Differential Transmission of Old and New World Begomoviruses by Middle East-Asia Minor 1 (MEAM1) and Mediterranean (MED) Cryptic Species of <em>Bemisia tabaci</em>
Middle East-Asia Minor 1 (MEAM1) and Mediterranean (MED) are two of the most invasive members of the sweetpotato whitefly, <i>Bemisia tabaci,</i> cryptic species complexes and are efficient vectors of begomoviruses. <i>Bemisia tabaci</i> MEAM1 is the predominant vector of beg...
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MDPI AG
2022-05-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/5/1104 |
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| author | Saurabh Gautam Habibu Mugerwa James W. Buck Bhabesh Dutta Tim Coolong Scott Adkins Rajagopalbabu Srinivasan |
| author_facet | Saurabh Gautam Habibu Mugerwa James W. Buck Bhabesh Dutta Tim Coolong Scott Adkins Rajagopalbabu Srinivasan |
| author_sort | Saurabh Gautam |
| collection | DOAJ |
| description | Middle East-Asia Minor 1 (MEAM1) and Mediterranean (MED) are two of the most invasive members of the sweetpotato whitefly, <i>Bemisia tabaci,</i> cryptic species complexes and are efficient vectors of begomoviruses. <i>Bemisia tabaci</i> MEAM1 is the predominant vector of begomoviruses in open-field vegetable crops in the southeastern United States. However, recently <i>B. tabaci</i> MED also has been detected in the landscape outside of greenhouses in Florida and Georgia. This study compared the transmission efficiency of one Old-World (OW) and two New-World (NW) begomoviruses prevalent in the southeastern United States, viz., tomato yellow leaf curl virus (TYLCV), cucurbit leaf crumple virus (CuLCrV), and sida golden mosaic virus (SiGMV) between <i>B. tabaci</i> MEAM1 and <i>B. tabaci</i> MED. <i>Bemisia tabaci</i> MEAM1 efficiently transmitted TYLCV, CuLCrV, or SiGMV, whereas <i>B. tabaci</i> MED only transmitted TYLCV. Percent acquisition and retention of OW TYLCV following a 72 h acquisition access period was significantly higher for <i>B. tabaci</i> MED than <i>B. tabaci</i> MEAM1. In contrast, <i>B. tabaci</i> MEAM1 acquired and retained significantly more NW bipartite begomoviruses, CuLCrV or SiGMV, than <i>B. tabaci</i> MED. Quantitative analysis (qPCR) of virus DNA in whitefly internal tissues revealed reduced accumulation of CuLCrV or SiGMV in <i>B. tabaci</i> MED than in <i>B. tabaci</i> MEAM1. Fluorescent in situ hybridization (FISH) showed localization of CuLCrV or SiGMV in the midgut of <i>B. tabaci</i> MED and <i>B. tabaci</i> MEAM1. However, localization of CuLCrV or SiGMV was only observed in the primary salivary glands of <i>B. tabaci</i> MEAM1 and not <i>B. tabaci</i> MED. TYLCV localization was observed in all internal tissues of <i>B. tabaci</i> MEAM1 and <i>B. tabaci</i> MED. Overall, results demonstrate that both <i>B. tabaci</i> MEAM1 and <i>B. tabaci</i> MED are efficient vectors of OW TYLCV. However, for the NW begomoviruses, CuLCrV and SiGMV, <i>B. tabaci</i> MEAM1 seems to a better vector. |
| first_indexed | 2024-03-10T01:35:41Z |
| format | Article |
| id | doaj.art-8df61a629cf046cbab8217795827d759 |
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| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T01:35:41Z |
| publishDate | 2022-05-01 |
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| series | Viruses |
| spelling | doaj.art-8df61a629cf046cbab8217795827d7592023-11-23T13:33:22ZengMDPI AGViruses1999-49152022-05-01145110410.3390/v14051104Differential Transmission of Old and New World Begomoviruses by Middle East-Asia Minor 1 (MEAM1) and Mediterranean (MED) Cryptic Species of <em>Bemisia tabaci</em>Saurabh Gautam0Habibu Mugerwa1James W. Buck2Bhabesh Dutta3Tim Coolong4Scott Adkins5Rajagopalbabu Srinivasan6Department of Entomology, University of Georgia, 1109 Experiment Street, Griffin, GA 30223, USADepartment of Entomology, University of Georgia, 1109 Experiment Street, Griffin, GA 30223, USADepartment of Plant Pathology, University of Georgia, 1109 Experiment Street, Griffin, GA 30223, USADepartment of Plant Pathology, University of Georgia, 3250 Rainwater Road, Tifton, GA 31793, USADepartment of Horticulture, University of Georgia, 3250 Rainwater Road, Tifton, GA 31793, USAUnited States Department of Agriculture-Agricultural Research Service, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945, USADepartment of Entomology, University of Georgia, 1109 Experiment Street, Griffin, GA 30223, USAMiddle East-Asia Minor 1 (MEAM1) and Mediterranean (MED) are two of the most invasive members of the sweetpotato whitefly, <i>Bemisia tabaci,</i> cryptic species complexes and are efficient vectors of begomoviruses. <i>Bemisia tabaci</i> MEAM1 is the predominant vector of begomoviruses in open-field vegetable crops in the southeastern United States. However, recently <i>B. tabaci</i> MED also has been detected in the landscape outside of greenhouses in Florida and Georgia. This study compared the transmission efficiency of one Old-World (OW) and two New-World (NW) begomoviruses prevalent in the southeastern United States, viz., tomato yellow leaf curl virus (TYLCV), cucurbit leaf crumple virus (CuLCrV), and sida golden mosaic virus (SiGMV) between <i>B. tabaci</i> MEAM1 and <i>B. tabaci</i> MED. <i>Bemisia tabaci</i> MEAM1 efficiently transmitted TYLCV, CuLCrV, or SiGMV, whereas <i>B. tabaci</i> MED only transmitted TYLCV. Percent acquisition and retention of OW TYLCV following a 72 h acquisition access period was significantly higher for <i>B. tabaci</i> MED than <i>B. tabaci</i> MEAM1. In contrast, <i>B. tabaci</i> MEAM1 acquired and retained significantly more NW bipartite begomoviruses, CuLCrV or SiGMV, than <i>B. tabaci</i> MED. Quantitative analysis (qPCR) of virus DNA in whitefly internal tissues revealed reduced accumulation of CuLCrV or SiGMV in <i>B. tabaci</i> MED than in <i>B. tabaci</i> MEAM1. Fluorescent in situ hybridization (FISH) showed localization of CuLCrV or SiGMV in the midgut of <i>B. tabaci</i> MED and <i>B. tabaci</i> MEAM1. However, localization of CuLCrV or SiGMV was only observed in the primary salivary glands of <i>B. tabaci</i> MEAM1 and not <i>B. tabaci</i> MED. TYLCV localization was observed in all internal tissues of <i>B. tabaci</i> MEAM1 and <i>B. tabaci</i> MED. Overall, results demonstrate that both <i>B. tabaci</i> MEAM1 and <i>B. tabaci</i> MED are efficient vectors of OW TYLCV. However, for the NW begomoviruses, CuLCrV and SiGMV, <i>B. tabaci</i> MEAM1 seems to a better vector.https://www.mdpi.com/1999-4915/14/5/1104cucurbit leaf crumple virusmidgutprimary salivary glandssida golden mosaic virustomato yellow leaf curl virus |
| spellingShingle | Saurabh Gautam Habibu Mugerwa James W. Buck Bhabesh Dutta Tim Coolong Scott Adkins Rajagopalbabu Srinivasan Differential Transmission of Old and New World Begomoviruses by Middle East-Asia Minor 1 (MEAM1) and Mediterranean (MED) Cryptic Species of <em>Bemisia tabaci</em> Viruses cucurbit leaf crumple virus midgut primary salivary glands sida golden mosaic virus tomato yellow leaf curl virus |
| title | Differential Transmission of Old and New World Begomoviruses by Middle East-Asia Minor 1 (MEAM1) and Mediterranean (MED) Cryptic Species of <em>Bemisia tabaci</em> |
| title_full | Differential Transmission of Old and New World Begomoviruses by Middle East-Asia Minor 1 (MEAM1) and Mediterranean (MED) Cryptic Species of <em>Bemisia tabaci</em> |
| title_fullStr | Differential Transmission of Old and New World Begomoviruses by Middle East-Asia Minor 1 (MEAM1) and Mediterranean (MED) Cryptic Species of <em>Bemisia tabaci</em> |
| title_full_unstemmed | Differential Transmission of Old and New World Begomoviruses by Middle East-Asia Minor 1 (MEAM1) and Mediterranean (MED) Cryptic Species of <em>Bemisia tabaci</em> |
| title_short | Differential Transmission of Old and New World Begomoviruses by Middle East-Asia Minor 1 (MEAM1) and Mediterranean (MED) Cryptic Species of <em>Bemisia tabaci</em> |
| title_sort | differential transmission of old and new world begomoviruses by middle east asia minor 1 meam1 and mediterranean med cryptic species of em bemisia tabaci em |
| topic | cucurbit leaf crumple virus midgut primary salivary glands sida golden mosaic virus tomato yellow leaf curl virus |
| url | https://www.mdpi.com/1999-4915/14/5/1104 |
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