How Structured Metadata Acquisition Contributes to the Reproducibility of Nanosafety Studies: Evaluation by a Round-Robin Test
It has been widely recognized that nanosafety studies are limited in reproducibility, caused by missing or inadequate information and data gaps. Reliable and comprehensive studies should be performed supported by standards or guidelines, which need to be harmonized and usable for the multidisciplina...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/7/1053 |
| _version_ | 1797438326286843904 |
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| author | Linda Elberskirch Adriana Sofranko Julia Liebing Norbert Riefler Kunigunde Binder Christian Bonatto Minella Matthias Razum Lutz Mädler Klaus Unfried Roel P. F. Schins Annette Kraegeloh Christoph van Thriel |
| author_facet | Linda Elberskirch Adriana Sofranko Julia Liebing Norbert Riefler Kunigunde Binder Christian Bonatto Minella Matthias Razum Lutz Mädler Klaus Unfried Roel P. F. Schins Annette Kraegeloh Christoph van Thriel |
| author_sort | Linda Elberskirch |
| collection | DOAJ |
| description | It has been widely recognized that nanosafety studies are limited in reproducibility, caused by missing or inadequate information and data gaps. Reliable and comprehensive studies should be performed supported by standards or guidelines, which need to be harmonized and usable for the multidisciplinary field of nanosafety research. The previously described minimal information table (MIT), based on existing standards or guidelines, represents one approach towards harmonization. Here, we demonstrate the applicability and advantages of the MIT by a round-robin test. Its modular structure enables describing individual studies comprehensively by a combination of various relevant aspects. Three laboratories conducted a WST-1 cell viability assay using A549 cells to analyze the effects of the reference nanomaterials NM101 and NM110 according to predefined (S)OPs. The MIT contains relevant and defined descriptive information and quality criteria and thus supported the implementation of the round-robin test from planning, investigation to analysis and data interpretation. As a result, we could identify sources of variability and justify deviating results attributed to differences in specific procedures. Consequently, the use of the MIT contributes to the acquisition of reliable and comprehensive datasets and therefore improves the significance and reusability of nanosafety studies. |
| first_indexed | 2024-03-09T11:35:06Z |
| format | Article |
| id | doaj.art-8dd9b552a0d54a179664af8cfbaa422d |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T11:35:06Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-8dd9b552a0d54a179664af8cfbaa422d2023-11-30T23:43:42ZengMDPI AGNanomaterials2079-49912022-03-01127105310.3390/nano12071053How Structured Metadata Acquisition Contributes to the Reproducibility of Nanosafety Studies: Evaluation by a Round-Robin TestLinda Elberskirch0Adriana Sofranko1Julia Liebing2Norbert Riefler3Kunigunde Binder4Christian Bonatto Minella5Matthias Razum6Lutz Mädler7Klaus Unfried8Roel P. F. Schins9Annette Kraegeloh10Christoph van Thriel11INM—Leibniz Institute for New Materials, Campus D2 2, 66123 Saarbrücken, GermanyIUF—Leibniz Research Institute for Environmental Medicine, Auf’m Hennekamp 50, 40225 Düsseldorf, GermanyIfADo—Leibniz Research Centre for Working Environment and Human Factors, Ardeystraße 67, 44139 Dortmund, GermanyIWT—Leibniz-Institut für Werkstofforientierte Technologien, Badgasteiner Str. 3, 28359 Bremen, GermanyFIZ Karlsruhe—Leibniz Institute for Information Infrastructure, Hermann-von-Helmholtz-Platz 1, 76133 Eggenstein-Leopoldshafen, GermanyFIZ Karlsruhe—Leibniz Institute for Information Infrastructure, Hermann-von-Helmholtz-Platz 1, 76133 Eggenstein-Leopoldshafen, GermanyFIZ Karlsruhe—Leibniz Institute for Information Infrastructure, Hermann-von-Helmholtz-Platz 1, 76133 Eggenstein-Leopoldshafen, GermanyIWT—Leibniz-Institut für Werkstofforientierte Technologien, Badgasteiner Str. 3, 28359 Bremen, GermanyIUF—Leibniz Research Institute for Environmental Medicine, Auf’m Hennekamp 50, 40225 Düsseldorf, GermanyIUF—Leibniz Research Institute for Environmental Medicine, Auf’m Hennekamp 50, 40225 Düsseldorf, GermanyINM—Leibniz Institute for New Materials, Campus D2 2, 66123 Saarbrücken, GermanyIfADo—Leibniz Research Centre for Working Environment and Human Factors, Ardeystraße 67, 44139 Dortmund, GermanyIt has been widely recognized that nanosafety studies are limited in reproducibility, caused by missing or inadequate information and data gaps. Reliable and comprehensive studies should be performed supported by standards or guidelines, which need to be harmonized and usable for the multidisciplinary field of nanosafety research. The previously described minimal information table (MIT), based on existing standards or guidelines, represents one approach towards harmonization. Here, we demonstrate the applicability and advantages of the MIT by a round-robin test. Its modular structure enables describing individual studies comprehensively by a combination of various relevant aspects. Three laboratories conducted a WST-1 cell viability assay using A549 cells to analyze the effects of the reference nanomaterials NM101 and NM110 according to predefined (S)OPs. The MIT contains relevant and defined descriptive information and quality criteria and thus supported the implementation of the round-robin test from planning, investigation to analysis and data interpretation. As a result, we could identify sources of variability and justify deviating results attributed to differences in specific procedures. Consequently, the use of the MIT contributes to the acquisition of reliable and comprehensive datasets and therefore improves the significance and reusability of nanosafety studies.https://www.mdpi.com/2079-4991/12/7/1053interlaboratory comparisonminimal informationquality criteriadescription standards |
| spellingShingle | Linda Elberskirch Adriana Sofranko Julia Liebing Norbert Riefler Kunigunde Binder Christian Bonatto Minella Matthias Razum Lutz Mädler Klaus Unfried Roel P. F. Schins Annette Kraegeloh Christoph van Thriel How Structured Metadata Acquisition Contributes to the Reproducibility of Nanosafety Studies: Evaluation by a Round-Robin Test Nanomaterials interlaboratory comparison minimal information quality criteria description standards |
| title | How Structured Metadata Acquisition Contributes to the Reproducibility of Nanosafety Studies: Evaluation by a Round-Robin Test |
| title_full | How Structured Metadata Acquisition Contributes to the Reproducibility of Nanosafety Studies: Evaluation by a Round-Robin Test |
| title_fullStr | How Structured Metadata Acquisition Contributes to the Reproducibility of Nanosafety Studies: Evaluation by a Round-Robin Test |
| title_full_unstemmed | How Structured Metadata Acquisition Contributes to the Reproducibility of Nanosafety Studies: Evaluation by a Round-Robin Test |
| title_short | How Structured Metadata Acquisition Contributes to the Reproducibility of Nanosafety Studies: Evaluation by a Round-Robin Test |
| title_sort | how structured metadata acquisition contributes to the reproducibility of nanosafety studies evaluation by a round robin test |
| topic | interlaboratory comparison minimal information quality criteria description standards |
| url | https://www.mdpi.com/2079-4991/12/7/1053 |
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