A New Sturgeon Herpesvirus from Juvenile Lake Sturgeon <i>Acipenser fulvescens</i> Displaying Epithelial Skin Lesions
Herpesvirus infections of sturgeon pose a potential threat to sturgeon culture efforts worldwide. A new epitheliotropic herpesvirus named Acipenser herpesvirus 3 (AciHV-3) was detected in hatchery-reared Lake Sturgeon <i>Acipenser fulvescens</i> displaying skin lesions in central Canada....
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MDPI AG
2023-08-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/9/1115 |
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| author | Sharon Clouthier Marek Tomczyk Tamara Schroeder Cheryl Klassen André Dufresne Eveline Emmenegger Thomas Nalpathamkalam Zhuozhi Wang Bhooma Thiruvahindrapuram |
| author_facet | Sharon Clouthier Marek Tomczyk Tamara Schroeder Cheryl Klassen André Dufresne Eveline Emmenegger Thomas Nalpathamkalam Zhuozhi Wang Bhooma Thiruvahindrapuram |
| author_sort | Sharon Clouthier |
| collection | DOAJ |
| description | Herpesvirus infections of sturgeon pose a potential threat to sturgeon culture efforts worldwide. A new epitheliotropic herpesvirus named Acipenser herpesvirus 3 (AciHV-3) was detected in hatchery-reared Lake Sturgeon <i>Acipenser fulvescens</i> displaying skin lesions in central Canada. The growths were discovered in the fall, reached average prevalence levels of 0.2–40% and eventually regressed. No unusual mortality was observed. The cellular changes within the lesions included epithelial hyperplasia and were reminiscent of other herpesvirus infections. The virus was not evident in lesions examined by electron microscopy. Skin tissue homogenates from symptomatic sturgeon produced atypical cytopathic effects on a primary Lake Sturgeon cell line, and next-generation sequence analysis of the DNA samples revealed the presence of an alloherpesvirus. A new genotyping PCR assay targeting the major capsid protein sequence detected AciHV-3 in symptomatic Lake Sturgeon as well as other apparently healthy sturgeon species. Bayesian inference of phylogeny reconstructed with a concatenation of five alloherpesvirus core proteins revealed a new <i>Alloherpesviridae</i> lineage isomorphic with a new genus. The presence of AciHV-3 homologs in cell lines and sturgeon sequence datasets, low sequence divergence among these homologs and branching patterns within the genotyping phylogeny provide preliminary evidence of an endogenous virus lifestyle established in an ancestral sturgeon. |
| first_indexed | 2024-03-10T22:18:31Z |
| format | Article |
| id | doaj.art-8df8867407ca427584c9998607d4bf66 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T22:18:31Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-8df8867407ca427584c9998607d4bf662023-11-19T12:22:15ZengMDPI AGPathogens2076-08172023-08-01129111510.3390/pathogens12091115A New Sturgeon Herpesvirus from Juvenile Lake Sturgeon <i>Acipenser fulvescens</i> Displaying Epithelial Skin LesionsSharon Clouthier0Marek Tomczyk1Tamara Schroeder2Cheryl Klassen3André Dufresne4Eveline Emmenegger5Thomas Nalpathamkalam6Zhuozhi Wang7Bhooma Thiruvahindrapuram8Freshwater Institute, Department of Fisheries and Oceans, 501 University Crescent, Winnipeg, MB R3T 2N6, CanadaManitoba Agriculture & Resource Development Veterinary Diagnostic Services, 545 University Crescent, Winnipeg, MB R3T 5S6, CanadaFreshwater Institute, Department of Fisheries and Oceans, 501 University Crescent, Winnipeg, MB R3T 2N6, CanadaManitoba Hydro, 360 Portage Ave, Winnipeg, MB R3C 0G8, CanadaNational Centre for Foreign Animal Disease, Canadian Food Inspection Agency, 1015 Arlington Street, Winnipeg, MB R3E 3M4, CanadaWestern Fisheries Research Center, U.S. Geological Survey, 6505 NE 65th Street, Seattle, WA 98115, USAThe Centre for Applied Genomics, The Hospital for Sick Children, 686 Bay Street, Toronto, ON M5G 0A4, CanadaThe Centre for Applied Genomics, The Hospital for Sick Children, 686 Bay Street, Toronto, ON M5G 0A4, CanadaThe Centre for Applied Genomics, The Hospital for Sick Children, 686 Bay Street, Toronto, ON M5G 0A4, CanadaHerpesvirus infections of sturgeon pose a potential threat to sturgeon culture efforts worldwide. A new epitheliotropic herpesvirus named Acipenser herpesvirus 3 (AciHV-3) was detected in hatchery-reared Lake Sturgeon <i>Acipenser fulvescens</i> displaying skin lesions in central Canada. The growths were discovered in the fall, reached average prevalence levels of 0.2–40% and eventually regressed. No unusual mortality was observed. The cellular changes within the lesions included epithelial hyperplasia and were reminiscent of other herpesvirus infections. The virus was not evident in lesions examined by electron microscopy. Skin tissue homogenates from symptomatic sturgeon produced atypical cytopathic effects on a primary Lake Sturgeon cell line, and next-generation sequence analysis of the DNA samples revealed the presence of an alloherpesvirus. A new genotyping PCR assay targeting the major capsid protein sequence detected AciHV-3 in symptomatic Lake Sturgeon as well as other apparently healthy sturgeon species. Bayesian inference of phylogeny reconstructed with a concatenation of five alloherpesvirus core proteins revealed a new <i>Alloherpesviridae</i> lineage isomorphic with a new genus. The presence of AciHV-3 homologs in cell lines and sturgeon sequence datasets, low sequence divergence among these homologs and branching patterns within the genotyping phylogeny provide preliminary evidence of an endogenous virus lifestyle established in an ancestral sturgeon.https://www.mdpi.com/2076-0817/12/9/1115Acipenser herpesvirusAciHV-3<i>Alloherpesviridae</i>epidermal hyperplasianext-generation sequencingNGS |
| spellingShingle | Sharon Clouthier Marek Tomczyk Tamara Schroeder Cheryl Klassen André Dufresne Eveline Emmenegger Thomas Nalpathamkalam Zhuozhi Wang Bhooma Thiruvahindrapuram A New Sturgeon Herpesvirus from Juvenile Lake Sturgeon <i>Acipenser fulvescens</i> Displaying Epithelial Skin Lesions Pathogens Acipenser herpesvirus AciHV-3 <i>Alloherpesviridae</i> epidermal hyperplasia next-generation sequencing NGS |
| title | A New Sturgeon Herpesvirus from Juvenile Lake Sturgeon <i>Acipenser fulvescens</i> Displaying Epithelial Skin Lesions |
| title_full | A New Sturgeon Herpesvirus from Juvenile Lake Sturgeon <i>Acipenser fulvescens</i> Displaying Epithelial Skin Lesions |
| title_fullStr | A New Sturgeon Herpesvirus from Juvenile Lake Sturgeon <i>Acipenser fulvescens</i> Displaying Epithelial Skin Lesions |
| title_full_unstemmed | A New Sturgeon Herpesvirus from Juvenile Lake Sturgeon <i>Acipenser fulvescens</i> Displaying Epithelial Skin Lesions |
| title_short | A New Sturgeon Herpesvirus from Juvenile Lake Sturgeon <i>Acipenser fulvescens</i> Displaying Epithelial Skin Lesions |
| title_sort | new sturgeon herpesvirus from juvenile lake sturgeon i acipenser fulvescens i displaying epithelial skin lesions |
| topic | Acipenser herpesvirus AciHV-3 <i>Alloherpesviridae</i> epidermal hyperplasia next-generation sequencing NGS |
| url | https://www.mdpi.com/2076-0817/12/9/1115 |
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