Goji-Berry-Mediated Green Synthesis of Gold Nanoparticles and Their Promising Effect on Reducing Oxidative Stress and Inflammation in Experimental Hyperglycemia
The present report focuses on a rapid and convenient method applicable in the green synthesis of gold nanoparticles (AuNPs) using goji berry (<i>Lycium barbarum</i>—LB) extracts rich in antioxidant compounds, as well as on the structural analysis and evaluation of the induced antioxidant...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/8/1489 |
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| author | Luminita David Valentina Morosan Bianca Moldovan Gabriela Adriana Filip Ioana Baldea |
| author_facet | Luminita David Valentina Morosan Bianca Moldovan Gabriela Adriana Filip Ioana Baldea |
| author_sort | Luminita David |
| collection | DOAJ |
| description | The present report focuses on a rapid and convenient method applicable in the green synthesis of gold nanoparticles (AuNPs) using goji berry (<i>Lycium barbarum</i>—LB) extracts rich in antioxidant compounds, as well as on the structural analysis and evaluation of the induced antioxidant protection and anti-inflammatory effects of the synthesized gold nanoparticles upon endothelial cells (HUVECs) exposed to hyperglycemia. The synthesized AuNPs were characterized using ultraviolet–visible (UV-Vis) spectroscopy and transmission electron microscopy (TEM), whereas the presence of bioactive compounds from the <i>L. barbarum</i> fruit extract on the surface of the nanoparticles was confirmed using Fourier transform infrared spectroscopy (FTIR). The antioxidant activity of the biosynthesized gold nanoparticles was evaluated on the HUVEC cell line. The results reveal that AuNPs with a predominantly spherical shape and an average size of 30 nm were obtained. The UV-Vis spectrum showed a characteristic absorption band at λ<sub>max</sub> = 536 nm of AuNPs. FTIR analysis revealed the presence of phenolic acids, flavonoids and carotenoids acting as capping and stabilizing agents of AuNPs. Both the <i>L. barbarum</i> extract and AuNPs were well tolerated by HUVECs, increased the antioxidant defense and decreased the production of inflammatory cytokines induced via hyperglycemia-mediated oxidative damage. |
| first_indexed | 2024-03-11T00:10:42Z |
| format | Article |
| id | doaj.art-8df603fea77841f1940fdc4c81bd4f45 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T00:10:42Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-8df603fea77841f1940fdc4c81bd4f452023-11-19T00:00:34ZengMDPI AGAntioxidants2076-39212023-07-01128148910.3390/antiox12081489Goji-Berry-Mediated Green Synthesis of Gold Nanoparticles and Their Promising Effect on Reducing Oxidative Stress and Inflammation in Experimental HyperglycemiaLuminita David0Valentina Morosan1Bianca Moldovan2Gabriela Adriana Filip3Ioana Baldea4Faculty of Chemistry and Chemical Engineering, “Babeş-Bolyai” University, 11 Arany Janos Street, 400028 Cluj-Napoca, RomaniaFaculty of Chemistry and Chemical Engineering, “Babeş-Bolyai” University, 11 Arany Janos Street, 400028 Cluj-Napoca, RomaniaFaculty of Chemistry and Chemical Engineering, “Babeş-Bolyai” University, 11 Arany Janos Street, 400028 Cluj-Napoca, RomaniaDepartment of Physiology, “Iuliu Haţieganu” University of Medicine and Pharmacy, 1-3 Clinicilor Street, 400006 Cluj-Napoca, RomaniaDepartment of Physiology, “Iuliu Haţieganu” University of Medicine and Pharmacy, 1-3 Clinicilor Street, 400006 Cluj-Napoca, RomaniaThe present report focuses on a rapid and convenient method applicable in the green synthesis of gold nanoparticles (AuNPs) using goji berry (<i>Lycium barbarum</i>—LB) extracts rich in antioxidant compounds, as well as on the structural analysis and evaluation of the induced antioxidant protection and anti-inflammatory effects of the synthesized gold nanoparticles upon endothelial cells (HUVECs) exposed to hyperglycemia. The synthesized AuNPs were characterized using ultraviolet–visible (UV-Vis) spectroscopy and transmission electron microscopy (TEM), whereas the presence of bioactive compounds from the <i>L. barbarum</i> fruit extract on the surface of the nanoparticles was confirmed using Fourier transform infrared spectroscopy (FTIR). The antioxidant activity of the biosynthesized gold nanoparticles was evaluated on the HUVEC cell line. The results reveal that AuNPs with a predominantly spherical shape and an average size of 30 nm were obtained. The UV-Vis spectrum showed a characteristic absorption band at λ<sub>max</sub> = 536 nm of AuNPs. FTIR analysis revealed the presence of phenolic acids, flavonoids and carotenoids acting as capping and stabilizing agents of AuNPs. Both the <i>L. barbarum</i> extract and AuNPs were well tolerated by HUVECs, increased the antioxidant defense and decreased the production of inflammatory cytokines induced via hyperglycemia-mediated oxidative damage.https://www.mdpi.com/2076-3921/12/8/1489green synthesisgoji berriesgold nanoparticlesantioxidant activity |
| spellingShingle | Luminita David Valentina Morosan Bianca Moldovan Gabriela Adriana Filip Ioana Baldea Goji-Berry-Mediated Green Synthesis of Gold Nanoparticles and Their Promising Effect on Reducing Oxidative Stress and Inflammation in Experimental Hyperglycemia Antioxidants green synthesis goji berries gold nanoparticles antioxidant activity |
| title | Goji-Berry-Mediated Green Synthesis of Gold Nanoparticles and Their Promising Effect on Reducing Oxidative Stress and Inflammation in Experimental Hyperglycemia |
| title_full | Goji-Berry-Mediated Green Synthesis of Gold Nanoparticles and Their Promising Effect on Reducing Oxidative Stress and Inflammation in Experimental Hyperglycemia |
| title_fullStr | Goji-Berry-Mediated Green Synthesis of Gold Nanoparticles and Their Promising Effect on Reducing Oxidative Stress and Inflammation in Experimental Hyperglycemia |
| title_full_unstemmed | Goji-Berry-Mediated Green Synthesis of Gold Nanoparticles and Their Promising Effect on Reducing Oxidative Stress and Inflammation in Experimental Hyperglycemia |
| title_short | Goji-Berry-Mediated Green Synthesis of Gold Nanoparticles and Their Promising Effect on Reducing Oxidative Stress and Inflammation in Experimental Hyperglycemia |
| title_sort | goji berry mediated green synthesis of gold nanoparticles and their promising effect on reducing oxidative stress and inflammation in experimental hyperglycemia |
| topic | green synthesis goji berries gold nanoparticles antioxidant activity |
| url | https://www.mdpi.com/2076-3921/12/8/1489 |
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