Sensitive Detection of C-Reactive Protein by One-Step Method Based on a Waveguide-Mode Sensor
One-step biosensing methods enable the quick and simplified detection of biological substances. In this study, we developed a sensitive one-step method on the basis of a waveguide-mode sensor, which is an optical sensor utilizing waveguide-mode resonance and evanescent light. Streptavidin-conjugated...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-06-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/20/11/3195 |
| _version_ | 1797566100313997312 |
|---|---|
| author | Hiroki Ashiba Chiaki Oyamada Kazuya Hosokawa Koji Ueno Makoto Fujimaki |
| author_facet | Hiroki Ashiba Chiaki Oyamada Kazuya Hosokawa Koji Ueno Makoto Fujimaki |
| author_sort | Hiroki Ashiba |
| collection | DOAJ |
| description | One-step biosensing methods enable the quick and simplified detection of biological substances. In this study, we developed a sensitive one-step method on the basis of a waveguide-mode sensor, which is an optical sensor utilizing waveguide-mode resonance and evanescent light. Streptavidin-conjugated and gold-nanoparticle-conjugated antibodies were reacted with a target substance and applied onto a biotinylated sensing plate. The target substance was detected by observing changes in sensor signals caused by binding the immunocomplex to the sensing surface. Performance of the developed one-step method was examined using a C-reactive protein (CRP) as a target substance. A sensor signal corresponding to the concentration of CRP was obtained. The minimal detectable CRP concentration of the developed method was 10 pM. The developed method greatly simplifies quantitative protein detection without reducing sensitivity. |
| first_indexed | 2024-03-10T19:21:55Z |
| format | Article |
| id | doaj.art-8dd217ef80714e6e92c2008ab2273f0c |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T19:21:55Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-8dd217ef80714e6e92c2008ab2273f0c2023-11-20T02:53:45ZengMDPI AGSensors1424-82202020-06-012011319510.3390/s20113195Sensitive Detection of C-Reactive Protein by One-Step Method Based on a Waveguide-Mode SensorHiroki Ashiba0Chiaki Oyamada1Kazuya Hosokawa2Koji Ueno3Makoto Fujimaki4Sensing System Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, JapanResearch Institute, Fujimori Kogyo Co., Ltd., 1-10-1 Sachiura, Kanazawa-ku, Yokohama, Kanagawa 236-0003, JapanResearch Institute, Fujimori Kogyo Co., Ltd., 1-10-1 Sachiura, Kanazawa-ku, Yokohama, Kanagawa 236-0003, JapanC&I Co., Ltd., 2004-1 Tamatori, Tsukuba, Ibaraki 300-3255, JapanSensing System Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, JapanOne-step biosensing methods enable the quick and simplified detection of biological substances. In this study, we developed a sensitive one-step method on the basis of a waveguide-mode sensor, which is an optical sensor utilizing waveguide-mode resonance and evanescent light. Streptavidin-conjugated and gold-nanoparticle-conjugated antibodies were reacted with a target substance and applied onto a biotinylated sensing plate. The target substance was detected by observing changes in sensor signals caused by binding the immunocomplex to the sensing surface. Performance of the developed one-step method was examined using a C-reactive protein (CRP) as a target substance. A sensor signal corresponding to the concentration of CRP was obtained. The minimal detectable CRP concentration of the developed method was 10 pM. The developed method greatly simplifies quantitative protein detection without reducing sensitivity.https://www.mdpi.com/1424-8220/20/11/3195immunoassayone-step methodoptical sensorevanescent lightwaveguide-mode resonancegold nanoparticle |
| spellingShingle | Hiroki Ashiba Chiaki Oyamada Kazuya Hosokawa Koji Ueno Makoto Fujimaki Sensitive Detection of C-Reactive Protein by One-Step Method Based on a Waveguide-Mode Sensor Sensors immunoassay one-step method optical sensor evanescent light waveguide-mode resonance gold nanoparticle |
| title | Sensitive Detection of C-Reactive Protein by One-Step Method Based on a Waveguide-Mode Sensor |
| title_full | Sensitive Detection of C-Reactive Protein by One-Step Method Based on a Waveguide-Mode Sensor |
| title_fullStr | Sensitive Detection of C-Reactive Protein by One-Step Method Based on a Waveguide-Mode Sensor |
| title_full_unstemmed | Sensitive Detection of C-Reactive Protein by One-Step Method Based on a Waveguide-Mode Sensor |
| title_short | Sensitive Detection of C-Reactive Protein by One-Step Method Based on a Waveguide-Mode Sensor |
| title_sort | sensitive detection of c reactive protein by one step method based on a waveguide mode sensor |
| topic | immunoassay one-step method optical sensor evanescent light waveguide-mode resonance gold nanoparticle |
| url | https://www.mdpi.com/1424-8220/20/11/3195 |
| work_keys_str_mv | AT hirokiashiba sensitivedetectionofcreactiveproteinbyonestepmethodbasedonawaveguidemodesensor AT chiakioyamada sensitivedetectionofcreactiveproteinbyonestepmethodbasedonawaveguidemodesensor AT kazuyahosokawa sensitivedetectionofcreactiveproteinbyonestepmethodbasedonawaveguidemodesensor AT kojiueno sensitivedetectionofcreactiveproteinbyonestepmethodbasedonawaveguidemodesensor AT makotofujimaki sensitivedetectionofcreactiveproteinbyonestepmethodbasedonawaveguidemodesensor |