Anti-human lung adenocarcinoma, cytotoxicity, and antioxidant potentials of copper nanoparticles green-synthesized by Calendula officinalis
Calendula officinalis is known as a popular plant with various uses as pharmaceutical agent or food additive around the world. In this study, cooper nanoparticles were green synthesized using the aqueous extract of Calendula officinalis as the stabilizing, reducing, and capping agents. The formation...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2022-12-01
|
| Series: | Journal of Experimental Nanoscience |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/17458080.2022.2066082 |
| _version_ | 1811332278687629312 |
|---|---|
| author | Jianfa Gu Ali Aidy Samaneh Goorani |
| author_facet | Jianfa Gu Ali Aidy Samaneh Goorani |
| author_sort | Jianfa Gu |
| collection | DOAJ |
| description | Calendula officinalis is known as a popular plant with various uses as pharmaceutical agent or food additive around the world. In this study, cooper nanoparticles were green synthesized using the aqueous extract of Calendula officinalis as the stabilizing, reducing, and capping agents. The formation of CuNPs@C. officinalis was screened using different chemical technique such as FT-IR spectroscopy, XRD, SEM, and Energy EDS. The antioxidant and anti-cancer activity of CuNPs@C. officinalis was studied using common assay including free radical scavenging and MTT assays. The results confirm the green synthesized of CuNPs@C. officinalis with aspherical morphology in the range size of 19.64 to 39.15 nm. In the antioxidant test, the IC50 of CuNPs@C. officinalis and BHT against DPPH free radicals were 193 and 154 µg/mL, respectively. In the cellular and molecular part of the recent study, the treated cells with CuNPs@C. officinalis were assessed by MTT assay for 24, 48, and 72 h about the cytotoxicity and anti-human lung adenocarcinoma properties on normal (HUVEC) and lung adenocarcinoma cell lines, i.e. lung moderately differentiated adenocarcinoma (LC-2/ad), lung poorly differentiated adenocarcinoma (PC-14), and lung well-differentiated bronchogenic adenocarcinoma (HLC-1). The IC50s of CuNPs@C. officinalis were 297, 328, and 514 µg/mL against PC-14, LC-2/ad, and HLC-1 cell lines, respectively. The viability of malignant lung cell line reduced dose-dependently in the presence of CuNPs@C. officinalis. It seems that the anti-human lung adenocarcinoma effect of recent nanoparticles is due to their antioxidant effects. |
| first_indexed | 2024-04-13T16:34:16Z |
| format | Article |
| id | doaj.art-8dd2785141b8468fad9758e34aab8246 |
| institution | Directory Open Access Journal |
| issn | 1745-8080 1745-8099 |
| language | English |
| last_indexed | 2024-04-13T16:34:16Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Experimental Nanoscience |
| spelling | doaj.art-8dd2785141b8468fad9758e34aab82462022-12-22T02:39:29ZengTaylor & Francis GroupJournal of Experimental Nanoscience1745-80801745-80992022-12-0117128529610.1080/17458080.2022.2066082Anti-human lung adenocarcinoma, cytotoxicity, and antioxidant potentials of copper nanoparticles green-synthesized by Calendula officinalisJianfa Gu0Ali Aidy1Samaneh Goorani2Department of Oncology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou City, ChinaBiotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, IranDepartment of Toxicology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranCalendula officinalis is known as a popular plant with various uses as pharmaceutical agent or food additive around the world. In this study, cooper nanoparticles were green synthesized using the aqueous extract of Calendula officinalis as the stabilizing, reducing, and capping agents. The formation of CuNPs@C. officinalis was screened using different chemical technique such as FT-IR spectroscopy, XRD, SEM, and Energy EDS. The antioxidant and anti-cancer activity of CuNPs@C. officinalis was studied using common assay including free radical scavenging and MTT assays. The results confirm the green synthesized of CuNPs@C. officinalis with aspherical morphology in the range size of 19.64 to 39.15 nm. In the antioxidant test, the IC50 of CuNPs@C. officinalis and BHT against DPPH free radicals were 193 and 154 µg/mL, respectively. In the cellular and molecular part of the recent study, the treated cells with CuNPs@C. officinalis were assessed by MTT assay for 24, 48, and 72 h about the cytotoxicity and anti-human lung adenocarcinoma properties on normal (HUVEC) and lung adenocarcinoma cell lines, i.e. lung moderately differentiated adenocarcinoma (LC-2/ad), lung poorly differentiated adenocarcinoma (PC-14), and lung well-differentiated bronchogenic adenocarcinoma (HLC-1). The IC50s of CuNPs@C. officinalis were 297, 328, and 514 µg/mL against PC-14, LC-2/ad, and HLC-1 cell lines, respectively. The viability of malignant lung cell line reduced dose-dependently in the presence of CuNPs@C. officinalis. It seems that the anti-human lung adenocarcinoma effect of recent nanoparticles is due to their antioxidant effects.https://www.tandfonline.com/doi/10.1080/17458080.2022.2066082Cu nanoparticleschemical characterizationhuman lung adenocarcinomaantioxidant activity |
| spellingShingle | Jianfa Gu Ali Aidy Samaneh Goorani Anti-human lung adenocarcinoma, cytotoxicity, and antioxidant potentials of copper nanoparticles green-synthesized by Calendula officinalis Journal of Experimental Nanoscience Cu nanoparticles chemical characterization human lung adenocarcinoma antioxidant activity |
| title | Anti-human lung adenocarcinoma, cytotoxicity, and antioxidant potentials of copper nanoparticles green-synthesized by Calendula officinalis |
| title_full | Anti-human lung adenocarcinoma, cytotoxicity, and antioxidant potentials of copper nanoparticles green-synthesized by Calendula officinalis |
| title_fullStr | Anti-human lung adenocarcinoma, cytotoxicity, and antioxidant potentials of copper nanoparticles green-synthesized by Calendula officinalis |
| title_full_unstemmed | Anti-human lung adenocarcinoma, cytotoxicity, and antioxidant potentials of copper nanoparticles green-synthesized by Calendula officinalis |
| title_short | Anti-human lung adenocarcinoma, cytotoxicity, and antioxidant potentials of copper nanoparticles green-synthesized by Calendula officinalis |
| title_sort | anti human lung adenocarcinoma cytotoxicity and antioxidant potentials of copper nanoparticles green synthesized by calendula officinalis |
| topic | Cu nanoparticles chemical characterization human lung adenocarcinoma antioxidant activity |
| url | https://www.tandfonline.com/doi/10.1080/17458080.2022.2066082 |
| work_keys_str_mv | AT jianfagu antihumanlungadenocarcinomacytotoxicityandantioxidantpotentialsofcoppernanoparticlesgreensynthesizedbycalendulaofficinalis AT aliaidy antihumanlungadenocarcinomacytotoxicityandantioxidantpotentialsofcoppernanoparticlesgreensynthesizedbycalendulaofficinalis AT samanehgoorani antihumanlungadenocarcinomacytotoxicityandantioxidantpotentialsofcoppernanoparticlesgreensynthesizedbycalendulaofficinalis |