Anti-Inflammatory Effects Exerted by 14-Methoxyalternate C from Antarctic Fungal Strain <i>Pleosporales</i> sp. SF-7343 via the Regulation of NF-κB and JAK2/STAT3 in HaCaT Human Keratinocytes
Atopic dermatitis (AD) is a chronic inflammatory skin disease with a profound negative impact on patients’ quality of life. Four known secondary fungal metabolites were found in the chemical study of the Antarctic fungus <i>Pleosporales</i> sp. SF-7343, including 14-methoxyalternate C (&...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| author | Linsha Dong Thao Quyen Cao Zhiming Liu Nguyen Quoc Tuan Youn-Chul Kim Jae Hak Sohn Joung Han Yim Dong-Sung Lee Hyuncheol Oh |
| author_facet | Linsha Dong Thao Quyen Cao Zhiming Liu Nguyen Quoc Tuan Youn-Chul Kim Jae Hak Sohn Joung Han Yim Dong-Sung Lee Hyuncheol Oh |
| author_sort | Linsha Dong |
| collection | DOAJ |
| description | Atopic dermatitis (AD) is a chronic inflammatory skin disease with a profound negative impact on patients’ quality of life. Four known secondary fungal metabolites were found in the chemical study of the Antarctic fungus <i>Pleosporales</i> sp. SF-7343, including 14-methoxyalternate C (<b>1</b>), 5′-methoxy-6-methyl-biphenyl-3,4,3′-triol (<b>2</b>), 3,8,10-trihydroxy-4-methoxy-6-methylbenzocoumarin (<b>3</b>), and alternariol monomethyl ether (<b>4</b>). Additionally, we identified the skin anti-inflammatory composition from the SF-7343 strain. Interleukin-8 and -6 Screening results showed that compound <b>1</b> inhibited IL-8 and IL-6 in tumor necrosis factor-α/interferon-γ stimulated HaCaT cells. Compound <b>1</b> showed inhibitory effects on MDC and RANTES. It also downregulated the expression of intercellular adhesion molecule-1 (ICAM-1) and upregulated the expression of involucrin. The results of the mechanistic study showed that compound <b>1</b> inhibited the nuclear translocation of nuclear factor-kappa B p65 and STAT3. In conclusion, this study demonstrates the potential of the Antarctic fungal strain SF-7343 as a bioactive resource to inhibit skin inflammation, such as AD. |
| first_indexed | 2024-03-09T17:46:38Z |
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| last_indexed | 2024-03-09T17:46:38Z |
| publishDate | 2022-11-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8dd76bb74be84d0a82b958cdf3af91492023-11-24T11:05:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123231464210.3390/ijms232314642Anti-Inflammatory Effects Exerted by 14-Methoxyalternate C from Antarctic Fungal Strain <i>Pleosporales</i> sp. SF-7343 via the Regulation of NF-κB and JAK2/STAT3 in HaCaT Human KeratinocytesLinsha Dong0Thao Quyen Cao1Zhiming Liu2Nguyen Quoc Tuan3Youn-Chul Kim4Jae Hak Sohn5Joung Han Yim6Dong-Sung Lee7Hyuncheol Oh8College of Pharmacy, Chosun University, Dong-gu, Gwangju 61452, Republic of KoreaInstitute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 54538, Republic of KoreaCollege of Pharmacy, Chosun University, Dong-gu, Gwangju 61452, Republic of KoreaInstitute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 54538, Republic of KoreaInstitute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 54538, Republic of KoreaCollege of Medical and Life Sciences, Silla University, Busan 46958, Republic of KoreaDivision of Polar Life Sciences, Korea Polar Research Institute, Incheon 21990, Republic of KoreaCollege of Pharmacy, Chosun University, Dong-gu, Gwangju 61452, Republic of KoreaInstitute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 54538, Republic of KoreaAtopic dermatitis (AD) is a chronic inflammatory skin disease with a profound negative impact on patients’ quality of life. Four known secondary fungal metabolites were found in the chemical study of the Antarctic fungus <i>Pleosporales</i> sp. SF-7343, including 14-methoxyalternate C (<b>1</b>), 5′-methoxy-6-methyl-biphenyl-3,4,3′-triol (<b>2</b>), 3,8,10-trihydroxy-4-methoxy-6-methylbenzocoumarin (<b>3</b>), and alternariol monomethyl ether (<b>4</b>). Additionally, we identified the skin anti-inflammatory composition from the SF-7343 strain. Interleukin-8 and -6 Screening results showed that compound <b>1</b> inhibited IL-8 and IL-6 in tumor necrosis factor-α/interferon-γ stimulated HaCaT cells. Compound <b>1</b> showed inhibitory effects on MDC and RANTES. It also downregulated the expression of intercellular adhesion molecule-1 (ICAM-1) and upregulated the expression of involucrin. The results of the mechanistic study showed that compound <b>1</b> inhibited the nuclear translocation of nuclear factor-kappa B p65 and STAT3. In conclusion, this study demonstrates the potential of the Antarctic fungal strain SF-7343 as a bioactive resource to inhibit skin inflammation, such as AD.https://www.mdpi.com/1422-0067/23/23/14642Antarctic fungiskin inflammationJAK2/STAT3NF-κBHaCaT |
| spellingShingle | Linsha Dong Thao Quyen Cao Zhiming Liu Nguyen Quoc Tuan Youn-Chul Kim Jae Hak Sohn Joung Han Yim Dong-Sung Lee Hyuncheol Oh Anti-Inflammatory Effects Exerted by 14-Methoxyalternate C from Antarctic Fungal Strain <i>Pleosporales</i> sp. SF-7343 via the Regulation of NF-κB and JAK2/STAT3 in HaCaT Human Keratinocytes International Journal of Molecular Sciences Antarctic fungi skin inflammation JAK2/STAT3 NF-κB HaCaT |
| title | Anti-Inflammatory Effects Exerted by 14-Methoxyalternate C from Antarctic Fungal Strain <i>Pleosporales</i> sp. SF-7343 via the Regulation of NF-κB and JAK2/STAT3 in HaCaT Human Keratinocytes |
| title_full | Anti-Inflammatory Effects Exerted by 14-Methoxyalternate C from Antarctic Fungal Strain <i>Pleosporales</i> sp. SF-7343 via the Regulation of NF-κB and JAK2/STAT3 in HaCaT Human Keratinocytes |
| title_fullStr | Anti-Inflammatory Effects Exerted by 14-Methoxyalternate C from Antarctic Fungal Strain <i>Pleosporales</i> sp. SF-7343 via the Regulation of NF-κB and JAK2/STAT3 in HaCaT Human Keratinocytes |
| title_full_unstemmed | Anti-Inflammatory Effects Exerted by 14-Methoxyalternate C from Antarctic Fungal Strain <i>Pleosporales</i> sp. SF-7343 via the Regulation of NF-κB and JAK2/STAT3 in HaCaT Human Keratinocytes |
| title_short | Anti-Inflammatory Effects Exerted by 14-Methoxyalternate C from Antarctic Fungal Strain <i>Pleosporales</i> sp. SF-7343 via the Regulation of NF-κB and JAK2/STAT3 in HaCaT Human Keratinocytes |
| title_sort | anti inflammatory effects exerted by 14 methoxyalternate c from antarctic fungal strain i pleosporales i sp sf 7343 via the regulation of nf κb and jak2 stat3 in hacat human keratinocytes |
| topic | Antarctic fungi skin inflammation JAK2/STAT3 NF-κB HaCaT |
| url | https://www.mdpi.com/1422-0067/23/23/14642 |
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