Characterization and expression analysis of the WRKY gene family in moso bamboo
Abstract The WRKY family of transcription factors (TFs) is one of the ten largest families of TFs in higher plants and has been implicated in multiple biological processes. Here, we identified 121 WRKY TFs in moso bamboo, including five novel members that were not annotated in the Phyllostachys edul...
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Nature Portfolio
2017-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-06701-2 |
| _version_ | 1818751903042895872 |
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| author | Long Li Shaohua Mu Zhanchao Cheng Yuanwen Cheng Ying Zhang Ying Miao Chenglin Hou Xueping Li Jian Gao |
| author_facet | Long Li Shaohua Mu Zhanchao Cheng Yuanwen Cheng Ying Zhang Ying Miao Chenglin Hou Xueping Li Jian Gao |
| author_sort | Long Li |
| collection | DOAJ |
| description | Abstract The WRKY family of transcription factors (TFs) is one of the ten largest families of TFs in higher plants and has been implicated in multiple biological processes. Here, we identified 121 WRKY TFs in moso bamboo, including five novel members that were not annotated in the Phyllostachys edulis genomic database. Estimation of the divergence time of paralogous gene pairs revealed an important role of the recent whole-genome duplication in the expansion of the WRKY family. Expression analysis based on quantitative reverse-transcription polymerase chain reaction (qRT-PCR) data revealed that a large number of PheWRKY genes varied significantly under cold or drought stress treatments, which could be defined as abiotic stress-responsive genes. The overexpression of PheWRKY72-2 in Arabidopsis resulted in a decreased sensitivity to drought stress during early seedling growth. PheWRKY72-2 may enhance plant tolerance to stress by functioning as a positive regulator of stoma closure. Our study provides a theoretical foundation and some experimental evidence for further functional verification of the PheWRKY family of TFs. |
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| id | doaj.art-8dee4ed7a40f409283fa309633e1c986 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T04:42:57Z |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
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| spelling | doaj.art-8dee4ed7a40f409283fa309633e1c9862022-12-21T21:20:40ZengNature PortfolioScientific Reports2045-23222017-07-017111610.1038/s41598-017-06701-2Characterization and expression analysis of the WRKY gene family in moso bambooLong Li0Shaohua Mu1Zhanchao Cheng2Yuanwen Cheng3Ying Zhang4Ying Miao5Chenglin Hou6Xueping Li7Jian Gao8International Center for Bamboo and Rattan, Key Laboratory of Bamboo and Rattan Science and Technology, State Forestry AdministrationInternational Center for Bamboo and Rattan, Key Laboratory of Bamboo and Rattan Science and Technology, State Forestry AdministrationInternational Center for Bamboo and Rattan, Key Laboratory of Bamboo and Rattan Science and Technology, State Forestry AdministrationInternational Center for Bamboo and Rattan, Key Laboratory of Bamboo and Rattan Science and Technology, State Forestry AdministrationInternational Center for Bamboo and Rattan, Key Laboratory of Bamboo and Rattan Science and Technology, State Forestry AdministrationCenter for Molecular Cell and Systems Biology, College of Life Sciences, Fujian Agriculture and Forestry UniversityDepartment of Microbiology, College of Life Science, Capital Normal UniversityInternational Center for Bamboo and Rattan, Key Laboratory of Bamboo and Rattan Science and Technology, State Forestry AdministrationInternational Center for Bamboo and Rattan, Key Laboratory of Bamboo and Rattan Science and Technology, State Forestry AdministrationAbstract The WRKY family of transcription factors (TFs) is one of the ten largest families of TFs in higher plants and has been implicated in multiple biological processes. Here, we identified 121 WRKY TFs in moso bamboo, including five novel members that were not annotated in the Phyllostachys edulis genomic database. Estimation of the divergence time of paralogous gene pairs revealed an important role of the recent whole-genome duplication in the expansion of the WRKY family. Expression analysis based on quantitative reverse-transcription polymerase chain reaction (qRT-PCR) data revealed that a large number of PheWRKY genes varied significantly under cold or drought stress treatments, which could be defined as abiotic stress-responsive genes. The overexpression of PheWRKY72-2 in Arabidopsis resulted in a decreased sensitivity to drought stress during early seedling growth. PheWRKY72-2 may enhance plant tolerance to stress by functioning as a positive regulator of stoma closure. Our study provides a theoretical foundation and some experimental evidence for further functional verification of the PheWRKY family of TFs.https://doi.org/10.1038/s41598-017-06701-2 |
| spellingShingle | Long Li Shaohua Mu Zhanchao Cheng Yuanwen Cheng Ying Zhang Ying Miao Chenglin Hou Xueping Li Jian Gao Characterization and expression analysis of the WRKY gene family in moso bamboo Scientific Reports |
| title | Characterization and expression analysis of the WRKY gene family in moso bamboo |
| title_full | Characterization and expression analysis of the WRKY gene family in moso bamboo |
| title_fullStr | Characterization and expression analysis of the WRKY gene family in moso bamboo |
| title_full_unstemmed | Characterization and expression analysis of the WRKY gene family in moso bamboo |
| title_short | Characterization and expression analysis of the WRKY gene family in moso bamboo |
| title_sort | characterization and expression analysis of the wrky gene family in moso bamboo |
| url | https://doi.org/10.1038/s41598-017-06701-2 |
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