LA-MRSA CC398 in Dairy Cattle and Veal Calf Farms Indicates Spillover From Pig Production
The possible spillover from pigs into other production animals incites concern for unresolved reservoirs of human exposure. The present investigation was therefore initiated, to elucidate if Danish veal and dairy farms constitute a reservoir of livestock-associated methicillin-resistant Staphylococc...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02733/full |
| _version_ | 1830485016061149184 |
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| author | Julie E. Hansen Troels Ronco Marc Stegger Raphael N. Sieber Mette E. Fertner Henrik L. Martin Michael Farre Nils Toft Anders R. Larsen Karl Pedersen |
| author_facet | Julie E. Hansen Troels Ronco Marc Stegger Raphael N. Sieber Mette E. Fertner Henrik L. Martin Michael Farre Nils Toft Anders R. Larsen Karl Pedersen |
| author_sort | Julie E. Hansen |
| collection | DOAJ |
| description | The possible spillover from pigs into other production animals incites concern for unresolved reservoirs of human exposure. The present investigation was therefore initiated, to elucidate if Danish veal and dairy farms constitute a reservoir of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) CC398 and to potentially identify the source of introduction. We collected nasal swab samples from 17 Danish veal farms, 2 slaughterhouses, and received bulk tank milk samples from 286 dairy farms. All samples were analyzed by culturing and screening on MRSA selective plates and presumed MRSA was verified by MALDI-TOF and PCR. MRSA isolates were subjected to spa typing and whole-genome sequencing. LA-MRSA was found on two veal farms in one and three calves, respectively, with subsequent follow-up samples found negative. Eight of 286 dairy farms (2.8%) were found LA-MRSA positive and follow-up samples, from five farms showed intermittent detection of LA-MRSA. The spa types, t034 and t011, were the most common while a single isolate from a dairy farm belonged to spa type t843 associated to mecC-MRSA CC130 and is the first report of mecC-MRSA in the Danish dairy production. A phylogenetic analysis showed that some of the isolates grouped within or close to the dominant Danish pig clusters, suggesting spillover into cattle farms. Other isolates clustered outside the dominant pig clusters suggesting that other routes of introduction cannot be excluded. Results of the investigation indicated a contamination of veal farms while some dairy farms seemed to be a permanent reservoir. Thus, Danish cattle represent a low prevalence reservoir of LA-MRSA CC398, which at present, is not of major human health concern. |
| first_indexed | 2024-12-21T18:18:39Z |
| format | Article |
| id | doaj.art-8df7122cfb644d8da1babffa70f74bc9 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-21T18:18:39Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-8df7122cfb644d8da1babffa70f74bc92022-12-21T18:54:36ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-11-011010.3389/fmicb.2019.02733491642LA-MRSA CC398 in Dairy Cattle and Veal Calf Farms Indicates Spillover From Pig ProductionJulie E. Hansen0Troels Ronco1Marc Stegger2Raphael N. Sieber3Mette E. Fertner4Henrik L. Martin5Michael Farre6Nils Toft7Anders R. Larsen8Karl Pedersen9National Veterinary Institute, Technical University of Denmark, Lyngby, DenmarkNational Veterinary Institute, Technical University of Denmark, Lyngby, DenmarkDepartment of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, DenmarkDepartment of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, DenmarkNational Veterinary Institute, Technical University of Denmark, Lyngby, DenmarkSEGES P/S, Livestock Innovation, Aarhus, DenmarkSEGES P/S, Livestock Innovation, Aarhus, DenmarkNational Veterinary Institute, Technical University of Denmark, Lyngby, DenmarkDepartment of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, DenmarkNational Veterinary Institute, Uppsala, SwedenThe possible spillover from pigs into other production animals incites concern for unresolved reservoirs of human exposure. The present investigation was therefore initiated, to elucidate if Danish veal and dairy farms constitute a reservoir of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) CC398 and to potentially identify the source of introduction. We collected nasal swab samples from 17 Danish veal farms, 2 slaughterhouses, and received bulk tank milk samples from 286 dairy farms. All samples were analyzed by culturing and screening on MRSA selective plates and presumed MRSA was verified by MALDI-TOF and PCR. MRSA isolates were subjected to spa typing and whole-genome sequencing. LA-MRSA was found on two veal farms in one and three calves, respectively, with subsequent follow-up samples found negative. Eight of 286 dairy farms (2.8%) were found LA-MRSA positive and follow-up samples, from five farms showed intermittent detection of LA-MRSA. The spa types, t034 and t011, were the most common while a single isolate from a dairy farm belonged to spa type t843 associated to mecC-MRSA CC130 and is the first report of mecC-MRSA in the Danish dairy production. A phylogenetic analysis showed that some of the isolates grouped within or close to the dominant Danish pig clusters, suggesting spillover into cattle farms. Other isolates clustered outside the dominant pig clusters suggesting that other routes of introduction cannot be excluded. Results of the investigation indicated a contamination of veal farms while some dairy farms seemed to be a permanent reservoir. Thus, Danish cattle represent a low prevalence reservoir of LA-MRSA CC398, which at present, is not of major human health concern.https://www.frontiersin.org/article/10.3389/fmicb.2019.02733/fullbulk tank milkveal calvescattlemethicillin resistant Staphylococcus aureusCC398mecC |
| spellingShingle | Julie E. Hansen Troels Ronco Marc Stegger Raphael N. Sieber Mette E. Fertner Henrik L. Martin Michael Farre Nils Toft Anders R. Larsen Karl Pedersen LA-MRSA CC398 in Dairy Cattle and Veal Calf Farms Indicates Spillover From Pig Production Frontiers in Microbiology bulk tank milk veal calves cattle methicillin resistant Staphylococcus aureus CC398 mecC |
| title | LA-MRSA CC398 in Dairy Cattle and Veal Calf Farms Indicates Spillover From Pig Production |
| title_full | LA-MRSA CC398 in Dairy Cattle and Veal Calf Farms Indicates Spillover From Pig Production |
| title_fullStr | LA-MRSA CC398 in Dairy Cattle and Veal Calf Farms Indicates Spillover From Pig Production |
| title_full_unstemmed | LA-MRSA CC398 in Dairy Cattle and Veal Calf Farms Indicates Spillover From Pig Production |
| title_short | LA-MRSA CC398 in Dairy Cattle and Veal Calf Farms Indicates Spillover From Pig Production |
| title_sort | la mrsa cc398 in dairy cattle and veal calf farms indicates spillover from pig production |
| topic | bulk tank milk veal calves cattle methicillin resistant Staphylococcus aureus CC398 mecC |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02733/full |
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