Innate immune response in bovine neutrophils stimulated with Mycoplasma bovis
Abstract Mycoplasma bovis (M. bovis) is a significant worldwide pathogen of cattle. Neutrophils have an important role in the innate immune response during infection with M. bovis. However, even though neutrophils accumulate in M. bovis infection, the interaction of M. bovis and neutrophils has not...
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| Format: | Article |
| Language: | English |
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BMC
2021-04-01
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| Series: | Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s13567-021-00920-2 |
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| author | Satoshi Gondaira Koji Nishi Jumpei Fujiki Hidetomo Iwano Reina Watanabe Ayako Eguchi Yuki Hirano Hidetoshi Higuchi Hajime Nagahata |
| author_facet | Satoshi Gondaira Koji Nishi Jumpei Fujiki Hidetomo Iwano Reina Watanabe Ayako Eguchi Yuki Hirano Hidetoshi Higuchi Hajime Nagahata |
| author_sort | Satoshi Gondaira |
| collection | DOAJ |
| description | Abstract Mycoplasma bovis (M. bovis) is a significant worldwide pathogen of cattle. Neutrophils have an important role in the innate immune response during infection with M. bovis. However, even though neutrophils accumulate in M. bovis infection, the interaction of M. bovis and neutrophils has not been fully elucidated. We attempted to elucidate the innate immune response of neutrophils stimulated with M. bovis and evaluate the transcriptome and functional analysis of bovine neutrophils stimulated with M. bovis. Proinflammatory cytokines, such as inducible nitric oxide (iNOS), which was the most increased gene in transcriptome analysis, were increased in quantitative polymerase chain reaction analysis of bovine neutrophils stimulated with live or heat-killed M. bovis. Nitric oxide and intracellular reactive oxygen species production of neutrophils stimulated with M. bovis was significantly increased. Neutrophils stimulated with M. bovis showed an increased ratio of nonapoptotic cell death compared to unstimulated controls. We demonstrated that neutrophil extracellular traps (NETs) formation was not recognized in neutrophils stimulated with live M. bovis. However, heat-killed M. bovis induced NETs formation. We also showed the interaction with M. bovis and bovine neutrophils regarding proinflammatory cytokine gene expression and functional expression related to NETs formation. Live and killed M. bovis induced innate immune responses in neutrophils and had the potential to induce NETs formation, but live M. bovis escaped NETs. |
| first_indexed | 2024-12-14T22:25:17Z |
| format | Article |
| id | doaj.art-8ddcb3337aa141579beedb5632bd7079 |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-12-14T22:25:17Z |
| publishDate | 2021-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-8ddcb3337aa141579beedb5632bd70792022-12-21T22:45:23ZengBMCVeterinary Research1297-97162021-04-0152111110.1186/s13567-021-00920-2Innate immune response in bovine neutrophils stimulated with Mycoplasma bovisSatoshi Gondaira0Koji Nishi1Jumpei Fujiki2Hidetomo Iwano3Reina Watanabe4Ayako Eguchi5Yuki Hirano6Hidetoshi Higuchi7Hajime Nagahata8Animal Health Laboratory, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen UniversityAnimal Health Laboratory, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen UniversityVeterinary Biochemistry, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen UniversityVeterinary Biochemistry, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen UniversityAnimal Health Laboratory, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen UniversityAnimal Health Laboratory, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen UniversityAnimal Research Center, Agricultural Research Department, Hokkaido Research OrganizationAnimal Health Laboratory, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen UniversityAnimal Health Laboratory, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen UniversityAbstract Mycoplasma bovis (M. bovis) is a significant worldwide pathogen of cattle. Neutrophils have an important role in the innate immune response during infection with M. bovis. However, even though neutrophils accumulate in M. bovis infection, the interaction of M. bovis and neutrophils has not been fully elucidated. We attempted to elucidate the innate immune response of neutrophils stimulated with M. bovis and evaluate the transcriptome and functional analysis of bovine neutrophils stimulated with M. bovis. Proinflammatory cytokines, such as inducible nitric oxide (iNOS), which was the most increased gene in transcriptome analysis, were increased in quantitative polymerase chain reaction analysis of bovine neutrophils stimulated with live or heat-killed M. bovis. Nitric oxide and intracellular reactive oxygen species production of neutrophils stimulated with M. bovis was significantly increased. Neutrophils stimulated with M. bovis showed an increased ratio of nonapoptotic cell death compared to unstimulated controls. We demonstrated that neutrophil extracellular traps (NETs) formation was not recognized in neutrophils stimulated with live M. bovis. However, heat-killed M. bovis induced NETs formation. We also showed the interaction with M. bovis and bovine neutrophils regarding proinflammatory cytokine gene expression and functional expression related to NETs formation. Live and killed M. bovis induced innate immune responses in neutrophils and had the potential to induce NETs formation, but live M. bovis escaped NETs.https://doi.org/10.1186/s13567-021-00920-2Mycoplasma bovisBovineNeutrophilsInnate immuneTranscriptome analysis |
| spellingShingle | Satoshi Gondaira Koji Nishi Jumpei Fujiki Hidetomo Iwano Reina Watanabe Ayako Eguchi Yuki Hirano Hidetoshi Higuchi Hajime Nagahata Innate immune response in bovine neutrophils stimulated with Mycoplasma bovis Veterinary Research Mycoplasma bovis Bovine Neutrophils Innate immune Transcriptome analysis |
| title | Innate immune response in bovine neutrophils stimulated with Mycoplasma bovis |
| title_full | Innate immune response in bovine neutrophils stimulated with Mycoplasma bovis |
| title_fullStr | Innate immune response in bovine neutrophils stimulated with Mycoplasma bovis |
| title_full_unstemmed | Innate immune response in bovine neutrophils stimulated with Mycoplasma bovis |
| title_short | Innate immune response in bovine neutrophils stimulated with Mycoplasma bovis |
| title_sort | innate immune response in bovine neutrophils stimulated with mycoplasma bovis |
| topic | Mycoplasma bovis Bovine Neutrophils Innate immune Transcriptome analysis |
| url | https://doi.org/10.1186/s13567-021-00920-2 |
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