Bovine-Derived Xenografts Immobilized With Cryopreserved Stem Cells From Human Adipose and Dental Pulp Tissues Promote Bone Regeneration: A Radiographic and Histological Study
Adipose tissue-derived stem cells (ADSCs) and dental pulp stem cells (DPSCs) have become promising sources for bone tissue engineering. Our study aimed at evaluating bone regeneration potential of cryopreserved ADSCs and DPSCs combined with bovine-derived xenografts with 10% porcine collagen. In vit...
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.646690/full |
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| author | Yu Zhu Yu Zhu Yu Zhu Shi-min Wei Shi-min Wei Shi-min Wei Kai-xiao Yan Kai-xiao Yan Kai-xiao Yan Ying-xin Gu Ying-xin Gu Ying-xin Gu Hong-chang Lai Hong-chang Lai Hong-chang Lai Shi-chong Qiao Shi-chong Qiao Shi-chong Qiao |
| author_facet | Yu Zhu Yu Zhu Yu Zhu Shi-min Wei Shi-min Wei Shi-min Wei Kai-xiao Yan Kai-xiao Yan Kai-xiao Yan Ying-xin Gu Ying-xin Gu Ying-xin Gu Hong-chang Lai Hong-chang Lai Hong-chang Lai Shi-chong Qiao Shi-chong Qiao Shi-chong Qiao |
| author_sort | Yu Zhu |
| collection | DOAJ |
| description | Adipose tissue-derived stem cells (ADSCs) and dental pulp stem cells (DPSCs) have become promising sources for bone tissue engineering. Our study aimed at evaluating bone regeneration potential of cryopreserved ADSCs and DPSCs combined with bovine-derived xenografts with 10% porcine collagen. In vitro studies revealed that although DPSCs had higher proliferative abilities, ADSCs exhibited greater mineral depositions and higher osteogenic-related gene expression, indicating better osteogenic differentiation potential of ADSCs. After applying cryopreserved ADSCs and DPSCs in a critical-sized calvarial defect model, both cryopreserved mesenchymal stem cells significantly improved bone volume density and new bone area at 2, 4, and 8 weeks. Furthermore, the combined treatment with ADSCs and xenografts was more efficient in enhancing bone repair processes compared to combined treatment with DPCSs at all-time points. We also evaluated the sequential early bone healing process both histologically and radiographically, confirming a high agreement between these two methods. Based on these results, we propose grafting of the tissue-engineered construct seeded with cryopreserved ADSCs as a useful strategy in accelerating bone healing processes. |
| first_indexed | 2024-12-24T00:20:05Z |
| format | Article |
| id | doaj.art-8e12ac36d64c4d259f11d536d168cd10 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-24T00:20:05Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-8e12ac36d64c4d259f11d536d168cd102022-12-21T17:24:37ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-04-01910.3389/fbioe.2021.646690646690Bovine-Derived Xenografts Immobilized With Cryopreserved Stem Cells From Human Adipose and Dental Pulp Tissues Promote Bone Regeneration: A Radiographic and Histological StudyYu Zhu0Yu Zhu1Yu Zhu2Shi-min Wei3Shi-min Wei4Shi-min Wei5Kai-xiao Yan6Kai-xiao Yan7Kai-xiao Yan8Ying-xin Gu9Ying-xin Gu10Ying-xin Gu11Hong-chang Lai12Hong-chang Lai13Hong-chang Lai14Shi-chong Qiao15Shi-chong Qiao16Shi-chong Qiao17Department of Implant Dentistry, Shanghai Ninth People’s Hospital, College of Stomatology, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaNational Clinical Research Center for Oral Diseases, Shanghai, ChinaShanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Implant Dentistry, Shanghai Ninth People’s Hospital, College of Stomatology, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaNational Clinical Research Center for Oral Diseases, Shanghai, ChinaShanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Implant Dentistry, Shanghai Ninth People’s Hospital, College of Stomatology, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaNational Clinical Research Center for Oral Diseases, Shanghai, ChinaShanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Implant Dentistry, Shanghai Ninth People’s Hospital, College of Stomatology, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaNational Clinical Research Center for Oral Diseases, Shanghai, ChinaShanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Implant Dentistry, Shanghai Ninth People’s Hospital, College of Stomatology, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaNational Clinical Research Center for Oral Diseases, Shanghai, ChinaShanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Implant Dentistry, Shanghai Ninth People’s Hospital, College of Stomatology, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaNational Clinical Research Center for Oral Diseases, Shanghai, ChinaShanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University, Shanghai, ChinaAdipose tissue-derived stem cells (ADSCs) and dental pulp stem cells (DPSCs) have become promising sources for bone tissue engineering. Our study aimed at evaluating bone regeneration potential of cryopreserved ADSCs and DPSCs combined with bovine-derived xenografts with 10% porcine collagen. In vitro studies revealed that although DPSCs had higher proliferative abilities, ADSCs exhibited greater mineral depositions and higher osteogenic-related gene expression, indicating better osteogenic differentiation potential of ADSCs. After applying cryopreserved ADSCs and DPSCs in a critical-sized calvarial defect model, both cryopreserved mesenchymal stem cells significantly improved bone volume density and new bone area at 2, 4, and 8 weeks. Furthermore, the combined treatment with ADSCs and xenografts was more efficient in enhancing bone repair processes compared to combined treatment with DPCSs at all-time points. We also evaluated the sequential early bone healing process both histologically and radiographically, confirming a high agreement between these two methods. Based on these results, we propose grafting of the tissue-engineered construct seeded with cryopreserved ADSCs as a useful strategy in accelerating bone healing processes.https://www.frontiersin.org/articles/10.3389/fbioe.2021.646690/fulladipose tissue-derived stem cellsdental pulp stem cellsbone regenerationcalvarial defectμCT examinations |
| spellingShingle | Yu Zhu Yu Zhu Yu Zhu Shi-min Wei Shi-min Wei Shi-min Wei Kai-xiao Yan Kai-xiao Yan Kai-xiao Yan Ying-xin Gu Ying-xin Gu Ying-xin Gu Hong-chang Lai Hong-chang Lai Hong-chang Lai Shi-chong Qiao Shi-chong Qiao Shi-chong Qiao Bovine-Derived Xenografts Immobilized With Cryopreserved Stem Cells From Human Adipose and Dental Pulp Tissues Promote Bone Regeneration: A Radiographic and Histological Study Frontiers in Bioengineering and Biotechnology adipose tissue-derived stem cells dental pulp stem cells bone regeneration calvarial defect μCT examinations |
| title | Bovine-Derived Xenografts Immobilized With Cryopreserved Stem Cells From Human Adipose and Dental Pulp Tissues Promote Bone Regeneration: A Radiographic and Histological Study |
| title_full | Bovine-Derived Xenografts Immobilized With Cryopreserved Stem Cells From Human Adipose and Dental Pulp Tissues Promote Bone Regeneration: A Radiographic and Histological Study |
| title_fullStr | Bovine-Derived Xenografts Immobilized With Cryopreserved Stem Cells From Human Adipose and Dental Pulp Tissues Promote Bone Regeneration: A Radiographic and Histological Study |
| title_full_unstemmed | Bovine-Derived Xenografts Immobilized With Cryopreserved Stem Cells From Human Adipose and Dental Pulp Tissues Promote Bone Regeneration: A Radiographic and Histological Study |
| title_short | Bovine-Derived Xenografts Immobilized With Cryopreserved Stem Cells From Human Adipose and Dental Pulp Tissues Promote Bone Regeneration: A Radiographic and Histological Study |
| title_sort | bovine derived xenografts immobilized with cryopreserved stem cells from human adipose and dental pulp tissues promote bone regeneration a radiographic and histological study |
| topic | adipose tissue-derived stem cells dental pulp stem cells bone regeneration calvarial defect μCT examinations |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.646690/full |
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