Identification and characterization of immunogenic genes from genomic expression library of mycoplasma ovipneumoniae
Mycoplasma ovipneumoniae is an important pathogen causing respiratory disease in sheep. At present, the immune-associated antigens of M. ovipneumoniae are still unknown, which significantly limits the development of new vaccines for M. ovipneumoniae. In order to identify and characterize the immune-...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2019-10-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2654 |
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| author | Mengfan QIAO Cheng CHEN Sufang YANG Xingxing ZHANG Lulu TIAN Haiting LU Shasha GONG Xuepeng CAI Qingling MENG Jun QIAO |
| author_facet | Mengfan QIAO Cheng CHEN Sufang YANG Xingxing ZHANG Lulu TIAN Haiting LU Shasha GONG Xuepeng CAI Qingling MENG Jun QIAO |
| author_sort | Mengfan QIAO |
| collection | DOAJ |
| description | Mycoplasma ovipneumoniae is an important pathogen causing respiratory disease in sheep. At present, the immune-associated antigens
of M. ovipneumoniae are still unknown, which significantly limits the development of new vaccines for M. ovipneumoniae. In order to
identify and characterize the immune-associated antigen genes, genomic expression library of M. ovipneumoniae was constructed and
identified, from which positive clones were recognized and screened by positive serum against M. ovipneumoniae. Sequence analysis
showed that these 10 clones contained 5 different genes encoding P97-like protein, P102-like protein, Translation initiation factor (IF-1),
Methionine aminopeptidase (MAP) and P56 membrane protein, respectively. Three proteins including IF-1, MAP and P97-like protein
were expressed in E. coli and used to immunize lambs to verify their immunogenicity, respectively. Animal immunization test confirmed
that the novel protein MAP displayed a strong immunogenicity, while the immunogenicity of P97-like protein and IF-1 were relatively
weak. The identification of immunogenic protein MAP provided a potentially valuable antigen candidate for the development of
serological diagnostic method and subunit vaccine against M. ovipneumoniae infection. |
| first_indexed | 2024-03-13T04:38:27Z |
| format | Article |
| id | doaj.art-8de2404944304bd6a77fe527fc222e15 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:38:27Z |
| publishDate | 2019-10-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-8de2404944304bd6a77fe527fc222e152023-06-19T06:33:03ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512019-10-0126220120710.9775/kvfd.2019.227302654Identification and characterization of immunogenic genes from genomic expression library of mycoplasma ovipneumoniaeMengfan QIAO0Cheng CHEN1Sufang YANG2Xingxing ZHANG3Lulu TIAN4Haiting LU5Shasha GONG6Xuepeng CAI7Qingling MENG8Jun QIAO9College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, CHINASchool of Biotechnology, Bayingol Vocational and Technical College, Korla, Xinjiang, 841000, CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, CHINAState Key Lab of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, CHINAMycoplasma ovipneumoniae is an important pathogen causing respiratory disease in sheep. At present, the immune-associated antigens of M. ovipneumoniae are still unknown, which significantly limits the development of new vaccines for M. ovipneumoniae. In order to identify and characterize the immune-associated antigen genes, genomic expression library of M. ovipneumoniae was constructed and identified, from which positive clones were recognized and screened by positive serum against M. ovipneumoniae. Sequence analysis showed that these 10 clones contained 5 different genes encoding P97-like protein, P102-like protein, Translation initiation factor (IF-1), Methionine aminopeptidase (MAP) and P56 membrane protein, respectively. Three proteins including IF-1, MAP and P97-like protein were expressed in E. coli and used to immunize lambs to verify their immunogenicity, respectively. Animal immunization test confirmed that the novel protein MAP displayed a strong immunogenicity, while the immunogenicity of P97-like protein and IF-1 were relatively weak. The identification of immunogenic protein MAP provided a potentially valuable antigen candidate for the development of serological diagnostic method and subunit vaccine against M. ovipneumoniae infection.https://vetdergikafkas.org/pdf.php?id=2654mycoplasma ovipneumoniaeimmune-associated antigenscreeningcharacterization |
| spellingShingle | Mengfan QIAO Cheng CHEN Sufang YANG Xingxing ZHANG Lulu TIAN Haiting LU Shasha GONG Xuepeng CAI Qingling MENG Jun QIAO Identification and characterization of immunogenic genes from genomic expression library of mycoplasma ovipneumoniae Kafkas Universitesi Veteriner Fakültesi Dergisi mycoplasma ovipneumoniae immune-associated antigen screening characterization |
| title | Identification and characterization of immunogenic genes from genomic expression library of mycoplasma ovipneumoniae |
| title_full | Identification and characterization of immunogenic genes from genomic expression library of mycoplasma ovipneumoniae |
| title_fullStr | Identification and characterization of immunogenic genes from genomic expression library of mycoplasma ovipneumoniae |
| title_full_unstemmed | Identification and characterization of immunogenic genes from genomic expression library of mycoplasma ovipneumoniae |
| title_short | Identification and characterization of immunogenic genes from genomic expression library of mycoplasma ovipneumoniae |
| title_sort | identification and characterization of immunogenic genes from genomic expression library of mycoplasma ovipneumoniae |
| topic | mycoplasma ovipneumoniae immune-associated antigen screening characterization |
| url | https://vetdergikafkas.org/pdf.php?id=2654 |
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