Antioxidant activities of novel galloyl phytosterols evaluated by human erythrocytes with the aid of confocal microscopy imaging
Notable protective effect of the novel galloyl phytosterol antioxidant on human erythrocytes was disclosed in this study. 2,2′-Azo-bis (2-amidinopropane) hydrochloride (AAPH) induced haemolysis of erythrocytes, and depletion of cellular glutathione were effectively inhibited by the galloyl phytoster...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-04-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464616000293 |
| _version_ | 1819292720037888000 |
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| author | Songbai Liu Huiying Hu Cheng Li |
| author_facet | Songbai Liu Huiying Hu Cheng Li |
| author_sort | Songbai Liu |
| collection | DOAJ |
| description | Notable protective effect of the novel galloyl phytosterol antioxidant on human erythrocytes was disclosed in this study. 2,2′-Azo-bis (2-amidinopropane) hydrochloride (AAPH) induced haemolysis of erythrocytes, and depletion of cellular glutathione were effectively inhibited by the galloyl phytosterol antioxidant. It was revealed that the level of glutathione was a more sensitive indicator than haemolysis for cellular oxidative stress. The morphology of erythrocytes obtained through laser scanning confocal microscopy (LSCM) imaging provided structural details for haemolysis and glutathione investigation and was more sensitive than the level of glutathione. The liposome model exhibited that the novel antioxidant preferred to anchor in the membrane and had better performance for membrane protection than gallic acid. The strategy of integrated use of biochemical and visual methods developed in this study is valuable for investigation on mechanistic behaviours of antioxidants. |
| first_indexed | 2024-12-24T03:59:01Z |
| format | Article |
| id | doaj.art-8dd16112edf940d59b3b1d7b78be7bb2 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-24T03:59:01Z |
| publishDate | 2016-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-8dd16112edf940d59b3b1d7b78be7bb22022-12-21T17:16:21ZengElsevierJournal of Functional Foods1756-46462016-04-0122224231Antioxidant activities of novel galloyl phytosterols evaluated by human erythrocytes with the aid of confocal microscopy imagingSongbai Liu0Huiying Hu1Cheng Li2Corresponding author. Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China. Tel.: +86 571 88982186; fax: +86 571 88982186.; Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaNotable protective effect of the novel galloyl phytosterol antioxidant on human erythrocytes was disclosed in this study. 2,2′-Azo-bis (2-amidinopropane) hydrochloride (AAPH) induced haemolysis of erythrocytes, and depletion of cellular glutathione were effectively inhibited by the galloyl phytosterol antioxidant. It was revealed that the level of glutathione was a more sensitive indicator than haemolysis for cellular oxidative stress. The morphology of erythrocytes obtained through laser scanning confocal microscopy (LSCM) imaging provided structural details for haemolysis and glutathione investigation and was more sensitive than the level of glutathione. The liposome model exhibited that the novel antioxidant preferred to anchor in the membrane and had better performance for membrane protection than gallic acid. The strategy of integrated use of biochemical and visual methods developed in this study is valuable for investigation on mechanistic behaviours of antioxidants.http://www.sciencedirect.com/science/article/pii/S1756464616000293PhytosterolGallic acidGalloyl phytosterolAntioxidant activityErythrocyte haemolysisLaser scanning confocal microscopy |
| spellingShingle | Songbai Liu Huiying Hu Cheng Li Antioxidant activities of novel galloyl phytosterols evaluated by human erythrocytes with the aid of confocal microscopy imaging Journal of Functional Foods Phytosterol Gallic acid Galloyl phytosterol Antioxidant activity Erythrocyte haemolysis Laser scanning confocal microscopy |
| title | Antioxidant activities of novel galloyl phytosterols evaluated by human erythrocytes with the aid of confocal microscopy imaging |
| title_full | Antioxidant activities of novel galloyl phytosterols evaluated by human erythrocytes with the aid of confocal microscopy imaging |
| title_fullStr | Antioxidant activities of novel galloyl phytosterols evaluated by human erythrocytes with the aid of confocal microscopy imaging |
| title_full_unstemmed | Antioxidant activities of novel galloyl phytosterols evaluated by human erythrocytes with the aid of confocal microscopy imaging |
| title_short | Antioxidant activities of novel galloyl phytosterols evaluated by human erythrocytes with the aid of confocal microscopy imaging |
| title_sort | antioxidant activities of novel galloyl phytosterols evaluated by human erythrocytes with the aid of confocal microscopy imaging |
| topic | Phytosterol Gallic acid Galloyl phytosterol Antioxidant activity Erythrocyte haemolysis Laser scanning confocal microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S1756464616000293 |
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