation within the prepared liposomes. Conclusion: Liposome is a powerful tool for enhancing the cytotoxicity of propolis against Hep-2 cell line. |
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| id | doaj.art-8db17c82df2742d88ba3525c78322dba |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T03:19:35Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-8db17c82df2742d88ba3525c78322dba2023-11-23T10:07:04ZengMDPI AGPharmaceutics1999-49232021-12-011312218410.3390/pharmaceutics13122184The Potential of Optimized Liposomes in Enhancement of Cytotoxicity and Apoptosis of Encapsulated Egyptian Propolis on Hep-2 Cell LineEnas Alaa El-din Abd El-aziz0Sherif Farouk Elgayar1Fatma M. Mady2Mohammed A. S. Abourehab3Omiya Ali Hasan4Lamis M. Reda5Eman Alaaeldin6Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Minia 61519, EgyptDepartment of Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Minia 61519, EgyptDepartment of Pharmaceutics, Faculty of Pharmacy, Minia University, Minia 61519, EgyptDepartment of Pharmaceutics, Faculty of Pharmacy, Minia University, Minia 61519, EgyptDepartment of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Deraya University, Minia 61768, EgyptDepartment of Clinical Pharmacy, Faculty of Pharmacy, Deraya University, Minia 61768, EgyptDepartment of Pharmaceutics, Faculty of Pharmacy, Minia University, Minia 61519, EgyptPurpose: Development of pharmaceutical dosage forms of natural products has gained great interest recently. Propolis is a natural product with various active compounds and multiple pharmacological activities. Its resinous nature and low bioavailability were obstacles in the optimum use of this magnificent natural product. Aim: This study evaluates the effect of using liposomes as a drug delivery system on the enhancement of the cytotoxic effect of propolis on squamous cell carcinoma cell lines (Hep-2) of head and neck. Methods: An optimized liposomal formulation of propolis was prepared using the conventional thin film hydration method 1, 2. The prepared (Hep-2) cell line was treated with different concentrations of propolis and optimized propolis liposomes for 24 h. The effect of both propolis and propolis liposomes on cell line was investigated using MTT assay, cytological examination, and nuclear morphometric analysis. The effect of the drugs on the cell apoptosis was evaluated using Annexin V. Results: The findings revealed that both propolis and propolis liposomes have a cytotoxic effect on Hep-2 cell line through induction of apoptosis. The effect was dose dependent. However, a statistically significant enhancement in propolis-mediated apoptosis on Hep-2 cells was elucidated due to encapsulation within the prepared liposomes. Conclusion: Liposome is a powerful tool for enhancing the cytotoxicity of propolis against Hep-2 cell line.https://www.mdpi.com/1999-4923/13/12/2184propolisliposomesHep-2apoptosiscytotoxicity |
| spellingShingle | Enas Alaa El-din Abd El-aziz Sherif Farouk Elgayar Fatma M. Mady Mohammed A. S. Abourehab Omiya Ali Hasan Lamis M. Reda Eman Alaaeldin The Potential of Optimized Liposomes in Enhancement of Cytotoxicity and Apoptosis of Encapsulated Egyptian Propolis on Hep-2 Cell Line Pharmaceutics propolis liposomes Hep-2 apoptosis cytotoxicity |
| title | The Potential of Optimized Liposomes in Enhancement of Cytotoxicity and Apoptosis of Encapsulated Egyptian Propolis on Hep-2 Cell Line |
| title_full | The Potential of Optimized Liposomes in Enhancement of Cytotoxicity and Apoptosis of Encapsulated Egyptian Propolis on Hep-2 Cell Line |
| title_fullStr | The Potential of Optimized Liposomes in Enhancement of Cytotoxicity and Apoptosis of Encapsulated Egyptian Propolis on Hep-2 Cell Line |
| title_full_unstemmed | The Potential of Optimized Liposomes in Enhancement of Cytotoxicity and Apoptosis of Encapsulated Egyptian Propolis on Hep-2 Cell Line |
| title_short | The Potential of Optimized Liposomes in Enhancement of Cytotoxicity and Apoptosis of Encapsulated Egyptian Propolis on Hep-2 Cell Line |
| title_sort | potential of optimized liposomes in enhancement of cytotoxicity and apoptosis of encapsulated egyptian propolis on hep 2 cell line |
| topic | propolis liposomes Hep-2 apoptosis cytotoxicity |
| url | https://www.mdpi.com/1999-4923/13/12/2184 |
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