Altered Gut Microbiota Related to Inflammatory Responses in Patients With Huntington’s Disease
Emerging evidence indicates that gut dysbiosis may play a regulatory role in the onset and progression of Huntington’s disease (HD). However, any alterations in the fecal microbiome of HD patients and its relation to the host cytokine response remain unknown. The present study investigated alteratio...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-02-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2020.603594/full |
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| author | Gang Du Gang Du Wei Dong Wei Dong Qing Yang Xueying Yu Jinghong Ma Weihong Gu Yue Huang Yue Huang Yue Huang |
| author_facet | Gang Du Gang Du Wei Dong Wei Dong Qing Yang Xueying Yu Jinghong Ma Weihong Gu Yue Huang Yue Huang Yue Huang |
| author_sort | Gang Du |
| collection | DOAJ |
| description | Emerging evidence indicates that gut dysbiosis may play a regulatory role in the onset and progression of Huntington’s disease (HD). However, any alterations in the fecal microbiome of HD patients and its relation to the host cytokine response remain unknown. The present study investigated alterations and host cytokine responses in patients with HD. We enrolled 33 HD patients and 33 sex- and age- matched healthy controls. Fecal microbiota communities were determined through 16S ribosomal DNA gene sequencing, from which we analyzed fecal microbial richness, evenness, structure, and differential abundance of individual taxa between HD patients and healthy controls. HD patients were evaluated for their clinical characteristics, and the relationships of fecal microbiota with these clinical characteristics were analyzed. Plasma concentrations of interferon gamma (IFN-γ), interleukin 1 beta (IL-1β), IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, and tumor necrosis factor alpha were measured by Meso Scale Discovery (MSD) assays, and relationships between microbiota and cytokine levels were analyzed in the HD group. HD patients showed increased α-diversity (richness), β-diversity (structure), and altered relative abundances of several taxa compared to those in healthy controls. HD-associated clinical characteristics correlated with the abundances of components of fecal microbiota at the genus level. Genus Intestinimonas was correlated with total functional capacity scores and IL-4 levels. Our present study also revealed that genus Bilophila were negatively correlated with proinflammatory IL-6 levels. Taken together, our present study represents the first to demonstrate alterations in fecal microbiota and inflammatory cytokine responses in HD patients. Further elucidation of interactions between microbial and host immune responses may help to better understand the pathogenesis of HD. |
| first_indexed | 2024-12-20T03:29:15Z |
| format | Article |
| id | doaj.art-8dd335f27fc24e629afc9ea23ce26f73 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-20T03:29:15Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-8dd335f27fc24e629afc9ea23ce26f732022-12-21T19:55:01ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-02-011110.3389/fimmu.2020.603594603594Altered Gut Microbiota Related to Inflammatory Responses in Patients With Huntington’s DiseaseGang Du0Gang Du1Wei Dong2Wei Dong3Qing Yang4Xueying Yu5Jinghong Ma6Weihong Gu7Yue Huang8Yue Huang9Yue Huang10China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaCentre for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaChina National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaCentre for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaChina National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaCentre for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaNeurology Department, XuanWu Hospital, Capital Medical University, Beijing, ChinaNeurology Department, China-Japan Friendship Hospital, Beijing, ChinaChina National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaCentre for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaSchool of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, AustraliaEmerging evidence indicates that gut dysbiosis may play a regulatory role in the onset and progression of Huntington’s disease (HD). However, any alterations in the fecal microbiome of HD patients and its relation to the host cytokine response remain unknown. The present study investigated alterations and host cytokine responses in patients with HD. We enrolled 33 HD patients and 33 sex- and age- matched healthy controls. Fecal microbiota communities were determined through 16S ribosomal DNA gene sequencing, from which we analyzed fecal microbial richness, evenness, structure, and differential abundance of individual taxa between HD patients and healthy controls. HD patients were evaluated for their clinical characteristics, and the relationships of fecal microbiota with these clinical characteristics were analyzed. Plasma concentrations of interferon gamma (IFN-γ), interleukin 1 beta (IL-1β), IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, and tumor necrosis factor alpha were measured by Meso Scale Discovery (MSD) assays, and relationships between microbiota and cytokine levels were analyzed in the HD group. HD patients showed increased α-diversity (richness), β-diversity (structure), and altered relative abundances of several taxa compared to those in healthy controls. HD-associated clinical characteristics correlated with the abundances of components of fecal microbiota at the genus level. Genus Intestinimonas was correlated with total functional capacity scores and IL-4 levels. Our present study also revealed that genus Bilophila were negatively correlated with proinflammatory IL-6 levels. Taken together, our present study represents the first to demonstrate alterations in fecal microbiota and inflammatory cytokine responses in HD patients. Further elucidation of interactions between microbial and host immune responses may help to better understand the pathogenesis of HD.https://www.frontiersin.org/articles/10.3389/fimmu.2020.603594/fullHuntington’s diseasegut microbiota16S rDNAcytokinesneuroinflammation |
| spellingShingle | Gang Du Gang Du Wei Dong Wei Dong Qing Yang Xueying Yu Jinghong Ma Weihong Gu Yue Huang Yue Huang Yue Huang Altered Gut Microbiota Related to Inflammatory Responses in Patients With Huntington’s Disease Frontiers in Immunology Huntington’s disease gut microbiota 16S rDNA cytokines neuroinflammation |
| title | Altered Gut Microbiota Related to Inflammatory Responses in Patients With Huntington’s Disease |
| title_full | Altered Gut Microbiota Related to Inflammatory Responses in Patients With Huntington’s Disease |
| title_fullStr | Altered Gut Microbiota Related to Inflammatory Responses in Patients With Huntington’s Disease |
| title_full_unstemmed | Altered Gut Microbiota Related to Inflammatory Responses in Patients With Huntington’s Disease |
| title_short | Altered Gut Microbiota Related to Inflammatory Responses in Patients With Huntington’s Disease |
| title_sort | altered gut microbiota related to inflammatory responses in patients with huntington s disease |
| topic | Huntington’s disease gut microbiota 16S rDNA cytokines neuroinflammation |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2020.603594/full |
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