Application of a Validated Method for the Identification and Quantification of Mycotoxins in Wines Using UPLC-MS/MS
The aim of the present study was to develop a rapid, simple and reliable method for the identification and quantification of six mycotoxins in wine using liquid chromatography with electrospray ionization tandem mass spectrometry. The analytical method was fully validated, and calibration curves wer...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Separations |
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| Online Access: | https://www.mdpi.com/2297-8739/9/4/102 |
| _version_ | 1797434233590906880 |
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| author | Artemis P. Louppis Michalis S. Constantinou |
| author_facet | Artemis P. Louppis Michalis S. Constantinou |
| author_sort | Artemis P. Louppis |
| collection | DOAJ |
| description | The aim of the present study was to develop a rapid, simple and reliable method for the identification and quantification of six mycotoxins in wine using liquid chromatography with electrospray ionization tandem mass spectrometry. The analytical method was fully validated, and calibration curves were made with correlation coefficients >0.9970. A short analysis time and acceptable extraction efficiency were achieved by a direct extraction method of analytes (ochratoxin A, aflatoxin B1, B2, G1, G2 and Zearalenone) with acetonitrile. LOD values were from 0.03 to 0.27 μg kg<sup>−1</sup>, and LOQ values were from 0.08 to 0.81 μg kg<sup>−1</sup>, with recoveries at various values from 77 to 108%. The expanded uncertainty was 5–21% expressed at a coverage level of k = 2, at a confidence level of approximately 95%. The performance criteria of the method were fully met according to European legislation (EC) 401/2006. The method was successfully applied to wine samples from Cyprus. The method was simple, low cost, quick, accurate, and sensitive. |
| first_indexed | 2024-03-09T10:29:15Z |
| format | Article |
| id | doaj.art-8de3f5736fb7450a97d8c2b053cd97ac |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-09T10:29:15Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-8de3f5736fb7450a97d8c2b053cd97ac2023-12-01T21:24:07ZengMDPI AGSeparations2297-87392022-04-019410210.3390/separations9040102Application of a Validated Method for the Identification and Quantification of Mycotoxins in Wines Using UPLC-MS/MSArtemis P. Louppis0Michalis S. Constantinou1Analytical Department, MC Analysis Center Ltd., Nicosia 2563, CyprusAnalytical Department, MC Analysis Center Ltd., Nicosia 2563, CyprusThe aim of the present study was to develop a rapid, simple and reliable method for the identification and quantification of six mycotoxins in wine using liquid chromatography with electrospray ionization tandem mass spectrometry. The analytical method was fully validated, and calibration curves were made with correlation coefficients >0.9970. A short analysis time and acceptable extraction efficiency were achieved by a direct extraction method of analytes (ochratoxin A, aflatoxin B1, B2, G1, G2 and Zearalenone) with acetonitrile. LOD values were from 0.03 to 0.27 μg kg<sup>−1</sup>, and LOQ values were from 0.08 to 0.81 μg kg<sup>−1</sup>, with recoveries at various values from 77 to 108%. The expanded uncertainty was 5–21% expressed at a coverage level of k = 2, at a confidence level of approximately 95%. The performance criteria of the method were fully met according to European legislation (EC) 401/2006. The method was successfully applied to wine samples from Cyprus. The method was simple, low cost, quick, accurate, and sensitive.https://www.mdpi.com/2297-8739/9/4/102mycotoxinsdeterminationUPLC–tandem mass spectrometrymethod validationapplication developmentwine |
| spellingShingle | Artemis P. Louppis Michalis S. Constantinou Application of a Validated Method for the Identification and Quantification of Mycotoxins in Wines Using UPLC-MS/MS Separations mycotoxins determination UPLC–tandem mass spectrometry method validation application development wine |
| title | Application of a Validated Method for the Identification and Quantification of Mycotoxins in Wines Using UPLC-MS/MS |
| title_full | Application of a Validated Method for the Identification and Quantification of Mycotoxins in Wines Using UPLC-MS/MS |
| title_fullStr | Application of a Validated Method for the Identification and Quantification of Mycotoxins in Wines Using UPLC-MS/MS |
| title_full_unstemmed | Application of a Validated Method for the Identification and Quantification of Mycotoxins in Wines Using UPLC-MS/MS |
| title_short | Application of a Validated Method for the Identification and Quantification of Mycotoxins in Wines Using UPLC-MS/MS |
| title_sort | application of a validated method for the identification and quantification of mycotoxins in wines using uplc ms ms |
| topic | mycotoxins determination UPLC–tandem mass spectrometry method validation application development wine |
| url | https://www.mdpi.com/2297-8739/9/4/102 |
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