Genotypic Frequency of RHC Antigen in Women of Reproductive Age in Pakistan
Objective: To determine the genotypic frequency of RHC antigen in females of reproductive age. Study Design: Cross-sectional study. Place and Duration of Study: Armed Forces Institute of Transfusion (AFIT) and Pak Emirates Military Hospital, (PEMH) in collaboration with NUMS Pakistan, from De...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Army Medical College Rawalpindi
2022-11-01
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| Series: | Pakistan Armed Forces Medical Journal |
| Subjects: | |
| Online Access: | https://www.pafmj.org/index.php/PAFMJ/article/view/8470 |
| _version_ | 1811186556800598016 |
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| author | Saima Bashir Fahim Akhtar Mohammad Abdul Naeem Maria Khan Sunila Tashfeen Saleem Ahmed Khan |
| author_facet | Saima Bashir Fahim Akhtar Mohammad Abdul Naeem Maria Khan Sunila Tashfeen Saleem Ahmed Khan |
| author_sort | Saima Bashir |
| collection | DOAJ |
| description |
Objective: To determine the genotypic frequency of RHC antigen in females of reproductive age.
Study Design: Cross-sectional study.
Place and Duration of Study: Armed Forces Institute of Transfusion (AFIT) and Pak Emirates Military Hospital, (PEMH) in collaboration with NUMS Pakistan, from Dec 2020 to Dec 2021.
Methodology: Females aged 16 to 45 years were recruited in the study. Demographic data, including age, parity, ethnicity etc.,was recorded on a proforma. Venous blood samples were collected in EDTA tubes, and DNA was extracted using 5% Chelex TM. A conventional Polymerase chain reaction and amplified products were subjected to Polyacrylamide Gel electrophoresis.
Results: Amongst 200 females, 172(86%) had the expression of RHC antigen, while 28(14%) were negative for the respective antigen. These results were valuable in predicting the risk of hemolytic disease in fetuses and newborns and alloimmunization in our population.
Conclusion: Females in the reproductive age group had RHC positivity of 86%. This data will help predict the risk of HDFN in future pregnancies and individuals at risk of alloimmunization in RHC-negative women.
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| first_indexed | 2024-04-11T13:48:34Z |
| format | Article |
| id | doaj.art-8dbb8166b55b405681679661c063c275 |
| institution | Directory Open Access Journal |
| issn | 0030-9648 2411-8842 |
| language | English |
| last_indexed | 2024-04-11T13:48:34Z |
| publishDate | 2022-11-01 |
| publisher | Army Medical College Rawalpindi |
| record_format | Article |
| series | Pakistan Armed Forces Medical Journal |
| spelling | doaj.art-8dbb8166b55b405681679661c063c2752022-12-22T04:20:53ZengArmy Medical College RawalpindiPakistan Armed Forces Medical Journal0030-96482411-88422022-11-0172510.51253/pafmj.v72i5.8470Genotypic Frequency of RHC Antigen in Women of Reproductive Age in PakistanSaima Bashir0Fahim Akhtar1Mohammad Abdul Naeem2Maria Khan3Sunila Tashfeen4Saleem Ahmed Khan5Army Medical College/National University of Medical Science (NUMS) Rawalpindi PakistanPak Emirates Military Hospital/National University Medical Science (NUMS) Rawalpindi PakistanCombined Military Hospital, Lahore/National University of Medical Science (NUMS) PakistanArmed Forces Institute of Transfusion/National University of Medical Science (NUMS) Rawalpindi PakistanPak Emirates Military Hospital/National University Medical Science (NUMS) Rawalpindi PakistanNational University of Medical Science (NUMS) Rawalpindi Pakistan Objective: To determine the genotypic frequency of RHC antigen in females of reproductive age. Study Design: Cross-sectional study. Place and Duration of Study: Armed Forces Institute of Transfusion (AFIT) and Pak Emirates Military Hospital, (PEMH) in collaboration with NUMS Pakistan, from Dec 2020 to Dec 2021. Methodology: Females aged 16 to 45 years were recruited in the study. Demographic data, including age, parity, ethnicity etc.,was recorded on a proforma. Venous blood samples were collected in EDTA tubes, and DNA was extracted using 5% Chelex TM. A conventional Polymerase chain reaction and amplified products were subjected to Polyacrylamide Gel electrophoresis. Results: Amongst 200 females, 172(86%) had the expression of RHC antigen, while 28(14%) were negative for the respective antigen. These results were valuable in predicting the risk of hemolytic disease in fetuses and newborns and alloimmunization in our population. Conclusion: Females in the reproductive age group had RHC positivity of 86%. This data will help predict the risk of HDFN in future pregnancies and individuals at risk of alloimmunization in RHC-negative women. https://www.pafmj.org/index.php/PAFMJ/article/view/8470AlloimmunizationGenotypingHemolytic disease of the fetus and newborn (HDFN)Polymerase chain reaction (PCR) |
| spellingShingle | Saima Bashir Fahim Akhtar Mohammad Abdul Naeem Maria Khan Sunila Tashfeen Saleem Ahmed Khan Genotypic Frequency of RHC Antigen in Women of Reproductive Age in Pakistan Pakistan Armed Forces Medical Journal Alloimmunization Genotyping Hemolytic disease of the fetus and newborn (HDFN) Polymerase chain reaction (PCR) |
| title | Genotypic Frequency of RHC Antigen in Women of Reproductive Age in Pakistan |
| title_full | Genotypic Frequency of RHC Antigen in Women of Reproductive Age in Pakistan |
| title_fullStr | Genotypic Frequency of RHC Antigen in Women of Reproductive Age in Pakistan |
| title_full_unstemmed | Genotypic Frequency of RHC Antigen in Women of Reproductive Age in Pakistan |
| title_short | Genotypic Frequency of RHC Antigen in Women of Reproductive Age in Pakistan |
| title_sort | genotypic frequency of rhc antigen in women of reproductive age in pakistan |
| topic | Alloimmunization Genotyping Hemolytic disease of the fetus and newborn (HDFN) Polymerase chain reaction (PCR) |
| url | https://www.pafmj.org/index.php/PAFMJ/article/view/8470 |
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