High-content live-cell multiplex screen for chemogenomic compound annotation based on nuclear morphology
Summary: Well-characterized small molecules enable the study of cell processes and facilitate target validation. Here, we describe a high-content multiplex screen to investigate cell viability over 48 h, which can be combined with investigating phenotypic features, such as tubulin binding and mitoch...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722006712 |
| _version_ | 1811183868863053824 |
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| author | Amelie Tjaden Robert T. Giessmann Stefan Knapp Martin Schröder Susanne Müller |
| author_facet | Amelie Tjaden Robert T. Giessmann Stefan Knapp Martin Schröder Susanne Müller |
| author_sort | Amelie Tjaden |
| collection | DOAJ |
| description | Summary: Well-characterized small molecules enable the study of cell processes and facilitate target validation. Here, we describe a high-content multiplex screen to investigate cell viability over 48 h, which can be combined with investigating phenotypic features, such as tubulin binding and mitochondrial content, as initial cellular quality control of diverse compounds. The protocol is on a live-cell basis and easily adaptable and scalable. It details cell preparation, compound handling, plate layout configuration, image acquisition with the CQ1, and data analysis using the CellPathfinder software.For complete details on the use and execution of this protocol, please refer to Tjaden et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-11T13:03:55Z |
| format | Article |
| id | doaj.art-8db359038ed543c5b39ff8c128c474e7 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-11T13:03:55Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-8db359038ed543c5b39ff8c128c474e72022-12-22T04:22:50ZengElsevierSTAR Protocols2666-16672022-12-0134101791High-content live-cell multiplex screen for chemogenomic compound annotation based on nuclear morphologyAmelie Tjaden0Robert T. Giessmann1Stefan Knapp2Martin Schröder3Susanne Müller4Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Max-von-Laue-Str.9, 60438 Frankfurt, Germany; Structural Genomics Consortium, BMLS, Goethe University Frankfurt, Max-von-Laue-Str. 15, 60438 Frankfurt, GermanyBayer AG, Research & Development, Pharmaceuticals, 13353 Berlin, Germany; Institute for Globally Distributed Open Research and Education (IGDORE), Berlin, GermanyInstitute of Pharmaceutical Chemistry, Goethe University Frankfurt, Max-von-Laue-Str.9, 60438 Frankfurt, Germany; Structural Genomics Consortium, BMLS, Goethe University Frankfurt, Max-von-Laue-Str. 15, 60438 Frankfurt, GermanyInstitute of Pharmaceutical Chemistry, Goethe University Frankfurt, Max-von-Laue-Str.9, 60438 Frankfurt, Germany; Structural Genomics Consortium, BMLS, Goethe University Frankfurt, Max-von-Laue-Str. 15, 60438 Frankfurt, Germany; Corresponding authorInstitute of Pharmaceutical Chemistry, Goethe University Frankfurt, Max-von-Laue-Str.9, 60438 Frankfurt, Germany; Structural Genomics Consortium, BMLS, Goethe University Frankfurt, Max-von-Laue-Str. 15, 60438 Frankfurt, Germany; Corresponding authorSummary: Well-characterized small molecules enable the study of cell processes and facilitate target validation. Here, we describe a high-content multiplex screen to investigate cell viability over 48 h, which can be combined with investigating phenotypic features, such as tubulin binding and mitochondrial content, as initial cellular quality control of diverse compounds. The protocol is on a live-cell basis and easily adaptable and scalable. It details cell preparation, compound handling, plate layout configuration, image acquisition with the CQ1, and data analysis using the CellPathfinder software.For complete details on the use and execution of this protocol, please refer to Tjaden et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722006712Cell biologyCell-based assaysCancerHigh throughput screeningMicroscopy |
| spellingShingle | Amelie Tjaden Robert T. Giessmann Stefan Knapp Martin Schröder Susanne Müller High-content live-cell multiplex screen for chemogenomic compound annotation based on nuclear morphology STAR Protocols Cell biology Cell-based assays Cancer High throughput screening Microscopy |
| title | High-content live-cell multiplex screen for chemogenomic compound annotation based on nuclear morphology |
| title_full | High-content live-cell multiplex screen for chemogenomic compound annotation based on nuclear morphology |
| title_fullStr | High-content live-cell multiplex screen for chemogenomic compound annotation based on nuclear morphology |
| title_full_unstemmed | High-content live-cell multiplex screen for chemogenomic compound annotation based on nuclear morphology |
| title_short | High-content live-cell multiplex screen for chemogenomic compound annotation based on nuclear morphology |
| title_sort | high content live cell multiplex screen for chemogenomic compound annotation based on nuclear morphology |
| topic | Cell biology Cell-based assays Cancer High throughput screening Microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722006712 |
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