Natural variations in the non-coding region of ZmNAC080308 contributes maintaining grain yield under drought stress in maize
Abstract Background Natural variations derived from both evolutionary selection and genetic recombination, presume to have important functions to respond to various abiotic stresses, which could be used to improve drought tolerance via genomic selection. Results In the present study, the NAC-encodin...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-06-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-021-03072-9 |
| _version_ | 1818456539110834176 |
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| author | Nan Wang Ming Cheng Yong Chen Bojuan Liu Xiaonan Wang Guojun Li Yueheng Zhou Ping Luo Zhangying Xi Hongjun Yong Degui Zhang Mingshun Li Xuecai Zhang Felix San Vicente Zhuanfang Hao Xinhai Li |
| author_facet | Nan Wang Ming Cheng Yong Chen Bojuan Liu Xiaonan Wang Guojun Li Yueheng Zhou Ping Luo Zhangying Xi Hongjun Yong Degui Zhang Mingshun Li Xuecai Zhang Felix San Vicente Zhuanfang Hao Xinhai Li |
| author_sort | Nan Wang |
| collection | DOAJ |
| description | Abstract Background Natural variations derived from both evolutionary selection and genetic recombination, presume to have important functions to respond to various abiotic stresses, which could be used to improve drought tolerance via genomic selection. Results In the present study, the NAC-encoding gene of ZmNAC080308 was cloned and sequenced in 199 inbred lines in maize. Phylogenetic analysis showed that ZmNAC080308 is closely clusteredinto the same group with other well-known NAC genes responding to improve drought tolerance. In total, 86 SNPs and 47 InDels were identified in the generic region of ZmNAC080308, 19 of these variations were associated with GY (grain yield) in different environments. Nine variations in the 5’-UTR region of ZmNAC080308 are closely linked, they might regulate the gene expression and respond to improve GY under drought condition via Sp1-mediated transactivation. Two haplotypes (Hap1 and Hap2) identified in the, 5’-UTR region using the nine variations, and Hap2 containing insertion variants, exhibited 15.47 % higher GY under drought stress condition. Further, a functional marker was developed to predict the drought stress tolerance in a US maize inbred line panel. Lines carrying Hap2 exhibited > 10 % higher GY than those carrying Hap1 under drought stress condition. In Arabidopsis, overexpression ZmNAC080308 enhanced drought tolerance. Conclusions ZmNAC080308 is an important gene responding to drought tolerance, a functional marker is developed for improving maize drought tolerance by selecting this gene. |
| first_indexed | 2024-12-14T22:28:16Z |
| format | Article |
| id | doaj.art-8dfd9ef437e84598aa8718cf99e72312 |
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| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-14T22:28:16Z |
| publishDate | 2021-06-01 |
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| series | BMC Plant Biology |
| spelling | doaj.art-8dfd9ef437e84598aa8718cf99e723122022-12-21T22:45:17ZengBMCBMC Plant Biology1471-22292021-06-0121111310.1186/s12870-021-03072-9Natural variations in the non-coding region of ZmNAC080308 contributes maintaining grain yield under drought stress in maizeNan Wang0Ming Cheng1Yong Chen2Bojuan Liu3Xiaonan Wang4Guojun Li5Yueheng Zhou6Ping Luo7Zhangying Xi8Hongjun Yong9Degui Zhang10Mingshun Li11Xuecai Zhang12Felix San Vicente13Zhuanfang Hao14Xinhai Li15Institute of Crop Sciences, Chinese Academy of Agricultural SciencesInstitute of Crop Sciences, Chinese Academy of Agricultural SciencesInstitute of Crop Sciences, Chinese Academy of Agricultural SciencesInstitute of Crop Sciences, Chinese Academy of Agricultural SciencesInstitute of Crop Sciences, Chinese Academy of Agricultural SciencesInstitute of Crop Sciences, Chinese Academy of Agricultural SciencesInstitute of Crop Sciences, Chinese Academy of Agricultural SciencesInstitute of Crop Sciences, Chinese Academy of Agricultural SciencesCollege of Agronomy, Henan Agricultural UniversityInstitute of Crop Sciences, Chinese Academy of Agricultural SciencesInstitute of Crop Sciences, Chinese Academy of Agricultural SciencesInstitute of Crop Sciences, Chinese Academy of Agricultural SciencesInternational Maize and Wheat Improvement Center (CIMMYT)International Maize and Wheat Improvement Center (CIMMYT)Institute of Crop Sciences, Chinese Academy of Agricultural SciencesInstitute of Crop Sciences, Chinese Academy of Agricultural SciencesAbstract Background Natural variations derived from both evolutionary selection and genetic recombination, presume to have important functions to respond to various abiotic stresses, which could be used to improve drought tolerance via genomic selection. Results In the present study, the NAC-encoding gene of ZmNAC080308 was cloned and sequenced in 199 inbred lines in maize. Phylogenetic analysis showed that ZmNAC080308 is closely clusteredinto the same group with other well-known NAC genes responding to improve drought tolerance. In total, 86 SNPs and 47 InDels were identified in the generic region of ZmNAC080308, 19 of these variations were associated with GY (grain yield) in different environments. Nine variations in the 5’-UTR region of ZmNAC080308 are closely linked, they might regulate the gene expression and respond to improve GY under drought condition via Sp1-mediated transactivation. Two haplotypes (Hap1 and Hap2) identified in the, 5’-UTR region using the nine variations, and Hap2 containing insertion variants, exhibited 15.47 % higher GY under drought stress condition. Further, a functional marker was developed to predict the drought stress tolerance in a US maize inbred line panel. Lines carrying Hap2 exhibited > 10 % higher GY than those carrying Hap1 under drought stress condition. In Arabidopsis, overexpression ZmNAC080308 enhanced drought tolerance. Conclusions ZmNAC080308 is an important gene responding to drought tolerance, a functional marker is developed for improving maize drought tolerance by selecting this gene.https://doi.org/10.1186/s12870-021-03072-9Maize (Zea Mays L.)NAC transcription factorNatural variationsNon-coding regionDrought tolerance |
| spellingShingle | Nan Wang Ming Cheng Yong Chen Bojuan Liu Xiaonan Wang Guojun Li Yueheng Zhou Ping Luo Zhangying Xi Hongjun Yong Degui Zhang Mingshun Li Xuecai Zhang Felix San Vicente Zhuanfang Hao Xinhai Li Natural variations in the non-coding region of ZmNAC080308 contributes maintaining grain yield under drought stress in maize BMC Plant Biology Maize (Zea Mays L.) NAC transcription factor Natural variations Non-coding region Drought tolerance |
| title | Natural variations in the non-coding region of ZmNAC080308 contributes maintaining grain yield under drought stress in maize |
| title_full | Natural variations in the non-coding region of ZmNAC080308 contributes maintaining grain yield under drought stress in maize |
| title_fullStr | Natural variations in the non-coding region of ZmNAC080308 contributes maintaining grain yield under drought stress in maize |
| title_full_unstemmed | Natural variations in the non-coding region of ZmNAC080308 contributes maintaining grain yield under drought stress in maize |
| title_short | Natural variations in the non-coding region of ZmNAC080308 contributes maintaining grain yield under drought stress in maize |
| title_sort | natural variations in the non coding region of zmnac080308 contributes maintaining grain yield under drought stress in maize |
| topic | Maize (Zea Mays L.) NAC transcription factor Natural variations Non-coding region Drought tolerance |
| url | https://doi.org/10.1186/s12870-021-03072-9 |
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