Analyzing the gonadal transcriptome of the frog Hoplobatrachus rugulosus to identify genes involved in sex development
Abstract Background The tiger frog (Hoplobatrachus rugulosus) is listed as a national Class II protected species in China. In the context of global warming, the sex ratio of amphibians will be affected, and the development of the population will be limited. Therefore, considering the potential for a...
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| Format: | Article |
| Language: | English |
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BMC
2021-07-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-021-07879-6 |
| _version_ | 1819139871269191680 |
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| author | Yun Tang Jing-Yi Chen Guo-Hua Ding Zhi-Hua Lin |
| author_facet | Yun Tang Jing-Yi Chen Guo-Hua Ding Zhi-Hua Lin |
| author_sort | Yun Tang |
| collection | DOAJ |
| description | Abstract Background The tiger frog (Hoplobatrachus rugulosus) is listed as a national Class II protected species in China. In the context of global warming, the sex ratio of amphibians will be affected, and the development of the population will be limited. Therefore, considering the potential for a decrease in the number of amphibians, studying sex evolution and molecular regulation of gonadal development in H. rugulosus, phenomenon that are currently unclear, is of great significance. Results Here, H. rugulosus was used to explore the mechanisms regulating gonadal development in amphibians. Illumina HiSeq 3000 was used to sequence the gonadal transcriptome of male and female H. rugulosus at two growth stages to identify genes related to gonadal development and analyze expression differences in the gonads. This analysis indicated that cyp17α, hsd3β, hsd11β1, cyp19α, and hsd17β12 perform vital functions in sex development in amphibians. Specifically, the expression of cyp3α, cyp17α, hsd3β, hsd11β1, sox2, sox9, sox30, soat, cyp19α, hsd17β12, and hspα1s was correlated with gonadal development and differentiation in H. rugulosus, as determined using the quantitative reverse transcriptase-polymerase chain reaction. Conclusion Significant differences were found in the gonadal gene expression levels in H. rugulosus of both sexes, and we identified a steroid hormone synthesis pathway in this species and analyzed related gene expression, but the changes during sex differentiation were still unclear. To our knowledge, this report presents the first analysis of the H. rugulosus gonadal transcriptome and lays the foundation for future research. |
| first_indexed | 2024-12-22T11:29:33Z |
| format | Article |
| id | doaj.art-8dc7c6a8f0c643cca0ac46f197297204 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-22T11:29:33Z |
| publishDate | 2021-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-8dc7c6a8f0c643cca0ac46f1972972042022-12-21T18:27:39ZengBMCBMC Genomics1471-21642021-07-0122111310.1186/s12864-021-07879-6Analyzing the gonadal transcriptome of the frog Hoplobatrachus rugulosus to identify genes involved in sex developmentYun Tang0Jing-Yi Chen1Guo-Hua Ding2Zhi-Hua Lin3Laboratory of Amphibian Diversity Investigation, College of Ecology, Lishui UniversityLaboratory of Amphibian Diversity Investigation, College of Ecology, Lishui UniversityLaboratory of Amphibian Diversity Investigation, College of Ecology, Lishui UniversityLaboratory of Amphibian Diversity Investigation, College of Ecology, Lishui UniversityAbstract Background The tiger frog (Hoplobatrachus rugulosus) is listed as a national Class II protected species in China. In the context of global warming, the sex ratio of amphibians will be affected, and the development of the population will be limited. Therefore, considering the potential for a decrease in the number of amphibians, studying sex evolution and molecular regulation of gonadal development in H. rugulosus, phenomenon that are currently unclear, is of great significance. Results Here, H. rugulosus was used to explore the mechanisms regulating gonadal development in amphibians. Illumina HiSeq 3000 was used to sequence the gonadal transcriptome of male and female H. rugulosus at two growth stages to identify genes related to gonadal development and analyze expression differences in the gonads. This analysis indicated that cyp17α, hsd3β, hsd11β1, cyp19α, and hsd17β12 perform vital functions in sex development in amphibians. Specifically, the expression of cyp3α, cyp17α, hsd3β, hsd11β1, sox2, sox9, sox30, soat, cyp19α, hsd17β12, and hspα1s was correlated with gonadal development and differentiation in H. rugulosus, as determined using the quantitative reverse transcriptase-polymerase chain reaction. Conclusion Significant differences were found in the gonadal gene expression levels in H. rugulosus of both sexes, and we identified a steroid hormone synthesis pathway in this species and analyzed related gene expression, but the changes during sex differentiation were still unclear. To our knowledge, this report presents the first analysis of the H. rugulosus gonadal transcriptome and lays the foundation for future research.https://doi.org/10.1186/s12864-021-07879-6AssemblyGeneGonadal developmentHoplobatrachus rugulosusMolecular pathwayTranscriptome |
| spellingShingle | Yun Tang Jing-Yi Chen Guo-Hua Ding Zhi-Hua Lin Analyzing the gonadal transcriptome of the frog Hoplobatrachus rugulosus to identify genes involved in sex development BMC Genomics Assembly Gene Gonadal development Hoplobatrachus rugulosus Molecular pathway Transcriptome |
| title | Analyzing the gonadal transcriptome of the frog Hoplobatrachus rugulosus to identify genes involved in sex development |
| title_full | Analyzing the gonadal transcriptome of the frog Hoplobatrachus rugulosus to identify genes involved in sex development |
| title_fullStr | Analyzing the gonadal transcriptome of the frog Hoplobatrachus rugulosus to identify genes involved in sex development |
| title_full_unstemmed | Analyzing the gonadal transcriptome of the frog Hoplobatrachus rugulosus to identify genes involved in sex development |
| title_short | Analyzing the gonadal transcriptome of the frog Hoplobatrachus rugulosus to identify genes involved in sex development |
| title_sort | analyzing the gonadal transcriptome of the frog hoplobatrachus rugulosus to identify genes involved in sex development |
| topic | Assembly Gene Gonadal development Hoplobatrachus rugulosus Molecular pathway Transcriptome |
| url | https://doi.org/10.1186/s12864-021-07879-6 |
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