A comparative review on TLQP-21 receptors: rodent versus human
Growing functional information regarding bioactive TLQP-21 has led to the ubiquitous demand for identification of receptors associated to the peptide, which resulted in the first wave of murine TLQP-21 receptors, gC1qR and C3AR1. gC1qR was identified as receptor of TLQP-21 using chemical crosslinkin...
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| Format: | Article |
| Language: | English |
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Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology
2020-04-01
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| Series: | Journal of Advanced Biotechnology and Experimental Therapeutics |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=68142 |
| _version_ | 1818624278073966592 |
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| author | Md Shamim Akhter |
| author_facet | Md Shamim Akhter |
| author_sort | Md Shamim Akhter |
| collection | DOAJ |
| description | Growing functional information regarding bioactive TLQP-21 has led to the ubiquitous demand for identification of receptors associated to the peptide, which resulted in the first wave of murine TLQP-21 receptors, gC1qR and C3AR1. gC1qR was identified as receptor of TLQP-21 using chemical crosslinking and monomeric avidin column purification following by MS analysis. TLQP-21 responsive CHO-K1 cells were used to search for its receptor, C3AR1. Putative GPCRs, which may partake in regulating intracellular biological functions induced by TLQP-21, were indexed after the CHO-K1 cellular transcriptome was sequenced using unbiased Genome Wide Sequencing. TLQP-21 binding in the cells were found to be reduced by the gene knockdown with the siRNAs targeting C3AR1. C3AR1 antagonist, SB290157 was shown to prohibit TLQP-21 activity in CHOK1 cells. But this finding was not demonstrable in human cell line. The differences of human TLQP-21 sequence with that of murine TLQP-21 explains the poor binding of the human orthologue with its corresponding receptor. This may suggest a different set of receptors when considering human and rodent variants of TLQP-21. The identification of HSPA8 as receptor was performed using affinity based chromatography and mass spectrometry from human SHSY-5Y cells. Molecular studies in silico revealed that the peptide binding pocket in HSPA8 is an appropriate fit for TLQP-21 docking. Cross-linking and FACS methods presented the TLQP-21 binding to cells from the SHSY-5Y line. The establishment of HSPA8 as a putative receptor for human TLQP-21 can be exploited to explore new horizon in diagnosis and therapies for VGF related human diseases. [ J Adv Biotechnol Exp Ther 2020; 3(1.000): 09-19] |
| first_indexed | 2024-12-16T18:54:25Z |
| format | Article |
| id | doaj.art-8dc40fd98b2d4516ac6c000c90cc620d |
| institution | Directory Open Access Journal |
| issn | 2616-4760 |
| language | English |
| last_indexed | 2024-12-16T18:54:25Z |
| publishDate | 2020-04-01 |
| publisher | Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology |
| record_format | Article |
| series | Journal of Advanced Biotechnology and Experimental Therapeutics |
| spelling | doaj.art-8dc40fd98b2d4516ac6c000c90cc620d2022-12-21T22:20:35ZengBangladesh Society for Microbiology, Immunology, and Advanced BiotechnologyJournal of Advanced Biotechnology and Experimental Therapeutics2616-47602020-04-0131091910.5455/jabet.2020.d10268142A comparative review on TLQP-21 receptors: rodent versus humanMd Shamim Akhter0Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna, BangladeshGrowing functional information regarding bioactive TLQP-21 has led to the ubiquitous demand for identification of receptors associated to the peptide, which resulted in the first wave of murine TLQP-21 receptors, gC1qR and C3AR1. gC1qR was identified as receptor of TLQP-21 using chemical crosslinking and monomeric avidin column purification following by MS analysis. TLQP-21 responsive CHO-K1 cells were used to search for its receptor, C3AR1. Putative GPCRs, which may partake in regulating intracellular biological functions induced by TLQP-21, were indexed after the CHO-K1 cellular transcriptome was sequenced using unbiased Genome Wide Sequencing. TLQP-21 binding in the cells were found to be reduced by the gene knockdown with the siRNAs targeting C3AR1. C3AR1 antagonist, SB290157 was shown to prohibit TLQP-21 activity in CHOK1 cells. But this finding was not demonstrable in human cell line. The differences of human TLQP-21 sequence with that of murine TLQP-21 explains the poor binding of the human orthologue with its corresponding receptor. This may suggest a different set of receptors when considering human and rodent variants of TLQP-21. The identification of HSPA8 as receptor was performed using affinity based chromatography and mass spectrometry from human SHSY-5Y cells. Molecular studies in silico revealed that the peptide binding pocket in HSPA8 is an appropriate fit for TLQP-21 docking. Cross-linking and FACS methods presented the TLQP-21 binding to cells from the SHSY-5Y line. The establishment of HSPA8 as a putative receptor for human TLQP-21 can be exploited to explore new horizon in diagnosis and therapies for VGF related human diseases. [ J Adv Biotechnol Exp Ther 2020; 3(1.000): 09-19]http://www.ejmanager.com/fulltextpdf.php?mno=68142vgftlqp-21hspa8shsy-5yreceptor |
| spellingShingle | Md Shamim Akhter A comparative review on TLQP-21 receptors: rodent versus human Journal of Advanced Biotechnology and Experimental Therapeutics vgf tlqp-21 hspa8 shsy-5y receptor |
| title | A comparative review on TLQP-21 receptors: rodent versus human |
| title_full | A comparative review on TLQP-21 receptors: rodent versus human |
| title_fullStr | A comparative review on TLQP-21 receptors: rodent versus human |
| title_full_unstemmed | A comparative review on TLQP-21 receptors: rodent versus human |
| title_short | A comparative review on TLQP-21 receptors: rodent versus human |
| title_sort | comparative review on tlqp 21 receptors rodent versus human |
| topic | vgf tlqp-21 hspa8 shsy-5y receptor |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=68142 |
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