Expression of nonmuscle myosin IIC is regulated by non-canonical binding activity of miRNAs
Summary: The expression of mechanoresponsive nonmuscle myosin II (NMII)C is found to be inducible during tumor progression, but its mechanism is yet to be explored. Here, we report a group of microRNAs (mmu-miR-200a-5p, mmu-miR-532-3p, mmu-miR-680, and mmu-miR-1901) can significantly repress the exp...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223024616 |
| _version_ | 1797388752210886656 |
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| author | Kumarjeet Banerjee Shekhar Saha Shaoli Das Suman Ghosal Indranil Ghosh Abhimanyu Basu Siddhartha S. Jana |
| author_facet | Kumarjeet Banerjee Shekhar Saha Shaoli Das Suman Ghosal Indranil Ghosh Abhimanyu Basu Siddhartha S. Jana |
| author_sort | Kumarjeet Banerjee |
| collection | DOAJ |
| description | Summary: The expression of mechanoresponsive nonmuscle myosin II (NMII)C is found to be inducible during tumor progression, but its mechanism is yet to be explored. Here, we report a group of microRNAs (mmu-miR-200a-5p, mmu-miR-532-3p, mmu-miR-680, and mmu-miR-1901) can significantly repress the expression of nonmuscle myosin IIC (NMIIC). Interestingly, these microRNAs have both canonical and non-canonical binding sites at 3/UTR and coding sequence (CDS) of NMIIC’s heavy chain (HC) mRNA. Each of the miRNA downregulates NMHC-IIC to a different degree as assessed by dual-luciferase and immunoblot analyses. When we abolish the complementary base pairing at canonical binding site, mmu-miR-532-3p can still bind at non-canonical binding site and form Argonaute2 (AGO2)-miRNA complex to downregulate the expression of NMIIC. Modulating the expression of NMIIC by miR-532-3p in mouse mammary tumor cells, 4T1, increases its tumorigenic potential both in vitro and in vivo. Together, these studies provide the functional role of miRNA’s non-canonical binding mediated NMIIC regulation in tumor cells. |
| first_indexed | 2024-03-08T22:45:22Z |
| format | Article |
| id | doaj.art-8dfe9c399c8d463fbb8584fcf450d186 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-08T22:45:22Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-8dfe9c399c8d463fbb8584fcf450d1862023-12-17T06:40:34ZengElsevieriScience2589-00422023-12-012612108384Expression of nonmuscle myosin IIC is regulated by non-canonical binding activity of miRNAsKumarjeet Banerjee0Shekhar Saha1Shaoli Das2Suman Ghosal3Indranil Ghosh4Abhimanyu Basu5Siddhartha S. Jana6School of Biological Sciences, Indian Association for the Cultivation of Science, Kolkata, IndiaDepartment of Microbiology, Immunology, and Cancer Biology, Charlottesville, VA, USACenter for Cancer Research, National Cancer Institute, Bethesda, MD, USABioinformatics and Computational Biosciences Branch, National Institute of Allergy and Infectious Diseases, Bethesda, MD, USASchool of Biological Sciences, Indian Association for the Cultivation of Science, Kolkata, IndiaDepartment of General Surgery, Institute of Post Graduate Medical Education and Research, Kolkata, IndiaSchool of Biological Sciences, Indian Association for the Cultivation of Science, Kolkata, India; Corresponding authorSummary: The expression of mechanoresponsive nonmuscle myosin II (NMII)C is found to be inducible during tumor progression, but its mechanism is yet to be explored. Here, we report a group of microRNAs (mmu-miR-200a-5p, mmu-miR-532-3p, mmu-miR-680, and mmu-miR-1901) can significantly repress the expression of nonmuscle myosin IIC (NMIIC). Interestingly, these microRNAs have both canonical and non-canonical binding sites at 3/UTR and coding sequence (CDS) of NMIIC’s heavy chain (HC) mRNA. Each of the miRNA downregulates NMHC-IIC to a different degree as assessed by dual-luciferase and immunoblot analyses. When we abolish the complementary base pairing at canonical binding site, mmu-miR-532-3p can still bind at non-canonical binding site and form Argonaute2 (AGO2)-miRNA complex to downregulate the expression of NMIIC. Modulating the expression of NMIIC by miR-532-3p in mouse mammary tumor cells, 4T1, increases its tumorigenic potential both in vitro and in vivo. Together, these studies provide the functional role of miRNA’s non-canonical binding mediated NMIIC regulation in tumor cells.http://www.sciencedirect.com/science/article/pii/S2589004223024616BiochemistryMolecular mechanism of gene regulationCell biology |
| spellingShingle | Kumarjeet Banerjee Shekhar Saha Shaoli Das Suman Ghosal Indranil Ghosh Abhimanyu Basu Siddhartha S. Jana Expression of nonmuscle myosin IIC is regulated by non-canonical binding activity of miRNAs iScience Biochemistry Molecular mechanism of gene regulation Cell biology |
| title | Expression of nonmuscle myosin IIC is regulated by non-canonical binding activity of miRNAs |
| title_full | Expression of nonmuscle myosin IIC is regulated by non-canonical binding activity of miRNAs |
| title_fullStr | Expression of nonmuscle myosin IIC is regulated by non-canonical binding activity of miRNAs |
| title_full_unstemmed | Expression of nonmuscle myosin IIC is regulated by non-canonical binding activity of miRNAs |
| title_short | Expression of nonmuscle myosin IIC is regulated by non-canonical binding activity of miRNAs |
| title_sort | expression of nonmuscle myosin iic is regulated by non canonical binding activity of mirnas |
| topic | Biochemistry Molecular mechanism of gene regulation Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223024616 |
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