Genistein Up-Regulates the Expression of EGF and E-Cadherin in the Treatment of Senile Vaginitis
Investigating the therapeutic effect of genistein (Gen) on postmenopausal senile vaginitis (SV) and its mechanism of action. Adult SPF female Wistar rats were selected to establish a bilateral ovariectomized animal model (OVX), which simulated senile vaginitis dominated by estrogen deficiency in ova...
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MDPI AG
2022-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/8/2388 |
| _version_ | 1797434370666004480 |
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| author | Yarui Sun Lei Wang Bo Wang Yanli Meng Weiming Wang |
| author_facet | Yarui Sun Lei Wang Bo Wang Yanli Meng Weiming Wang |
| author_sort | Yarui Sun |
| collection | DOAJ |
| description | Investigating the therapeutic effect of genistein (Gen) on postmenopausal senile vaginitis (SV) and its mechanism of action. Adult SPF female Wistar rats were selected to establish a bilateral ovariectomized animal model (OVX), which simulated senile vaginitis dominated by estrogen deficiency in ovarian dysfunction. After 14 days of continuous treatment, the morphology of vaginal epithelial tissue was observed and various types of epithelial cells were counted, and the body mass and uterine and vaginal index of rats were measured. the levels of vaginal tissue secretion, microorganism, hormone and glycogen in each group were measured and the reproductive health was evaluated clinically. The protein expression and mRNA expression of epidermal growth factor (EGF) and E-cadherin (E-cadherin) in vaginal tissues were detected by immunohistochemistry and RT-PCR, respectively. Result showed that Genistein lowered vaginal pH, increased vaginal index and vaginal health score, thickened epithelial layers and improved vaginal tissue atrophy after administration. Genistein also increased the contents of glycogen and Lactobacillus in vagina, and promoted the expression of EGF, E-cadherin protein and mRNA. To sum up, there is no significant change in serum E2 and FSH levels, indicating that genistein has no effect on hormone levels in rats. genistein promoted the proliferation of vaginal epithelial cells, thickened epithelial layers and the vaginal wall, which improved the resistance of vaginal epithelium, the recovery of self-cleaning ability and healed the vaginal wound and erosive surface to improve atrophy. |
| first_indexed | 2024-03-09T10:31:16Z |
| format | Article |
| id | doaj.art-8e0bd680792f4faeb18174f607c9cc92 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T10:31:16Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-8e0bd680792f4faeb18174f607c9cc922023-12-01T21:15:44ZengMDPI AGMolecules1420-30492022-04-01278238810.3390/molecules27082388Genistein Up-Regulates the Expression of EGF and E-Cadherin in the Treatment of Senile VaginitisYarui Sun0Lei Wang1Bo Wang2Yanli Meng3Weiming Wang4Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150040, ChinaHeilongjiang Academy of Chinese Medicine Sciences, Harbin 150040, ChinaHeilongjiang Academy of Chinese Medicine Sciences, Harbin 150040, ChinaHeilongjiang Academy of Chinese Medicine Sciences, Harbin 150040, ChinaHeilongjiang Academy of Chinese Medicine Sciences, Harbin 150040, ChinaInvestigating the therapeutic effect of genistein (Gen) on postmenopausal senile vaginitis (SV) and its mechanism of action. Adult SPF female Wistar rats were selected to establish a bilateral ovariectomized animal model (OVX), which simulated senile vaginitis dominated by estrogen deficiency in ovarian dysfunction. After 14 days of continuous treatment, the morphology of vaginal epithelial tissue was observed and various types of epithelial cells were counted, and the body mass and uterine and vaginal index of rats were measured. the levels of vaginal tissue secretion, microorganism, hormone and glycogen in each group were measured and the reproductive health was evaluated clinically. The protein expression and mRNA expression of epidermal growth factor (EGF) and E-cadherin (E-cadherin) in vaginal tissues were detected by immunohistochemistry and RT-PCR, respectively. Result showed that Genistein lowered vaginal pH, increased vaginal index and vaginal health score, thickened epithelial layers and improved vaginal tissue atrophy after administration. Genistein also increased the contents of glycogen and Lactobacillus in vagina, and promoted the expression of EGF, E-cadherin protein and mRNA. To sum up, there is no significant change in serum E2 and FSH levels, indicating that genistein has no effect on hormone levels in rats. genistein promoted the proliferation of vaginal epithelial cells, thickened epithelial layers and the vaginal wall, which improved the resistance of vaginal epithelium, the recovery of self-cleaning ability and healed the vaginal wound and erosive surface to improve atrophy.https://www.mdpi.com/1420-3049/27/8/2388genisteinsenile vaginitisovariectomyepidermal growth factorE-cadherin |
| spellingShingle | Yarui Sun Lei Wang Bo Wang Yanli Meng Weiming Wang Genistein Up-Regulates the Expression of EGF and E-Cadherin in the Treatment of Senile Vaginitis Molecules genistein senile vaginitis ovariectomy epidermal growth factor E-cadherin |
| title | Genistein Up-Regulates the Expression of EGF and E-Cadherin in the Treatment of Senile Vaginitis |
| title_full | Genistein Up-Regulates the Expression of EGF and E-Cadherin in the Treatment of Senile Vaginitis |
| title_fullStr | Genistein Up-Regulates the Expression of EGF and E-Cadherin in the Treatment of Senile Vaginitis |
| title_full_unstemmed | Genistein Up-Regulates the Expression of EGF and E-Cadherin in the Treatment of Senile Vaginitis |
| title_short | Genistein Up-Regulates the Expression of EGF and E-Cadherin in the Treatment of Senile Vaginitis |
| title_sort | genistein up regulates the expression of egf and e cadherin in the treatment of senile vaginitis |
| topic | genistein senile vaginitis ovariectomy epidermal growth factor E-cadherin |
| url | https://www.mdpi.com/1420-3049/27/8/2388 |
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