Evaluation of the Effectiveness of the Biopreparation in Combination with the Polymer γ-PGA for the Biodegradation of Petroleum Contaminants in Soil
Biodegradation is a method of effectively removing petroleum hydrocarbons from the natural environment. This research focuses on the biodegradation of aliphatic hydrocarbons, monoaromatic hydrocarbons such as benzene, toluene, ethylbenzene, and all three xylene isomers (BTEX) and polycyclic aromatic...
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MDPI AG
2022-01-01
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| Online Access: | https://www.mdpi.com/1996-1944/15/2/400 |
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| author | Katarzyna Wojtowicz Teresa Steliga Piotr Kapusta Joanna Brzeszcz Tomasz Skalski |
| author_facet | Katarzyna Wojtowicz Teresa Steliga Piotr Kapusta Joanna Brzeszcz Tomasz Skalski |
| author_sort | Katarzyna Wojtowicz |
| collection | DOAJ |
| description | Biodegradation is a method of effectively removing petroleum hydrocarbons from the natural environment. This research focuses on the biodegradation of aliphatic hydrocarbons, monoaromatic hydrocarbons such as benzene, toluene, ethylbenzene, and all three xylene isomers (BTEX) and polycyclic aromatic hydrocarbons (PAHs) as a result of soil inoculation with a biopreparation A1 based on autochthonous microorganisms and a biopreparation A1 with the addition of γ-PGA. The research used biopreparation A1 made of the following strains: <i>Dietzia</i> sp. <i>IN133</i>, <i>Gordonia</i> sp. <i>IN138</i> <i>Mycolicibacterium frederiksbergense</i> <i>IN53</i>, <i>Rhodococcus erythropolis</i> <i>IN119</i>, <i>Rhodococcus</i> sp. <i>IN136</i> and <i>Pseudomonas</i> sp. <i>IN132</i>. The experiments were carried out in laboratory conditions (microbiological tests, respirometric tests, and in semi-technical conditions (<i>ex-situ</i> prism method). The biodegradation efficiency was assessed on the basis of respirometric tests, chromatographic analyses and toxicological tests. As a result of inoculation of AB soil with the biopreparation A1 within 6 months, a reduction of total petroleum hydrocarbons (TPH) (66.03%), BTEX (80.08%) and PAHs (38.86%) was achieved and its toxicity was reduced. Inoculation of AB soil with the biopreparation A1 with the addition of γ-PGA reduced the concentration of TPH, BTEX and PAHs by 79.21%, 90.19%, and 51.18%, respectively, and reduced its toxicity. The conducted research has shown that the addition of γ-PGA affects the efficiency of the biodegradation process of petroleum pollutants, increasing the degree of TPH biodegradation by 13.18%, BTEX by 10.11% and PAHs by 12.32% compared to pure biopreparation A1. |
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| id | doaj.art-8e0ab91b5e544d84801edfd2f3201e58 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T01:04:37Z |
| publishDate | 2022-01-01 |
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| spelling | doaj.art-8e0ab91b5e544d84801edfd2f3201e582023-11-23T14:28:31ZengMDPI AGMaterials1996-19442022-01-0115240010.3390/ma15020400Evaluation of the Effectiveness of the Biopreparation in Combination with the Polymer γ-PGA for the Biodegradation of Petroleum Contaminants in SoilKatarzyna Wojtowicz0Teresa Steliga1Piotr Kapusta2Joanna Brzeszcz3Tomasz Skalski4Oil and Gas Institute—National Research Institute, Lubicz 25 A, 31-503 Krakow, PolandOil and Gas Institute—National Research Institute, Lubicz 25 A, 31-503 Krakow, PolandOil and Gas Institute—National Research Institute, Lubicz 25 A, 31-503 Krakow, PolandOil and Gas Institute—National Research Institute, Lubicz 25 A, 31-503 Krakow, PolandBiotechnology Centre, Silesian University of Technology, Krzywoustego 8, 44-100 Gliwice, PolandBiodegradation is a method of effectively removing petroleum hydrocarbons from the natural environment. This research focuses on the biodegradation of aliphatic hydrocarbons, monoaromatic hydrocarbons such as benzene, toluene, ethylbenzene, and all three xylene isomers (BTEX) and polycyclic aromatic hydrocarbons (PAHs) as a result of soil inoculation with a biopreparation A1 based on autochthonous microorganisms and a biopreparation A1 with the addition of γ-PGA. The research used biopreparation A1 made of the following strains: <i>Dietzia</i> sp. <i>IN133</i>, <i>Gordonia</i> sp. <i>IN138</i> <i>Mycolicibacterium frederiksbergense</i> <i>IN53</i>, <i>Rhodococcus erythropolis</i> <i>IN119</i>, <i>Rhodococcus</i> sp. <i>IN136</i> and <i>Pseudomonas</i> sp. <i>IN132</i>. The experiments were carried out in laboratory conditions (microbiological tests, respirometric tests, and in semi-technical conditions (<i>ex-situ</i> prism method). The biodegradation efficiency was assessed on the basis of respirometric tests, chromatographic analyses and toxicological tests. As a result of inoculation of AB soil with the biopreparation A1 within 6 months, a reduction of total petroleum hydrocarbons (TPH) (66.03%), BTEX (80.08%) and PAHs (38.86%) was achieved and its toxicity was reduced. Inoculation of AB soil with the biopreparation A1 with the addition of γ-PGA reduced the concentration of TPH, BTEX and PAHs by 79.21%, 90.19%, and 51.18%, respectively, and reduced its toxicity. The conducted research has shown that the addition of γ-PGA affects the efficiency of the biodegradation process of petroleum pollutants, increasing the degree of TPH biodegradation by 13.18%, BTEX by 10.11% and PAHs by 12.32% compared to pure biopreparation A1.https://www.mdpi.com/1996-1944/15/2/400biodegradationpetroleum contaminantsbiopreparationinoculationpolyglutamic acidecotoxicology |
| spellingShingle | Katarzyna Wojtowicz Teresa Steliga Piotr Kapusta Joanna Brzeszcz Tomasz Skalski Evaluation of the Effectiveness of the Biopreparation in Combination with the Polymer γ-PGA for the Biodegradation of Petroleum Contaminants in Soil Materials biodegradation petroleum contaminants biopreparation inoculation polyglutamic acid ecotoxicology |
| title | Evaluation of the Effectiveness of the Biopreparation in Combination with the Polymer γ-PGA for the Biodegradation of Petroleum Contaminants in Soil |
| title_full | Evaluation of the Effectiveness of the Biopreparation in Combination with the Polymer γ-PGA for the Biodegradation of Petroleum Contaminants in Soil |
| title_fullStr | Evaluation of the Effectiveness of the Biopreparation in Combination with the Polymer γ-PGA for the Biodegradation of Petroleum Contaminants in Soil |
| title_full_unstemmed | Evaluation of the Effectiveness of the Biopreparation in Combination with the Polymer γ-PGA for the Biodegradation of Petroleum Contaminants in Soil |
| title_short | Evaluation of the Effectiveness of the Biopreparation in Combination with the Polymer γ-PGA for the Biodegradation of Petroleum Contaminants in Soil |
| title_sort | evaluation of the effectiveness of the biopreparation in combination with the polymer γ pga for the biodegradation of petroleum contaminants in soil |
| topic | biodegradation petroleum contaminants biopreparation inoculation polyglutamic acid ecotoxicology |
| url | https://www.mdpi.com/1996-1944/15/2/400 |
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