Antioxidant Activity of Purified Active Peptide Derived from Spirulina platensis Enzymatic Hydrolysates
The aim of this study is to isolate the antioxidative peptide from Spirulina platensis. Peptide was obtained by proteolytic digestion, ultrafiltration, fractionation by RP-HPLC, identified by LC-MS/MS—MASCOT Distiller and measured its antioxidant activity by DPPH (2.2-Diphenyl-1-picrylhydrazyl) assa...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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University of Brawijaya
2017-08-01
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| Series: | Research Journal of Life Science |
| Subjects: | |
| Online Access: | http://rjls.ub.ac.id/index.php/rjls/article/view/177 |
| _version_ | 1818390805549678592 |
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| author | Nur Maulida Safitri Endang Yuli Herawati Jue Liang Hsu |
| author_facet | Nur Maulida Safitri Endang Yuli Herawati Jue Liang Hsu |
| author_sort | Nur Maulida Safitri |
| collection | DOAJ |
| description | The aim of this study is to isolate the antioxidative peptide from Spirulina platensis. Peptide was obtained by proteolytic digestion, ultrafiltration, fractionation by RP-HPLC, identified by LC-MS/MS—MASCOT Distiller and measured its antioxidant activity by DPPH (2.2-Diphenyl-1-picrylhydrazyl) assay. Results showed that thermolysin was the most effective enzyme to digest this algae. The active peptide Phe-Ser-Glu-Ser-Ser-Ala-Pro-Glu-Gln-His-Tyr (m/z 1281.51) was identified and synthetized, which exhibited 45.98 ± 1.7% at concentration 128.15 µg/mL. Therefore, S. platensis is indicated as a potential therapeutic source for combating oxidative stress. |
| first_indexed | 2024-12-14T05:03:28Z |
| format | Article |
| id | doaj.art-8df9737bd25f4adc9f400cca0d1832d3 |
| institution | Directory Open Access Journal |
| issn | 2355-9926 |
| language | English |
| last_indexed | 2024-12-14T05:03:28Z |
| publishDate | 2017-08-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Research Journal of Life Science |
| spelling | doaj.art-8df9737bd25f4adc9f400cca0d1832d32022-12-21T23:16:10ZengUniversity of BrawijayaResearch Journal of Life Science2355-99262017-08-014211912810.21776/ub.rjls.2017.004.02.586Antioxidant Activity of Purified Active Peptide Derived from Spirulina platensis Enzymatic HydrolysatesNur Maulida Safitri0Endang Yuli Herawati1Jue Liang Hsu2Brawijaya UniversityBrawijaya UniversityNational Pingtung University of Science and TechnologyThe aim of this study is to isolate the antioxidative peptide from Spirulina platensis. Peptide was obtained by proteolytic digestion, ultrafiltration, fractionation by RP-HPLC, identified by LC-MS/MS—MASCOT Distiller and measured its antioxidant activity by DPPH (2.2-Diphenyl-1-picrylhydrazyl) assay. Results showed that thermolysin was the most effective enzyme to digest this algae. The active peptide Phe-Ser-Glu-Ser-Ser-Ala-Pro-Glu-Gln-His-Tyr (m/z 1281.51) was identified and synthetized, which exhibited 45.98 ± 1.7% at concentration 128.15 µg/mL. Therefore, S. platensis is indicated as a potential therapeutic source for combating oxidative stress.http://rjls.ub.ac.id/index.php/rjls/article/view/177AntioxidantCyanobacteriaDPPHLC-MSRP-HPLC |
| spellingShingle | Nur Maulida Safitri Endang Yuli Herawati Jue Liang Hsu Antioxidant Activity of Purified Active Peptide Derived from Spirulina platensis Enzymatic Hydrolysates Research Journal of Life Science Antioxidant Cyanobacteria DPPH LC-MS RP-HPLC |
| title | Antioxidant Activity of Purified Active Peptide Derived from Spirulina platensis Enzymatic Hydrolysates |
| title_full | Antioxidant Activity of Purified Active Peptide Derived from Spirulina platensis Enzymatic Hydrolysates |
| title_fullStr | Antioxidant Activity of Purified Active Peptide Derived from Spirulina platensis Enzymatic Hydrolysates |
| title_full_unstemmed | Antioxidant Activity of Purified Active Peptide Derived from Spirulina platensis Enzymatic Hydrolysates |
| title_short | Antioxidant Activity of Purified Active Peptide Derived from Spirulina platensis Enzymatic Hydrolysates |
| title_sort | antioxidant activity of purified active peptide derived from spirulina platensis enzymatic hydrolysates |
| topic | Antioxidant Cyanobacteria DPPH LC-MS RP-HPLC |
| url | http://rjls.ub.ac.id/index.php/rjls/article/view/177 |
| work_keys_str_mv | AT nurmaulidasafitri antioxidantactivityofpurifiedactivepeptidederivedfromspirulinaplatensisenzymatichydrolysates AT endangyuliherawati antioxidantactivityofpurifiedactivepeptidederivedfromspirulinaplatensisenzymatichydrolysates AT juelianghsu antioxidantactivityofpurifiedactivepeptidederivedfromspirulinaplatensisenzymatichydrolysates |