Charge-Based Separation of Micro- and Nanoparticles
Deterministic Lateral Displacement (DLD) is a label-free particle sorting method that separates by size continuously and with high resolution. By combining DLD with electric fields (eDLD), we show separation of a variety of nano and micro-sized particles primarily by their zeta potential. Zeta poten...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | Micromachines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-666X/11/11/1014 |
| _version_ | 1797547498619797504 |
|---|---|
| author | Bao D. Ho Jason P. Beech Jonas O. Tegenfeldt |
| author_facet | Bao D. Ho Jason P. Beech Jonas O. Tegenfeldt |
| author_sort | Bao D. Ho |
| collection | DOAJ |
| description | Deterministic Lateral Displacement (DLD) is a label-free particle sorting method that separates by size continuously and with high resolution. By combining DLD with electric fields (eDLD), we show separation of a variety of nano and micro-sized particles primarily by their zeta potential. Zeta potential is an indicator of electrokinetic charge—the charge corresponding to the electric field at the shear plane—an important property of micro- and nanoparticles in colloidal or separation science. We also demonstrate proof of principle of separation of nanoscale liposomes of different lipid compositions, with strong relevance for biomedicine. We perform careful characterization of relevant experimental conditions necessary to obtain adequate sorting of different particle types. By choosing a combination of frequency and amplitude, sorting can be made sensitive to the particle subgroup of interest. The enhanced displacement effect due to electrokinetics is found to be significant at low frequency and for particles with high zeta potential. The effect appears to scale with the square of the voltage, suggesting that it is associated with either non-linear electrokinetics or dielectrophoresis (DEP). However, since we observe large changes in separation behavior over the frequency range at which DEP forces are expected to remain constant, DEP can be ruled out. |
| first_indexed | 2024-03-10T14:44:56Z |
| format | Article |
| id | doaj.art-8dfcd1ee2dc84353bdd19ab564b791b6 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T14:44:56Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-8dfcd1ee2dc84353bdd19ab564b791b62023-11-20T21:27:32ZengMDPI AGMicromachines2072-666X2020-11-011111101410.3390/mi11111014Charge-Based Separation of Micro- and NanoparticlesBao D. Ho0Jason P. Beech1Jonas O. Tegenfeldt2Division of Solid State Physics and NanoLund, Physics Department, Lund University, P.O. Box 118, 22100 Lund, SwedenDivision of Solid State Physics and NanoLund, Physics Department, Lund University, P.O. Box 118, 22100 Lund, SwedenDivision of Solid State Physics and NanoLund, Physics Department, Lund University, P.O. Box 118, 22100 Lund, SwedenDeterministic Lateral Displacement (DLD) is a label-free particle sorting method that separates by size continuously and with high resolution. By combining DLD with electric fields (eDLD), we show separation of a variety of nano and micro-sized particles primarily by their zeta potential. Zeta potential is an indicator of electrokinetic charge—the charge corresponding to the electric field at the shear plane—an important property of micro- and nanoparticles in colloidal or separation science. We also demonstrate proof of principle of separation of nanoscale liposomes of different lipid compositions, with strong relevance for biomedicine. We perform careful characterization of relevant experimental conditions necessary to obtain adequate sorting of different particle types. By choosing a combination of frequency and amplitude, sorting can be made sensitive to the particle subgroup of interest. The enhanced displacement effect due to electrokinetics is found to be significant at low frequency and for particles with high zeta potential. The effect appears to scale with the square of the voltage, suggesting that it is associated with either non-linear electrokinetics or dielectrophoresis (DEP). However, since we observe large changes in separation behavior over the frequency range at which DEP forces are expected to remain constant, DEP can be ruled out.https://www.mdpi.com/2072-666X/11/11/1014electrokinetic deterministic lateral displacementcharge-based separation |
| spellingShingle | Bao D. Ho Jason P. Beech Jonas O. Tegenfeldt Charge-Based Separation of Micro- and Nanoparticles Micromachines electrokinetic deterministic lateral displacement charge-based separation |
| title | Charge-Based Separation of Micro- and Nanoparticles |
| title_full | Charge-Based Separation of Micro- and Nanoparticles |
| title_fullStr | Charge-Based Separation of Micro- and Nanoparticles |
| title_full_unstemmed | Charge-Based Separation of Micro- and Nanoparticles |
| title_short | Charge-Based Separation of Micro- and Nanoparticles |
| title_sort | charge based separation of micro and nanoparticles |
| topic | electrokinetic deterministic lateral displacement charge-based separation |
| url | https://www.mdpi.com/2072-666X/11/11/1014 |
| work_keys_str_mv | AT baodho chargebasedseparationofmicroandnanoparticles AT jasonpbeech chargebasedseparationofmicroandnanoparticles AT jonasotegenfeldt chargebasedseparationofmicroandnanoparticles |