Comparison of electroretinographic responses between two different age groups of adult Dark Agouti rats
<b>AIM:</b> To describe and compare the differences in electroretinographic responses between two different age groups of adult Dark Agouti (DA) rats and to better understand the effect of age on retinal histology and function.<b>METHODS:</b> The electroretinographic response...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2015-10-01
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| Series: | International Journal of Ophthalmology |
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| Online Access: | http://www.ijo.cn/en_publish/2015/5/20150508.pdf |
| _version_ | 1819243513423855616 |
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| author | Lin Fu Amy Cheuk Yin Lo Jimmy Shiu Ming Lai Kendrick Co Shih |
| author_facet | Lin Fu Amy Cheuk Yin Lo Jimmy Shiu Ming Lai Kendrick Co Shih |
| author_sort | Lin Fu |
| collection | DOAJ |
| description | <b>AIM:</b> To describe and compare the differences in electroretinographic responses between two different age groups of adult Dark Agouti (DA) rats and to better understand the effect of age on retinal histology and function.<b>METHODS:</b> The electroretinographic responses of two different age groups of adult DA rats were compared. Animals were divided into younger adult DA rats 10-12wk (<i>n</i>=8) and older adult DA rats 17-19wk (<i>n</i>=8). Full field electroretinography (ERG) was recorded simultaneously from both eyes after dark adaption and light adaption and parameters including the positive scotopic threshold response (pSTR), negative scotopic threshold response (nSTR), scotopic a-wave, b-wave, photopic a-wave, b-wave and photopic negative response (PhNR) were compared between groups.<b>RESULTS:</b> The older adult rats displayed lower stimulation thresholds of the STRs (pSTR and nSTR) and higher amplitudes of pSTR, scotopic a-wave and b-wave, photopic b-wave and PhNR amplitudes, with shorter implicit times. Photopic a-wave amplitudes were however higher in the younger adult rats.<b>CONCLUSION</b>:In summary, for the rod system, photoreceptor, bipolar cell and RGC activity was enhanced in the older adult rats. For the cone system, RGC and bipolar cell activity was enhanced, while photoreceptor activity was depressed in the older adult rats. Such age-related selective modification of retinal cell function needs to be considered when conducting ophthalmic research in adult rats. |
| first_indexed | 2024-12-23T14:56:54Z |
| format | Article |
| id | doaj.art-8dd206944f5f461dba5c62c33bc5b170 |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-23T14:56:54Z |
| publishDate | 2015-10-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-8dd206944f5f461dba5c62c33bc5b1702022-12-21T17:42:45ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982015-10-018589890310.3980/j.issn.2222-3959.2015.05.08Comparison of electroretinographic responses between two different age groups of adult Dark Agouti ratsLin Fu0Amy Cheuk Yin Lo1Jimmy Shiu Ming Lai2Kendrick Co Shih3Department of Ophthalmology, LKS Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong, ChinaDepartment of Ophthalmology, LKS Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong, ChinaDepartment of Ophthalmology, LKS Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong, ChinaDepartment of Ophthalmology, LKS Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong, China<b>AIM:</b> To describe and compare the differences in electroretinographic responses between two different age groups of adult Dark Agouti (DA) rats and to better understand the effect of age on retinal histology and function.<b>METHODS:</b> The electroretinographic responses of two different age groups of adult DA rats were compared. Animals were divided into younger adult DA rats 10-12wk (<i>n</i>=8) and older adult DA rats 17-19wk (<i>n</i>=8). Full field electroretinography (ERG) was recorded simultaneously from both eyes after dark adaption and light adaption and parameters including the positive scotopic threshold response (pSTR), negative scotopic threshold response (nSTR), scotopic a-wave, b-wave, photopic a-wave, b-wave and photopic negative response (PhNR) were compared between groups.<b>RESULTS:</b> The older adult rats displayed lower stimulation thresholds of the STRs (pSTR and nSTR) and higher amplitudes of pSTR, scotopic a-wave and b-wave, photopic b-wave and PhNR amplitudes, with shorter implicit times. Photopic a-wave amplitudes were however higher in the younger adult rats.<b>CONCLUSION</b>:In summary, for the rod system, photoreceptor, bipolar cell and RGC activity was enhanced in the older adult rats. For the cone system, RGC and bipolar cell activity was enhanced, while photoreceptor activity was depressed in the older adult rats. Such age-related selective modification of retinal cell function needs to be considered when conducting ophthalmic research in adult rats.http://www.ijo.cn/en_publish/2015/5/20150508.pdfelectroretinographic responsesDark Agouti ratsage |
| spellingShingle | Lin Fu Amy Cheuk Yin Lo Jimmy Shiu Ming Lai Kendrick Co Shih Comparison of electroretinographic responses between two different age groups of adult Dark Agouti rats International Journal of Ophthalmology electroretinographic responses Dark Agouti rats age |
| title | Comparison of electroretinographic responses between two different age groups of adult Dark Agouti rats |
| title_full | Comparison of electroretinographic responses between two different age groups of adult Dark Agouti rats |
| title_fullStr | Comparison of electroretinographic responses between two different age groups of adult Dark Agouti rats |
| title_full_unstemmed | Comparison of electroretinographic responses between two different age groups of adult Dark Agouti rats |
| title_short | Comparison of electroretinographic responses between two different age groups of adult Dark Agouti rats |
| title_sort | comparison of electroretinographic responses between two different age groups of adult dark agouti rats |
| topic | electroretinographic responses Dark Agouti rats age |
| url | http://www.ijo.cn/en_publish/2015/5/20150508.pdf |
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