The pseudogene-derived long noncoding RNA SFTA1P is down-regulated and suppresses cell migration and invasion in lung adenocarcinoma
Pseudogenes were once considered to be genomic fossils without biological function. Interestingly, recent evidence showed that a lot of pseudogenes are transcribed in human cancers, and their alterations contribute to multiple cancer development and progression. It is apparent that many pseudogenes...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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IOS Press
2017-02-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317691418 |
| _version_ | 1818878375603732480 |
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| author | Hua Zhang Yaqiong Xiong Rui Xia Chenchen Wei Xuefei Shi Fengqi Nie |
| author_facet | Hua Zhang Yaqiong Xiong Rui Xia Chenchen Wei Xuefei Shi Fengqi Nie |
| author_sort | Hua Zhang |
| collection | DOAJ |
| description | Pseudogenes were once considered to be genomic fossils without biological function. Interestingly, recent evidence showed that a lot of pseudogenes are transcribed in human cancers, and their alterations contribute to multiple cancer development and progression. It is apparent that many pseudogenes transcribe noncoding RNAs and contribute to the role noncoding genome plays in human cancers. On this basis, some pseudogene transcripts are currently ranked among the classes of long noncoding RNAs. In this study, we identified a new pseudogene-derived long noncoding RNA termed SFTA1P by analyzing the microarray data of non–small cell lung cancer from Gene Expression Omnibus datasets. We found that SFTA1P expression was significantly decreased in non–small cell lung cancer tissues compared with normal tissues in non–small cell lung cancer microarray data. Moreover, decreased SFTA1P expression is only correlated with lung adenocarcinoma patients’ poor survival time but not with lung squamous cell carcinoma patients’ survival. In addition, gain-of-function studies including growth curves, migration, invasion assays, and in vivo studies were performed to verify the tumor suppressor role of SFTA1P in non–small cell lung cancer. Finally, the potential underlying pathways involved in SFTA1P were investigated by analyzing the SFTA1P-correlated genes in The Cancer Genome Atlas lung adenocarcinoma and normal tissues RNA sequencing data. Taken together, these findings demonstrate that pseudogene-derived long noncoding RNA SFTA1P exerts the tumor suppressor functions in human lung adenocarcinoma. Our investigation reveals the novel roles of pseudogene in lung adenocarcinoma, which may serve as a new target for lung adenocarcinoma diagnosis and therapy. |
| first_indexed | 2024-12-19T14:13:11Z |
| format | Article |
| id | doaj.art-8e1259d8ef2444ec89c0b1da3ba41a4a |
| institution | Directory Open Access Journal |
| issn | 1423-0380 |
| language | English |
| last_indexed | 2024-12-19T14:13:11Z |
| publishDate | 2017-02-01 |
| publisher | IOS Press |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj.art-8e1259d8ef2444ec89c0b1da3ba41a4a2022-12-21T20:18:04ZengIOS PressTumor Biology1423-03802017-02-013910.1177/1010428317691418The pseudogene-derived long noncoding RNA SFTA1P is down-regulated and suppresses cell migration and invasion in lung adenocarcinomaHua Zhang0Yaqiong Xiong1Rui Xia2Chenchen Wei3Xuefei Shi4Fengqi Nie5Department of Joint Trauma, Junan County People’s Hospital, Linyi, People’s Republic of ChinaDepartment of Respiratory Medicine, Huai’an First People’s Hospital, Nanjing Medical University, Huai’an, People’s Republic of ChinaDepartment of Clinical Laboratory, Nanjing Chest Hospital, Nanjing, People’s Republic of ChinaDepartment of Oncology, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, People’s Republic of ChinaDepartment of Respiratory Medicine, Huzhou Central Hospital, Huzhou, People’s Republic of ChinaDepartment of Oncology, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, People’s Republic of ChinaPseudogenes were once considered to be genomic fossils without biological function. Interestingly, recent evidence showed that a lot of pseudogenes are transcribed in human cancers, and their alterations contribute to multiple cancer development and progression. It is apparent that many pseudogenes transcribe noncoding RNAs and contribute to the role noncoding genome plays in human cancers. On this basis, some pseudogene transcripts are currently ranked among the classes of long noncoding RNAs. In this study, we identified a new pseudogene-derived long noncoding RNA termed SFTA1P by analyzing the microarray data of non–small cell lung cancer from Gene Expression Omnibus datasets. We found that SFTA1P expression was significantly decreased in non–small cell lung cancer tissues compared with normal tissues in non–small cell lung cancer microarray data. Moreover, decreased SFTA1P expression is only correlated with lung adenocarcinoma patients’ poor survival time but not with lung squamous cell carcinoma patients’ survival. In addition, gain-of-function studies including growth curves, migration, invasion assays, and in vivo studies were performed to verify the tumor suppressor role of SFTA1P in non–small cell lung cancer. Finally, the potential underlying pathways involved in SFTA1P were investigated by analyzing the SFTA1P-correlated genes in The Cancer Genome Atlas lung adenocarcinoma and normal tissues RNA sequencing data. Taken together, these findings demonstrate that pseudogene-derived long noncoding RNA SFTA1P exerts the tumor suppressor functions in human lung adenocarcinoma. Our investigation reveals the novel roles of pseudogene in lung adenocarcinoma, which may serve as a new target for lung adenocarcinoma diagnosis and therapy.https://doi.org/10.1177/1010428317691418 |
| spellingShingle | Hua Zhang Yaqiong Xiong Rui Xia Chenchen Wei Xuefei Shi Fengqi Nie The pseudogene-derived long noncoding RNA SFTA1P is down-regulated and suppresses cell migration and invasion in lung adenocarcinoma Tumor Biology |
| title | The pseudogene-derived long noncoding RNA SFTA1P is down-regulated and suppresses cell migration and invasion in lung adenocarcinoma |
| title_full | The pseudogene-derived long noncoding RNA SFTA1P is down-regulated and suppresses cell migration and invasion in lung adenocarcinoma |
| title_fullStr | The pseudogene-derived long noncoding RNA SFTA1P is down-regulated and suppresses cell migration and invasion in lung adenocarcinoma |
| title_full_unstemmed | The pseudogene-derived long noncoding RNA SFTA1P is down-regulated and suppresses cell migration and invasion in lung adenocarcinoma |
| title_short | The pseudogene-derived long noncoding RNA SFTA1P is down-regulated and suppresses cell migration and invasion in lung adenocarcinoma |
| title_sort | pseudogene derived long noncoding rna sfta1p is down regulated and suppresses cell migration and invasion in lung adenocarcinoma |
| url | https://doi.org/10.1177/1010428317691418 |
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