Novel Lentivirus-Based Method for Rapid Selection of Inhibitory Nanobody against PRRSV
The emergence and re-emergence of porcine reproductive and respiratory syndrome virus (PRRSV) has resulted in huge economic losses for the swine industry. Current vaccines are of limited efficacy against endemic circulating PRRSV variants. New strategies against PRRSV infection are in urgent need. H...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/2/229 |
| _version_ | 1818558976328990720 |
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| author | Ze-Hui Liu Kai-Xia Lei Guang-Wei Han Hui-Ling Xu Fang He |
| author_facet | Ze-Hui Liu Kai-Xia Lei Guang-Wei Han Hui-Ling Xu Fang He |
| author_sort | Ze-Hui Liu |
| collection | DOAJ |
| description | The emergence and re-emergence of porcine reproductive and respiratory syndrome virus (PRRSV) has resulted in huge economic losses for the swine industry. Current vaccines are of limited efficacy against endemic circulating PRRSV variants. New strategies against PRRSV infection are in urgent need. Here, a nanobody library in Marc-145 cells is constructed for antiviral nanobodies. Nanobody encoding sequences from two non-immunized llamas were cloned to generate a pseudotyped lentiviral library. Several candidates were selected from survival cells post-PRRSV inoculation and further characterized. Nb9 was identified with strong antiviral activity. Moreover, Nb9 exerted antiviral activity via its interaction with PRRSV viral proteins, as revealed by immunofluorescence assay and Western blot. Taken together, the novel function-based screen of the lentivirus nanobody library, instead of the conventional affinity-based screen, offers an alternative strategy for antiviral reagents against PRRSV and other pathogens. |
| first_indexed | 2024-12-14T00:19:20Z |
| format | Article |
| id | doaj.art-8deeab02216a43719b12c274fabcc298 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-14T00:19:20Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-8deeab02216a43719b12c274fabcc2982022-12-21T23:25:18ZengMDPI AGViruses1999-49152020-02-0112222910.3390/v12020229v12020229Novel Lentivirus-Based Method for Rapid Selection of Inhibitory Nanobody against PRRSVZe-Hui Liu0Kai-Xia Lei1Guang-Wei Han2Hui-Ling Xu3Fang He4Institute of Preventive Veterinary Medicine, College of Animal Sciences of Zhejiang University, Hangzhou 310058, ChinaInstitute of Preventive Veterinary Medicine, College of Animal Sciences of Zhejiang University, Hangzhou 310058, ChinaInstitute of Preventive Veterinary Medicine, College of Animal Sciences of Zhejiang University, Hangzhou 310058, ChinaInstitute of Preventive Veterinary Medicine, College of Animal Sciences of Zhejiang University, Hangzhou 310058, ChinaInstitute of Preventive Veterinary Medicine, College of Animal Sciences of Zhejiang University, Hangzhou 310058, ChinaThe emergence and re-emergence of porcine reproductive and respiratory syndrome virus (PRRSV) has resulted in huge economic losses for the swine industry. Current vaccines are of limited efficacy against endemic circulating PRRSV variants. New strategies against PRRSV infection are in urgent need. Here, a nanobody library in Marc-145 cells is constructed for antiviral nanobodies. Nanobody encoding sequences from two non-immunized llamas were cloned to generate a pseudotyped lentiviral library. Several candidates were selected from survival cells post-PRRSV inoculation and further characterized. Nb9 was identified with strong antiviral activity. Moreover, Nb9 exerted antiviral activity via its interaction with PRRSV viral proteins, as revealed by immunofluorescence assay and Western blot. Taken together, the novel function-based screen of the lentivirus nanobody library, instead of the conventional affinity-based screen, offers an alternative strategy for antiviral reagents against PRRSV and other pathogens.https://www.mdpi.com/1999-4915/12/2/229prrsvantiviral strategylentivirus based nanobody librarynanobody selection |
| spellingShingle | Ze-Hui Liu Kai-Xia Lei Guang-Wei Han Hui-Ling Xu Fang He Novel Lentivirus-Based Method for Rapid Selection of Inhibitory Nanobody against PRRSV Viruses prrsv antiviral strategy lentivirus based nanobody library nanobody selection |
| title | Novel Lentivirus-Based Method for Rapid Selection of Inhibitory Nanobody against PRRSV |
| title_full | Novel Lentivirus-Based Method for Rapid Selection of Inhibitory Nanobody against PRRSV |
| title_fullStr | Novel Lentivirus-Based Method for Rapid Selection of Inhibitory Nanobody against PRRSV |
| title_full_unstemmed | Novel Lentivirus-Based Method for Rapid Selection of Inhibitory Nanobody against PRRSV |
| title_short | Novel Lentivirus-Based Method for Rapid Selection of Inhibitory Nanobody against PRRSV |
| title_sort | novel lentivirus based method for rapid selection of inhibitory nanobody against prrsv |
| topic | prrsv antiviral strategy lentivirus based nanobody library nanobody selection |
| url | https://www.mdpi.com/1999-4915/12/2/229 |
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