Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein
SUMMARY: Turn-on fluorescent probe mediated by conjugate addition and cyclization (TCC probe) is a small molecule that reacts with a protein of interest in cells. TCC probe is applicable to various types of proteins by exchanging the ligand unit for target proteins. TCC probes are a potent tool for...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-06-01
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| Series: | STAR Protocols |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672030023X |
| _version_ | 1831648116794720256 |
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| author | Hiroyuki Kojima Toshimasa Itoh |
| author_facet | Hiroyuki Kojima Toshimasa Itoh |
| author_sort | Hiroyuki Kojima |
| collection | DOAJ |
| description | SUMMARY: Turn-on fluorescent probe mediated by conjugate addition and cyclization (TCC probe) is a small molecule that reacts with a protein of interest in cells. TCC probe is applicable to various types of proteins by exchanging the ligand unit for target proteins. TCC probes are a potent tool for molecular imaging and chemical proteomics. This protocol describes the synthesis of a TCC probe via unstable intermediate and how to use this probe to visualize vitamin D receptor as a target protein.For complete details on the use and execution of this protocol, please refer to Kojima et al. (2020). |
| first_indexed | 2024-12-19T14:26:28Z |
| format | Article |
| id | doaj.art-8ddb285aa9de4c6fb98c6afba2ff0545 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-19T14:26:28Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-8ddb285aa9de4c6fb98c6afba2ff05452022-12-21T20:17:36ZengElsevierSTAR Protocols2666-16672020-06-0111100036Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target ProteinHiroyuki Kojima0Toshimasa Itoh1Showa Pharmaceutical University, 3-3165 Higashi-Tamagawagakuen, Machida, Tokyo 194-8543, Japan; Corresponding authorShowa Pharmaceutical University, 3-3165 Higashi-Tamagawagakuen, Machida, Tokyo 194-8543, JapanSUMMARY: Turn-on fluorescent probe mediated by conjugate addition and cyclization (TCC probe) is a small molecule that reacts with a protein of interest in cells. TCC probe is applicable to various types of proteins by exchanging the ligand unit for target proteins. TCC probes are a potent tool for molecular imaging and chemical proteomics. This protocol describes the synthesis of a TCC probe via unstable intermediate and how to use this probe to visualize vitamin D receptor as a target protein.For complete details on the use and execution of this protocol, please refer to Kojima et al. (2020).http://www.sciencedirect.com/science/article/pii/S266616672030023X |
| spellingShingle | Hiroyuki Kojima Toshimasa Itoh Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein STAR Protocols |
| title | Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein |
| title_full | Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein |
| title_fullStr | Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein |
| title_full_unstemmed | Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein |
| title_short | Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein |
| title_sort | preparation and use of turn on fluorescent probe for detection and live cell imaging of vitamin d receptor as a target protein |
| url | http://www.sciencedirect.com/science/article/pii/S266616672030023X |
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