Molecular and Immune Characteristics for Lung Adenocarcinoma Patients With ERLIN2 Overexpression
BackgroundEndoplasmic reticulum lipid raft-associated protein 2 (ERLIN2) is protein contained in the membrane of the endoplasmic reticulum. In lung adenocarcinoma (LUAD), the molecular function of ERLIN2 and the correlation between ERLIN2 and tumor-infiltrating immune cells have been unclear. The ai...
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| Format: | Article |
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Frontiers Media S.A.
2020-12-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2020.568440/full |
| _version_ | 1818854768685088768 |
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| author | Yifei Liu Yifei Liu Pengfei Xie Daishang Jiang Jian Liu Jianguo Zhang Tingting Bian Jiahai Shi |
| author_facet | Yifei Liu Yifei Liu Pengfei Xie Daishang Jiang Jian Liu Jianguo Zhang Tingting Bian Jiahai Shi |
| author_sort | Yifei Liu |
| collection | DOAJ |
| description | BackgroundEndoplasmic reticulum lipid raft-associated protein 2 (ERLIN2) is protein contained in the membrane of the endoplasmic reticulum. In lung adenocarcinoma (LUAD), the molecular function of ERLIN2 and the correlation between ERLIN2 and tumor-infiltrating immune cells have been unclear. The aim of our study was to determine the role of ERLIN2 in LUAD development to provide a better understanding of the molecular pathogenesis of this disease and identify new therapeutic targets for its treatment.MethodsImmunohistochemistry, Western blotting, and real-time quantitative polymerase chain reaction were used to detect protein and mRNA levels of ERLIN2 in LUAD and adjacent normal tissues. Using the A549, H1299 cell line, ERLIN2-short hairpin RNA was applied to silence ERLIN2 to determine its role in LUAD cell proliferation and invasion. Based on mRNA expression of ERLIN2 from the Cancer Genome Atlas (TCGA) database, we identified ERLIN2-related protein-coding genes and analyzed the Kyoto Encyclopedia of Genes and Genomes pathway to explore its potential biological functions and determined the correlation between ERLIN2 and tumor-infiltrating immune cells.ResultsERLIN2 was abnormally expressed in a variety of tumor tissues and is highly expressed in LUAD. This overexpression was associated with histological grade (P = 0.044), TNM stage (P = 0.01), and lymph node metastasis (P = 0.038). Patient overall survival was poorer with ERLIN2 overexpression. Downregulation of ERLIN2 inhibited LUAD cell proliferation and invasion in vitro. Based on mRNA expression of ERLIN2 from the TCGA database, 13 ERLIN2-related genes and 10 pathways were identified and showed a correlation between ERLIN2 and naive B cells and neutrophils.ConclusionERLIN2 could serve as a potential diagnostic and prognostic biomarker for LUAD and has demonstrated to be correlated with immune infiltrates, which suggests that it may represent a new therapeutic target for LUAD. |
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| id | doaj.art-8db84a3c7ed949fa9cf5b6e5a8439bdb |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-19T07:57:58Z |
| publishDate | 2020-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj.art-8db84a3c7ed949fa9cf5b6e5a8439bdb2022-12-21T20:29:57ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-12-011110.3389/fimmu.2020.568440568440Molecular and Immune Characteristics for Lung Adenocarcinoma Patients With ERLIN2 OverexpressionYifei Liu0Yifei Liu1Pengfei Xie2Daishang Jiang3Jian Liu4Jianguo Zhang5Tingting Bian6Jiahai Shi7Department of Pathology, Affiliated Hospital of Nantong University, Nantong, ChinaMedical School of Nantong University, Nantong, ChinaDepartment of Thoracic Surgery, Affiliated Tumor Hospital of Nantong University, Nantong, ChinaDepartment of Emergency Medicine, Affiliated Hospital of Nantong University, Nantong, ChinaDepartment of Chemotherapy, Affiliated Hospital of Nantong University, Nantong, ChinaDepartment of Pathology, Affiliated Hospital of Nantong University, Nantong, ChinaDepartment of Pathology, Affiliated Hospital of Nantong University, Nantong, ChinaDepartments of Cardio-Thoracic Surgery, Affiliated Hospital of Nantong University, Nantong, ChinaBackgroundEndoplasmic reticulum lipid raft-associated protein 2 (ERLIN2) is protein contained in the membrane of the endoplasmic reticulum. In lung adenocarcinoma (LUAD), the molecular function of ERLIN2 and the correlation between ERLIN2 and tumor-infiltrating immune cells have been unclear. The aim of our study was to determine the role of ERLIN2 in LUAD development to provide a better understanding of the molecular pathogenesis of this disease and identify new therapeutic targets for its treatment.MethodsImmunohistochemistry, Western blotting, and real-time quantitative polymerase chain reaction were used to detect protein and mRNA levels of ERLIN2 in LUAD and adjacent normal tissues. Using the A549, H1299 cell line, ERLIN2-short hairpin RNA was applied to silence ERLIN2 to determine its role in LUAD cell proliferation and invasion. Based on mRNA expression of ERLIN2 from the Cancer Genome Atlas (TCGA) database, we identified ERLIN2-related protein-coding genes and analyzed the Kyoto Encyclopedia of Genes and Genomes pathway to explore its potential biological functions and determined the correlation between ERLIN2 and tumor-infiltrating immune cells.ResultsERLIN2 was abnormally expressed in a variety of tumor tissues and is highly expressed in LUAD. This overexpression was associated with histological grade (P = 0.044), TNM stage (P = 0.01), and lymph node metastasis (P = 0.038). Patient overall survival was poorer with ERLIN2 overexpression. Downregulation of ERLIN2 inhibited LUAD cell proliferation and invasion in vitro. Based on mRNA expression of ERLIN2 from the TCGA database, 13 ERLIN2-related genes and 10 pathways were identified and showed a correlation between ERLIN2 and naive B cells and neutrophils.ConclusionERLIN2 could serve as a potential diagnostic and prognostic biomarker for LUAD and has demonstrated to be correlated with immune infiltrates, which suggests that it may represent a new therapeutic target for LUAD.https://www.frontiersin.org/articles/10.3389/fimmu.2020.568440/fullERLIN2lung adenocarcinomaprognosissurvivaltumor-infiltrating immune cells |
| spellingShingle | Yifei Liu Yifei Liu Pengfei Xie Daishang Jiang Jian Liu Jianguo Zhang Tingting Bian Jiahai Shi Molecular and Immune Characteristics for Lung Adenocarcinoma Patients With ERLIN2 Overexpression Frontiers in Immunology ERLIN2 lung adenocarcinoma prognosis survival tumor-infiltrating immune cells |
| title | Molecular and Immune Characteristics for Lung Adenocarcinoma Patients With ERLIN2 Overexpression |
| title_full | Molecular and Immune Characteristics for Lung Adenocarcinoma Patients With ERLIN2 Overexpression |
| title_fullStr | Molecular and Immune Characteristics for Lung Adenocarcinoma Patients With ERLIN2 Overexpression |
| title_full_unstemmed | Molecular and Immune Characteristics for Lung Adenocarcinoma Patients With ERLIN2 Overexpression |
| title_short | Molecular and Immune Characteristics for Lung Adenocarcinoma Patients With ERLIN2 Overexpression |
| title_sort | molecular and immune characteristics for lung adenocarcinoma patients with erlin2 overexpression |
| topic | ERLIN2 lung adenocarcinoma prognosis survival tumor-infiltrating immune cells |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2020.568440/full |
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