Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor
We constructed an original supramolecular assembly on a surface of sensor composed of an innovative combination of an engineered cytochrome b5 and a modified nucleic acid bound to a synthetic lipid hemimembrane. The protein/DNA block, called (PDNA) 2, was synthesized and purified before its immobili...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2008-07-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/8/7/4413/ |
| _version_ | 1797999334750420992 |
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| author | Wilfrid Boireau Régis Delage-Mourroux Eric Lesniewska Céline Elie-Caille Alexandre Berthier |
| author_facet | Wilfrid Boireau Régis Delage-Mourroux Eric Lesniewska Céline Elie-Caille Alexandre Berthier |
| author_sort | Wilfrid Boireau |
| collection | DOAJ |
| description | We constructed an original supramolecular assembly on a surface of sensor composed of an innovative combination of an engineered cytochrome b5 and a modified nucleic acid bound to a synthetic lipid hemimembrane. The protein/DNA block, called (PDNA) 2, was synthesized and purified before its immobilization onto a hybrid bilayer reconstituted on a gold surface. Surface plasmon resonance (SPR) and atomic force microscopy (AFM) were engaged in parallel on the same substrates in order to better understand dynamic events that occur at the surface of the biosensor. Good correlations were obtained in terms of specificity and reversibility. These findings allow us to present a first application of such biosensor in the study of the interaction processes between nuclear receptor and DNA. |
| first_indexed | 2024-04-11T11:03:07Z |
| format | Article |
| id | doaj.art-8ddf0d5d62ce450b9d028f574aaf3eab |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T11:03:07Z |
| publishDate | 2008-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-8ddf0d5d62ce450b9d028f574aaf3eab2022-12-22T04:28:27ZengMDPI AGSensors1424-82202008-07-018744134428Nanobioengineering and Characterization of a Novel Estrogen Receptor BiosensorWilfrid BoireauRégis Delage-MourrouxEric LesniewskaCéline Elie-CailleAlexandre BerthierWe constructed an original supramolecular assembly on a surface of sensor composed of an innovative combination of an engineered cytochrome b5 and a modified nucleic acid bound to a synthetic lipid hemimembrane. The protein/DNA block, called (PDNA) 2, was synthesized and purified before its immobilization onto a hybrid bilayer reconstituted on a gold surface. Surface plasmon resonance (SPR) and atomic force microscopy (AFM) were engaged in parallel on the same substrates in order to better understand dynamic events that occur at the surface of the biosensor. Good correlations were obtained in terms of specificity and reversibility. These findings allow us to present a first application of such biosensor in the study of the interaction processes between nuclear receptor and DNA.http://www.mdpi.com/1424-8220/8/7/4413/Nano-objectsmolecular legoAFMSPRProtein/DNA interaction. |
| spellingShingle | Wilfrid Boireau Régis Delage-Mourroux Eric Lesniewska Céline Elie-Caille Alexandre Berthier Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor Sensors Nano-objects molecular lego AFM SPR Protein/DNA interaction. |
| title | Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor |
| title_full | Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor |
| title_fullStr | Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor |
| title_full_unstemmed | Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor |
| title_short | Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor |
| title_sort | nanobioengineering and characterization of a novel estrogen receptor biosensor |
| topic | Nano-objects molecular lego AFM SPR Protein/DNA interaction. |
| url | http://www.mdpi.com/1424-8220/8/7/4413/ |
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