Serotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12.
In addition to its role as neurotransmitter, serotonin (5-HT) is an important modulator of inflammation and immunity. Here, we report novel findings suggesting a 5-HT involvement in T cell migration. In particular, we show that 5-HT tunes the responsiveness of human T lymphocytes to the broadly expr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21853036/?tool=EBI |
| _version_ | 1818402853700501504 |
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| author | Elena Magrini Ildikò Szabò Andrea Doni Javier Cibella Antonella Viola |
| author_facet | Elena Magrini Ildikò Szabò Andrea Doni Javier Cibella Antonella Viola |
| author_sort | Elena Magrini |
| collection | DOAJ |
| description | In addition to its role as neurotransmitter, serotonin (5-HT) is an important modulator of inflammation and immunity. Here, we report novel findings suggesting a 5-HT involvement in T cell migration. In particular, we show that 5-HT tunes the responsiveness of human T lymphocytes to the broadly expressed chemokine CXCL12 in transwell migration assays. By real-time PCR, western blot analysis and electrophysiological patch clamp experiments, we demonstrate that the type 3 5-HT receptor (5-HT(3)) is functionally expressed in human primary T cells. In addition, specific 5-HT(3) receptor agonists selectively decrease T cell migration towards gradients of CXCL12 but not of inflammatory chemokines, such as CCL2 and CCL5. In transmigration experiments, 5-HT(3) receptor stimulation reverts the inhibitory effect of endothelial-bound CXCL12 on T cell migration. Our data suggest that the reduced T cell responsiveness to CXCL12 induced by 5-HT may occur to facilitate T cell extravasation and migration into inflamed tissues. |
| first_indexed | 2024-12-14T08:14:58Z |
| format | Article |
| id | doaj.art-8e0db73ea87843669a8800029f03fce7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T08:14:58Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8e0db73ea87843669a8800029f03fce72022-12-21T23:09:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0168e2248210.1371/journal.pone.0022482Serotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12.Elena MagriniIldikò SzabòAndrea DoniJavier CibellaAntonella ViolaIn addition to its role as neurotransmitter, serotonin (5-HT) is an important modulator of inflammation and immunity. Here, we report novel findings suggesting a 5-HT involvement in T cell migration. In particular, we show that 5-HT tunes the responsiveness of human T lymphocytes to the broadly expressed chemokine CXCL12 in transwell migration assays. By real-time PCR, western blot analysis and electrophysiological patch clamp experiments, we demonstrate that the type 3 5-HT receptor (5-HT(3)) is functionally expressed in human primary T cells. In addition, specific 5-HT(3) receptor agonists selectively decrease T cell migration towards gradients of CXCL12 but not of inflammatory chemokines, such as CCL2 and CCL5. In transmigration experiments, 5-HT(3) receptor stimulation reverts the inhibitory effect of endothelial-bound CXCL12 on T cell migration. Our data suggest that the reduced T cell responsiveness to CXCL12 induced by 5-HT may occur to facilitate T cell extravasation and migration into inflamed tissues.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21853036/?tool=EBI |
| spellingShingle | Elena Magrini Ildikò Szabò Andrea Doni Javier Cibella Antonella Viola Serotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12. PLoS ONE |
| title | Serotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12. |
| title_full | Serotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12. |
| title_fullStr | Serotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12. |
| title_full_unstemmed | Serotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12. |
| title_short | Serotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12. |
| title_sort | serotonin mediated tuning of human helper t cell responsiveness to the chemokine cxcl12 |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21853036/?tool=EBI |
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