Interaction of Selected Commercial Antiseptics with Natural Products against Methicillin-Resistant <i>Staphylococcus aureus</i> Strain
The carriage of methicillin-resistant <i>S. aureus</i> (MRSA) strains may determine the subsequent occurrence of infection, which mainly affects patients after surgeries. Therefore, its eradication with the use of antibiotics or antiseptics is a crucial method of preventing staphylococca...
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MDPI AG
2024-03-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/14/5/2060 |
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| author | Monika Sienkiewicz Paulina Młodzińska Anna Kilanowicz Ewa Dudzińska Paweł Kwiatkowski |
| author_facet | Monika Sienkiewicz Paulina Młodzińska Anna Kilanowicz Ewa Dudzińska Paweł Kwiatkowski |
| author_sort | Monika Sienkiewicz |
| collection | DOAJ |
| description | The carriage of methicillin-resistant <i>S. aureus</i> (MRSA) strains may determine the subsequent occurrence of infection, which mainly affects patients after surgeries. Therefore, its eradication with the use of antibiotics or antiseptics is a crucial method of preventing staphylococcal self-infections. The observed increase in bacterial resistance to known antibacterial substances poses a huge challenge to scientists. The aim of this study was to analyze the interaction of selected commercial antiseptics (Braunol, CITROclorex 2%, Microdacyn 60 Wound Care, Octenisept and Prontoderm Solution) with natural products (carvacrol, β-citronellol, geraniol, eugenol, farnesol, (-)-menthone, eucalyptol, limonene, linalyl acetate and <i>trans</i>-anethole) against the MRSA standard strain. The antistaphylococcal activity of commercial antiseptics in combination with natural products against MRSA was determined using the checkerboard method. The obtained results showed that most combinations decreased the MIC level of the commercial antiseptic and natural compounds. A statistically significant decrease in the MIC value of the antiseptic and natural constituent was observed for the Braunol–β-citronellol combination. Such a significant decrease in the MIC value of the natural compound against the analyzed strain was also detected for the Octenisept–β-citronellol and Prontoderm Solution–β-citronellol combinations. The interaction analysis showed that out of all 20 combinations of individual antiseptics with substances of natural origin, two combinations showed a synergistic effect (Braunol–β-citronellol, Braunol–carvacrol), and three combinations showed an additive effect (Braunol–eugenol, Braunol–geraniol, Prontoderm Solution–β-citronellol). The use of Braunol in combination with β-citronellol or carvacrol may become an alternative method of eradicating MRSA strains. However, further studies are needed to determine the toxicity of the analyzed combinations. |
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| language | English |
| last_indexed | 2024-04-25T00:34:30Z |
| publishDate | 2024-03-01 |
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| spelling | doaj.art-8df387c25577460b9a4c6cdc15de77332024-03-12T16:39:58ZengMDPI AGApplied Sciences2076-34172024-03-01145206010.3390/app14052060Interaction of Selected Commercial Antiseptics with Natural Products against Methicillin-Resistant <i>Staphylococcus aureus</i> StrainMonika Sienkiewicz0Paulina Młodzińska1Anna Kilanowicz2Ewa Dudzińska3Paweł Kwiatkowski4Department of Pharmaceutical Microbiology and Microbiological Diagnostic, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, PolandDepartment of Diagnostic Immunology, Pomeranian Medical University in Szczecin, al. Powstancow Wielkopolskich 72, 70-111 Szczecin, PolandDepartment of Toxicology, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, PolandDepartment of Dietetics and Nutrition Education, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Diagnostic Immunology, Pomeranian Medical University in Szczecin, al. Powstancow Wielkopolskich 72, 70-111 Szczecin, PolandThe carriage of methicillin-resistant <i>S. aureus</i> (MRSA) strains may determine the subsequent occurrence of infection, which mainly affects patients after surgeries. Therefore, its eradication with the use of antibiotics or antiseptics is a crucial method of preventing staphylococcal self-infections. The observed increase in bacterial resistance to known antibacterial substances poses a huge challenge to scientists. The aim of this study was to analyze the interaction of selected commercial antiseptics (Braunol, CITROclorex 2%, Microdacyn 60 Wound Care, Octenisept and Prontoderm Solution) with natural products (carvacrol, β-citronellol, geraniol, eugenol, farnesol, (-)-menthone, eucalyptol, limonene, linalyl acetate and <i>trans</i>-anethole) against the MRSA standard strain. The antistaphylococcal activity of commercial antiseptics in combination with natural products against MRSA was determined using the checkerboard method. The obtained results showed that most combinations decreased the MIC level of the commercial antiseptic and natural compounds. A statistically significant decrease in the MIC value of the antiseptic and natural constituent was observed for the Braunol–β-citronellol combination. Such a significant decrease in the MIC value of the natural compound against the analyzed strain was also detected for the Octenisept–β-citronellol and Prontoderm Solution–β-citronellol combinations. The interaction analysis showed that out of all 20 combinations of individual antiseptics with substances of natural origin, two combinations showed a synergistic effect (Braunol–β-citronellol, Braunol–carvacrol), and three combinations showed an additive effect (Braunol–eugenol, Braunol–geraniol, Prontoderm Solution–β-citronellol). The use of Braunol in combination with β-citronellol or carvacrol may become an alternative method of eradicating MRSA strains. However, further studies are needed to determine the toxicity of the analyzed combinations.https://www.mdpi.com/2076-3417/14/5/2060MRSAnatural productsessential oil compoundsantisepticscheckerboard methodsynergistic activity |
| spellingShingle | Monika Sienkiewicz Paulina Młodzińska Anna Kilanowicz Ewa Dudzińska Paweł Kwiatkowski Interaction of Selected Commercial Antiseptics with Natural Products against Methicillin-Resistant <i>Staphylococcus aureus</i> Strain Applied Sciences MRSA natural products essential oil compounds antiseptics checkerboard method synergistic activity |
| title | Interaction of Selected Commercial Antiseptics with Natural Products against Methicillin-Resistant <i>Staphylococcus aureus</i> Strain |
| title_full | Interaction of Selected Commercial Antiseptics with Natural Products against Methicillin-Resistant <i>Staphylococcus aureus</i> Strain |
| title_fullStr | Interaction of Selected Commercial Antiseptics with Natural Products against Methicillin-Resistant <i>Staphylococcus aureus</i> Strain |
| title_full_unstemmed | Interaction of Selected Commercial Antiseptics with Natural Products against Methicillin-Resistant <i>Staphylococcus aureus</i> Strain |
| title_short | Interaction of Selected Commercial Antiseptics with Natural Products against Methicillin-Resistant <i>Staphylococcus aureus</i> Strain |
| title_sort | interaction of selected commercial antiseptics with natural products against methicillin resistant i staphylococcus aureus i strain |
| topic | MRSA natural products essential oil compounds antiseptics checkerboard method synergistic activity |
| url | https://www.mdpi.com/2076-3417/14/5/2060 |
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