Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties
The aim of this research was phenolics and protein characterization and antioxidant properties evaluation of skimmed thermally treated goat’s milk powder enriched with different concentration of grape pomace seed extract (SE). The dominant phenolics in SE were phenolic acids, flavan–3-ols and procya...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/7/965 |
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| author | Danijel D. Milinčić Aleksandar Ž. Kostić Uroš M. Gašić Steva Lević Slađana P. Stanojević Miroljub B. Barać Živoslav Lj. Tešić Viktor Nedović Mirjana B. Pešić |
| author_facet | Danijel D. Milinčić Aleksandar Ž. Kostić Uroš M. Gašić Steva Lević Slađana P. Stanojević Miroljub B. Barać Živoslav Lj. Tešić Viktor Nedović Mirjana B. Pešić |
| author_sort | Danijel D. Milinčić |
| collection | DOAJ |
| description | The aim of this research was phenolics and protein characterization and antioxidant properties evaluation of skimmed thermally treated goat’s milk powder enriched with different concentration of grape pomace seed extract (SE). The dominant phenolics in SE were phenolic acids, flavan–3-ols and procyanidins. Different electrophoretic techniques together with UHPLC-MS/MS analysis revealed the presence of phenolics-protein interactions in the samples, mainly procyanidins with whey protein/caseins complexes. Addition of SE into thermally treated goat’s milk significantly improved antioxidant properties of goat’s milk such as TAC, FRP, DPPH<sup>•</sup> and ABTS<sup>•+</sup> scavenging activity. Gallic acid, catechin, and procyanidins mostly contributed to these activities. The schematic representation of phenolics–casein micelles interactions in thermally treated goat’s milk enriched with SE was given. The addition of SE into thermally treated goat’s milk can be a promising strategy in food waste recovery and to enhance the beneficial health effects of goat’s milk-based functional foods. |
| first_indexed | 2024-03-09T04:45:07Z |
| format | Article |
| id | doaj.art-8e0d9933081b4ba48fc7c1accb91eba4 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T04:45:07Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-8e0d9933081b4ba48fc7c1accb91eba42023-12-03T13:16:57ZengMDPI AGBiomolecules2218-273X2021-06-0111796510.3390/biom11070965Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant PropertiesDanijel D. Milinčić0Aleksandar Ž. Kostić1Uroš M. Gašić2Steva Lević3Slađana P. Stanojević4Miroljub B. Barać5Živoslav Lj. Tešić6Viktor Nedović7Mirjana B. Pešić8Faculty of Agriculture, Institute of Food Technology and Biochemistry, University of Belgrade, Nemanjina 6, 11080 Belgrade, SerbiaFaculty of Agriculture, Institute of Food Technology and Biochemistry, University of Belgrade, Nemanjina 6, 11080 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaFaculty of Agriculture, Institute of Food Technology and Biochemistry, University of Belgrade, Nemanjina 6, 11080 Belgrade, SerbiaFaculty of Agriculture, Institute of Food Technology and Biochemistry, University of Belgrade, Nemanjina 6, 11080 Belgrade, SerbiaFaculty of Agriculture, Institute of Food Technology and Biochemistry, University of Belgrade, Nemanjina 6, 11080 Belgrade, SerbiaChair of Analytical Chemistry, Faculty of Chemistry, University of Belgrade, Studentski Trg 12–16, 11000 Belgrade, SerbiaFaculty of Agriculture, Institute of Food Technology and Biochemistry, University of Belgrade, Nemanjina 6, 11080 Belgrade, SerbiaFaculty of Agriculture, Institute of Food Technology and Biochemistry, University of Belgrade, Nemanjina 6, 11080 Belgrade, SerbiaThe aim of this research was phenolics and protein characterization and antioxidant properties evaluation of skimmed thermally treated goat’s milk powder enriched with different concentration of grape pomace seed extract (SE). The dominant phenolics in SE were phenolic acids, flavan–3-ols and procyanidins. Different electrophoretic techniques together with UHPLC-MS/MS analysis revealed the presence of phenolics-protein interactions in the samples, mainly procyanidins with whey protein/caseins complexes. Addition of SE into thermally treated goat’s milk significantly improved antioxidant properties of goat’s milk such as TAC, FRP, DPPH<sup>•</sup> and ABTS<sup>•+</sup> scavenging activity. Gallic acid, catechin, and procyanidins mostly contributed to these activities. The schematic representation of phenolics–casein micelles interactions in thermally treated goat’s milk enriched with SE was given. The addition of SE into thermally treated goat’s milk can be a promising strategy in food waste recovery and to enhance the beneficial health effects of goat’s milk-based functional foods.https://www.mdpi.com/2218-273X/11/7/965grape pomace seedskimmed goat’s milkphenolicsmilk proteinphenolic–protein interactions |
| spellingShingle | Danijel D. Milinčić Aleksandar Ž. Kostić Uroš M. Gašić Steva Lević Slađana P. Stanojević Miroljub B. Barać Živoslav Lj. Tešić Viktor Nedović Mirjana B. Pešić Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties Biomolecules grape pomace seed skimmed goat’s milk phenolics milk protein phenolic–protein interactions |
| title | Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties |
| title_full | Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties |
| title_fullStr | Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties |
| title_full_unstemmed | Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties |
| title_short | Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties |
| title_sort | skimmed goat s milk powder enriched with grape pomace seed extract phenolics and protein characterization and antioxidant properties |
| topic | grape pomace seed skimmed goat’s milk phenolics milk protein phenolic–protein interactions |
| url | https://www.mdpi.com/2218-273X/11/7/965 |
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