Huoluo Xiaoling Pellet promotes microglia M2 polarization through increasing MCPIP1 expression for ischemia stroke alleviation
Huoluo Xiaoling Pellet (HXP), a Chinese patent medicine, is commonly administered for the treatment of treat ischemic strokes. MCPIP1, an inducible suppressor of the inflammatory response, is a regulator of microglial M2 polarization. This study aimed to explore whether HXP can promote microglial M2...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Biomedicine & Pharmacotherapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332223007047 |
| _version_ | 1797801855329239040 |
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| author | Wei Shen Xiaoguang Wang Meiqi Tang Lan Yao Chenyu Wan Jianli Niu Pappachan E. Kolattukudy Zhuqing Jin |
| author_facet | Wei Shen Xiaoguang Wang Meiqi Tang Lan Yao Chenyu Wan Jianli Niu Pappachan E. Kolattukudy Zhuqing Jin |
| author_sort | Wei Shen |
| collection | DOAJ |
| description | Huoluo Xiaoling Pellet (HXP), a Chinese patent medicine, is commonly administered for the treatment of treat ischemic strokes. MCPIP1, an inducible suppressor of the inflammatory response, is a regulator of microglial M2 polarization. This study aimed to explore whether HXP can promote microglial M2 polarization by upregulating MCPIP1 expression, consequently mitigating cerebral ischemic injury. Our study involved 85 Sprague-Dawley rats (weighing 250–280 g). We established middle cerebral artery occlusion (MCAO) and oxygen-glucose deprivation-reoxygenation (OGD/R) models with MCPIP1 knockdown to assess the effects of HXP on ischemic strokes. Our findings show that HXP reduced brain water content, improved neurological function, and inhibited the expression of inflammatory factors in the brain tissues of MCAO rats. The neuroprotective effects of HXP on cerebral ischemic injuries were compromised by MCPIP1 knockdown. Immunofluorescence results indicated that the expression of microglia marker Iba1 and M2 phenotypic marker CD206 was upregulated in MCAO rats and OGD/R-treated microglia. Administration of HXP significantly reduced Iba1 expression and facilitated CD206 expression, an effect that was counteracted by sh-MCPIP1 transfection. Western blotting revealed that HXP treatment augmented the expression of MCPIP1, microglial M2 marker proteins (CD206 and Arg1), and PPARγ, while reducing the expression of microglial M1 marker proteins (CD16 and iNOS) in MCAO rats and OGD/R-induced microglia. MCPIP1 knockdown suppressed HXP-mediated upregulation of MCPIP1, CD206, Arg1, and PPARγ, as well as the downregulation of CD16 and iNOS. Our findings suggest that HXP primarily ameliorates ischemic stroke through the upregulation of MCPIP1, which in turn induces microglial M2 polarization. |
| first_indexed | 2024-03-13T04:56:52Z |
| format | Article |
| id | doaj.art-8dbed743abd943389109a59b427de7fb |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-03-13T04:56:52Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-8dbed743abd943389109a59b427de7fb2023-06-18T05:00:41ZengElsevierBiomedicine & Pharmacotherapy0753-33222023-08-01164114914Huoluo Xiaoling Pellet promotes microglia M2 polarization through increasing MCPIP1 expression for ischemia stroke alleviationWei Shen0Xiaoguang Wang1Meiqi Tang2Lan Yao3Chenyu Wan4Jianli Niu5Pappachan E. Kolattukudy6Zhuqing Jin7The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), No.54 Youdian Road, Hangzhou 310000, ChinaSchool of Life Science, Xiamen University, No.4221-120, Xiangan North Road, Xiamen 361100, ChinaDepartment of Chemistry, Zhejiang University, No.38 Zheda Road, Hangzhou 310027, ChinaDepartment of Chemistry, Zhejiang University, No.38 Zheda Road, Hangzhou 310027, ChinaThe Affiliated Hospital of Hangzhou Normal University, No.126 Wenzhou Road, Hangzhou 310015, ChinaBurnett School of Biomedical Sciences, University of Central Florida College of Medicine, 4000 Central Florida Blvd, Orlando, United StatesBurnett School of Biomedical Sciences, University of Central Florida College of Medicine, 4000 Central Florida Blvd, Orlando, United StatesSchool of Basic Medical Sciences, Zhejiang Chinese Medical University, No.548 Binwen Road, Hangzhou 310053, China; Corresponding author.Huoluo Xiaoling Pellet (HXP), a Chinese patent medicine, is commonly administered for the treatment of treat ischemic strokes. MCPIP1, an inducible suppressor of the inflammatory response, is a regulator of microglial M2 polarization. This study aimed to explore whether HXP can promote microglial M2 polarization by upregulating MCPIP1 expression, consequently mitigating cerebral ischemic injury. Our study involved 85 Sprague-Dawley rats (weighing 250–280 g). We established middle cerebral artery occlusion (MCAO) and oxygen-glucose deprivation-reoxygenation (OGD/R) models with MCPIP1 knockdown to assess the effects of HXP on ischemic strokes. Our findings show that HXP reduced brain water content, improved neurological function, and inhibited the expression of inflammatory factors in the brain tissues of MCAO rats. The neuroprotective effects of HXP on cerebral ischemic injuries were compromised by MCPIP1 knockdown. Immunofluorescence results indicated that the expression of microglia marker Iba1 and M2 phenotypic marker CD206 was upregulated in MCAO rats and OGD/R-treated microglia. Administration of HXP significantly reduced Iba1 expression and facilitated CD206 expression, an effect that was counteracted by sh-MCPIP1 transfection. Western blotting revealed that HXP treatment augmented the expression of MCPIP1, microglial M2 marker proteins (CD206 and Arg1), and PPARγ, while reducing the expression of microglial M1 marker proteins (CD16 and iNOS) in MCAO rats and OGD/R-induced microglia. MCPIP1 knockdown suppressed HXP-mediated upregulation of MCPIP1, CD206, Arg1, and PPARγ, as well as the downregulation of CD16 and iNOS. Our findings suggest that HXP primarily ameliorates ischemic stroke through the upregulation of MCPIP1, which in turn induces microglial M2 polarization.http://www.sciencedirect.com/science/article/pii/S0753332223007047Huoluo Xiaoling Pellet (HXP)Ischemia strokeMCPIP1MicrogliaM2 polarization |
| spellingShingle | Wei Shen Xiaoguang Wang Meiqi Tang Lan Yao Chenyu Wan Jianli Niu Pappachan E. Kolattukudy Zhuqing Jin Huoluo Xiaoling Pellet promotes microglia M2 polarization through increasing MCPIP1 expression for ischemia stroke alleviation Biomedicine & Pharmacotherapy Huoluo Xiaoling Pellet (HXP) Ischemia stroke MCPIP1 Microglia M2 polarization |
| title | Huoluo Xiaoling Pellet promotes microglia M2 polarization through increasing MCPIP1 expression for ischemia stroke alleviation |
| title_full | Huoluo Xiaoling Pellet promotes microglia M2 polarization through increasing MCPIP1 expression for ischemia stroke alleviation |
| title_fullStr | Huoluo Xiaoling Pellet promotes microglia M2 polarization through increasing MCPIP1 expression for ischemia stroke alleviation |
| title_full_unstemmed | Huoluo Xiaoling Pellet promotes microglia M2 polarization through increasing MCPIP1 expression for ischemia stroke alleviation |
| title_short | Huoluo Xiaoling Pellet promotes microglia M2 polarization through increasing MCPIP1 expression for ischemia stroke alleviation |
| title_sort | huoluo xiaoling pellet promotes microglia m2 polarization through increasing mcpip1 expression for ischemia stroke alleviation |
| topic | Huoluo Xiaoling Pellet (HXP) Ischemia stroke MCPIP1 Microglia M2 polarization |
| url | http://www.sciencedirect.com/science/article/pii/S0753332223007047 |
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