Tectorigenin alleviates the apoptosis and inflammation in spinal cord injury cell model through inhibiting insulin-like growth factor-binding protein 6
Since tectorigenin has been reported to possess anti-inflammation, redox balance restoration, and anti-apoptosis properties, we determine to unravel whether tectorigenin has potential in alleviating spinal cord injury (SCI). Herein, PC12 cells were induced by lipopolysaccharide (LPS) to establish in...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2023-04-01
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| Series: | Open Medicine |
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| Online Access: | https://doi.org/10.1515/med-2023-0680 |
| _version_ | 1797832502934503424 |
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| author | Zhou Liqiang Yan Kui Xing Shuxing Cheng Jun |
| author_facet | Zhou Liqiang Yan Kui Xing Shuxing Cheng Jun |
| author_sort | Zhou Liqiang |
| collection | DOAJ |
| description | Since tectorigenin has been reported to possess anti-inflammation, redox balance restoration, and anti-apoptosis properties, we determine to unravel whether tectorigenin has potential in alleviating spinal cord injury (SCI). Herein, PC12 cells were induced by lipopolysaccharide (LPS) to establish in vitro SCI models. The cell viability and apoptosis were detected through cell counting kit-8 and flow cytometry assays. The caspase-3/8/9 content was measured by colorimetric method. Western blot was conducted to quantify the expressions of cleaved caspse-3/8/9, IGFBP6, TLR4, IκBα, p-IκBα, RELA proto-oncogene, p65, and p-p65. Enzyme-linked immunosorbent assay and real-time quantitative polymerase chain reaction were carried out to quantitate expressions of IGFBP6, interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α). SwissTargetPrediction and GSE21497 database were utilized to predict the potential therapeutic targets of tectorigenin. Comparison of IGFBP6 expression in SCI tissues and normal tissues was analyzed by GEO2R. Our study found that LPS induced the declined cell viability, elevated cell apoptosis, upregulation of caspase-3/8/9, cleaved caspase-3/8/9, IL-1β, IL-6, TNF-α, IGFBP6, and TLR4, and the activation of IκBα and p65 in PC12 cells. Tectorigenin reversed the above effects of LPS. IGFBP6 was predicted to be the potential therapeutic target of tectorigenin and was overexpressed in SCI tissues. Notably, IGFBP6 overexpression offset the effects of tectorigenin on PC12 cells. In conclusion, tectorigenin could alleviate the LPS-induced apoptosis, inflammation, and activation of NF-κB signaling in SCI cell models via inhibiting IGFBP6. |
| first_indexed | 2024-04-09T14:10:00Z |
| format | Article |
| id | doaj.art-8db2b758c633431b8c864619dfc27554 |
| institution | Directory Open Access Journal |
| issn | 2391-5463 |
| language | English |
| last_indexed | 2024-04-09T14:10:00Z |
| publishDate | 2023-04-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Medicine |
| spelling | doaj.art-8db2b758c633431b8c864619dfc275542023-05-06T15:31:53ZengDe GruyterOpen Medicine2391-54632023-04-01181355610.1515/med-2023-0680Tectorigenin alleviates the apoptosis and inflammation in spinal cord injury cell model through inhibiting insulin-like growth factor-binding protein 6Zhou Liqiang0Yan Kui1Xing Shuxing2Cheng Jun3Department of Orthopedics, Chengdu Fifth People’s Hospital, Chengdu, Sichuan Province, 611130, ChinaDepartment of Orthopedics, Chengdu Fifth People’s Hospital, Chengdu, Sichuan Province, 611130, ChinaDepartment of Orthopedics, Chengdu Fifth People’s Hospital, No. 33 Mashi Street, Wenjiang District, Chengdu, Sichuan Province, 611130, ChinaDepartment of Orthopedics, Chengdu Fifth People’s Hospital, Chengdu, Sichuan Province, 611130, ChinaSince tectorigenin has been reported to possess anti-inflammation, redox balance restoration, and anti-apoptosis properties, we determine to unravel whether tectorigenin has potential in alleviating spinal cord injury (SCI). Herein, PC12 cells were induced by lipopolysaccharide (LPS) to establish in vitro SCI models. The cell viability and apoptosis were detected through cell counting kit-8 and flow cytometry assays. The caspase-3/8/9 content was measured by colorimetric method. Western blot was conducted to quantify the expressions of cleaved caspse-3/8/9, IGFBP6, TLR4, IκBα, p-IκBα, RELA proto-oncogene, p65, and p-p65. Enzyme-linked immunosorbent assay and real-time quantitative polymerase chain reaction were carried out to quantitate expressions of IGFBP6, interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α). SwissTargetPrediction and GSE21497 database were utilized to predict the potential therapeutic targets of tectorigenin. Comparison of IGFBP6 expression in SCI tissues and normal tissues was analyzed by GEO2R. Our study found that LPS induced the declined cell viability, elevated cell apoptosis, upregulation of caspase-3/8/9, cleaved caspase-3/8/9, IL-1β, IL-6, TNF-α, IGFBP6, and TLR4, and the activation of IκBα and p65 in PC12 cells. Tectorigenin reversed the above effects of LPS. IGFBP6 was predicted to be the potential therapeutic target of tectorigenin and was overexpressed in SCI tissues. Notably, IGFBP6 overexpression offset the effects of tectorigenin on PC12 cells. In conclusion, tectorigenin could alleviate the LPS-induced apoptosis, inflammation, and activation of NF-κB signaling in SCI cell models via inhibiting IGFBP6.https://doi.org/10.1515/med-2023-0680spinal cord injurypc12lpstectorigeninigfbp6 |
| spellingShingle | Zhou Liqiang Yan Kui Xing Shuxing Cheng Jun Tectorigenin alleviates the apoptosis and inflammation in spinal cord injury cell model through inhibiting insulin-like growth factor-binding protein 6 Open Medicine spinal cord injury pc12 lps tectorigenin igfbp6 |
| title | Tectorigenin alleviates the apoptosis and inflammation in spinal cord injury cell model through inhibiting insulin-like growth factor-binding protein 6 |
| title_full | Tectorigenin alleviates the apoptosis and inflammation in spinal cord injury cell model through inhibiting insulin-like growth factor-binding protein 6 |
| title_fullStr | Tectorigenin alleviates the apoptosis and inflammation in spinal cord injury cell model through inhibiting insulin-like growth factor-binding protein 6 |
| title_full_unstemmed | Tectorigenin alleviates the apoptosis and inflammation in spinal cord injury cell model through inhibiting insulin-like growth factor-binding protein 6 |
| title_short | Tectorigenin alleviates the apoptosis and inflammation in spinal cord injury cell model through inhibiting insulin-like growth factor-binding protein 6 |
| title_sort | tectorigenin alleviates the apoptosis and inflammation in spinal cord injury cell model through inhibiting insulin like growth factor binding protein 6 |
| topic | spinal cord injury pc12 lps tectorigenin igfbp6 |
| url | https://doi.org/10.1515/med-2023-0680 |
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