Bioprospecting of Ureolytic Bacteria From Laguna Salada for Biomineralization Applications
The processes of biomineralization, mediated by ureolytic bacteria, possess a wide range of technological applications, such as the formation of biocements and remediation of water and soil environments. For this reason, the bioprospecting of new ureolytic bacteria is interesting for its application...
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Frontiers Media S.A.
2019-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2018.00209/full |
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| author | Dayana Arias Dayana Arias Luis A. Cisternas Luis A. Cisternas Carol Miranda Mariella Rivas Mariella Rivas |
| author_facet | Dayana Arias Dayana Arias Luis A. Cisternas Luis A. Cisternas Carol Miranda Mariella Rivas Mariella Rivas |
| author_sort | Dayana Arias |
| collection | DOAJ |
| description | The processes of biomineralization, mediated by ureolytic bacteria, possess a wide range of technological applications, such as the formation of biocements and remediation of water and soil environments. For this reason, the bioprospecting of new ureolytic bacteria is interesting for its application to these technologies, particularly for water treatment. This study demonstrates the isolation, selection, and identification of halotolerant ureolytic bacteria from Laguna Salada (inland from Atacama Desert) and the evaluation of their ability to precipitate calcium carbonate crystals in freshwater in the presence of calcium ions, as well as the ability to induce the precipitation of crystals from different ions present in seawater. Twenty-four halotolerant ureolytic bacteria whose molecular identification gives between 99 and 100% identity with species of the genus Bacillus, Porphyrobacter, Pseudomonas, Salinivibrio, and Halomonas were isolated. When cultivated in freshwater, urea, and calcium chloride, all species are able to biomineralize calcium carbonate in different concentrations. In seawater, the strains that biomineralize the highest concentration of calcium carbonate correspond to Bacillus subtilis and Halomonas sp. SEM–EDX and XRD analyses determined that both bacteria induce the formation of 9–33% halite (NaCl), 31–66% monohydrocalcite (CaCO3 × H2O), and 24–27% struvite (MgNH4PO4 × 6H2O). Additionally, B.subtilis induces the formation of 7% anhydrite (CaSO4). In seawater, B.subtilis and Halomonas sp. were able to precipitate both calcium (96–97%) and magnesium (63–67%) ions over 14 days of testing. Ion removal assays with B.subtilis immobilized in beads indicate a direct relationship between the urea concentration and a greater removal of ions with similar rates to free cells. These results demonstrate that the biomineralization mediated by bacterial urea hydrolysis is feasible in both freshwater and seawater, and we propose its application as a new technology in improving water quality for industrial uses. |
| first_indexed | 2024-12-23T05:28:11Z |
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| id | doaj.art-8db9199ad2754b9790c79a545345beb3 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-23T05:28:11Z |
| publishDate | 2019-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-8db9199ad2754b9790c79a545345beb32022-12-21T17:58:33ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852019-01-01610.3389/fbioe.2018.00209420778Bioprospecting of Ureolytic Bacteria From Laguna Salada for Biomineralization ApplicationsDayana Arias0Dayana Arias1Luis A. Cisternas2Luis A. Cisternas3Carol Miranda4Mariella Rivas5Mariella Rivas6Departamento de Ingeniería Química y Procesos de Minerales, Universidad de Antofagasta, Antofagasta, ChileLaboratorio de Biotecnología Algal y Sustentabilidad (BIOAL), Departamento de Biotecnología, Facultad de Ciencias del Mar y Recursos Biológicos, Universidad de Antofagasta, Antofagasta, ChileDepartamento de Ingeniería Química y Procesos de Minerales, Universidad de Antofagasta, Antofagasta, ChileCentro de Investigación Científico Tecnológico Para la Minería, Antofagasta, ChileLaboratorio de Biotecnología Algal y Sustentabilidad (BIOAL), Departamento de Biotecnología, Facultad de Ciencias del Mar y Recursos Biológicos, Universidad de Antofagasta, Antofagasta, ChileLaboratorio de Biotecnología Algal y Sustentabilidad (BIOAL), Departamento de Biotecnología, Facultad de Ciencias del Mar y Recursos Biológicos, Universidad de Antofagasta, Antofagasta, ChileCentro de Investigación Científico Tecnológico Para la Minería, Antofagasta, ChileThe processes of biomineralization, mediated by ureolytic bacteria, possess a wide range of technological applications, such as the formation of biocements and remediation of water and soil environments. For this reason, the bioprospecting of new ureolytic bacteria is interesting for its application to these technologies, particularly for water treatment. This study demonstrates the isolation, selection, and identification of halotolerant ureolytic bacteria from Laguna Salada (inland from Atacama Desert) and the evaluation of their ability to precipitate calcium carbonate crystals in freshwater in the presence of calcium ions, as well as the ability to induce the precipitation of crystals from different ions present in seawater. Twenty-four halotolerant ureolytic bacteria whose molecular identification gives between 99 and 100% identity with species of the genus Bacillus, Porphyrobacter, Pseudomonas, Salinivibrio, and Halomonas were isolated. When cultivated in freshwater, urea, and calcium chloride, all species are able to biomineralize calcium carbonate in different concentrations. In seawater, the strains that biomineralize the highest concentration of calcium carbonate correspond to Bacillus subtilis and Halomonas sp. SEM–EDX and XRD analyses determined that both bacteria induce the formation of 9–33% halite (NaCl), 31–66% monohydrocalcite (CaCO3 × H2O), and 24–27% struvite (MgNH4PO4 × 6H2O). Additionally, B.subtilis induces the formation of 7% anhydrite (CaSO4). In seawater, B.subtilis and Halomonas sp. were able to precipitate both calcium (96–97%) and magnesium (63–67%) ions over 14 days of testing. Ion removal assays with B.subtilis immobilized in beads indicate a direct relationship between the urea concentration and a greater removal of ions with similar rates to free cells. These results demonstrate that the biomineralization mediated by bacterial urea hydrolysis is feasible in both freshwater and seawater, and we propose its application as a new technology in improving water quality for industrial uses.https://www.frontiersin.org/article/10.3389/fbioe.2018.00209/fullbiomineralizationureolytic bacteriastruvitemonohydrocalcitebioprospectingurease |
| spellingShingle | Dayana Arias Dayana Arias Luis A. Cisternas Luis A. Cisternas Carol Miranda Mariella Rivas Mariella Rivas Bioprospecting of Ureolytic Bacteria From Laguna Salada for Biomineralization Applications Frontiers in Bioengineering and Biotechnology biomineralization ureolytic bacteria struvite monohydrocalcite bioprospecting urease |
| title | Bioprospecting of Ureolytic Bacteria From Laguna Salada for Biomineralization Applications |
| title_full | Bioprospecting of Ureolytic Bacteria From Laguna Salada for Biomineralization Applications |
| title_fullStr | Bioprospecting of Ureolytic Bacteria From Laguna Salada for Biomineralization Applications |
| title_full_unstemmed | Bioprospecting of Ureolytic Bacteria From Laguna Salada for Biomineralization Applications |
| title_short | Bioprospecting of Ureolytic Bacteria From Laguna Salada for Biomineralization Applications |
| title_sort | bioprospecting of ureolytic bacteria from laguna salada for biomineralization applications |
| topic | biomineralization ureolytic bacteria struvite monohydrocalcite bioprospecting urease |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2018.00209/full |
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