Identification of mitochondria-related action targets of quercetin in melanoma cells
Melanoma is a complex and genetically heterogeneous malignant tumor with high rates of mortality. Although current therapies provide a short-term clinical benefit, they are unable to cure the majority of patients with metastatic melanoma. Therefore, the investigation of pathological mechanisms and t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-10-01
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| Series: | Mitochondrial DNA. Part B. Resources |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/23802359.2023.2268775 |
| _version_ | 1797638444580601856 |
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| author | Qing Xi Li Li Yongjie Yang Liubing Li Rongxin Zhang |
| author_facet | Qing Xi Li Li Yongjie Yang Liubing Li Rongxin Zhang |
| author_sort | Qing Xi |
| collection | DOAJ |
| description | Melanoma is a complex and genetically heterogeneous malignant tumor with high rates of mortality. Although current therapies provide a short-term clinical benefit, they are unable to cure the majority of patients with metastatic melanoma. Therefore, the investigation of pathological mechanisms and the development of new therapy strategies for melanoma are of great significance. Quercetin can effectively inhibit tumor growth in various tumors. However, the exact action mechanisms of quercetin against melanoma have not been comprehensively clarified, which limits its application. Accumulating evidence has suggested that the dysfunction of mitochondria is closely linked to carcinogenesis, and a better understanding of the regulation of mitochondria-related genes will shed light on providing new therapies for melanoma. In this study, we performed RNA-seq from melanoma B16-F1 cells treated with quercetin versus controls and screened for differentially expressed genes (DEGs). GO and KEGG enrichment analyses were performed, and a protein–protein interaction (PPI) network was constructed. Combining the results of RNA-seq, molecular docking, and bioinformatics analysis, we found six mitochondria-related genes, BTG2, CP, LRIG1, CYP1A1, GBP2, and MBNL1, which might be targets of quercetin in melanoma and provide an available targeting therapy strategy for melanoma. |
| first_indexed | 2024-03-11T13:03:36Z |
| format | Article |
| id | doaj.art-8e131ff0f6c7406181d55c0e2c298328 |
| institution | Directory Open Access Journal |
| issn | 2380-2359 |
| language | English |
| last_indexed | 2024-03-11T13:03:36Z |
| publishDate | 2023-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mitochondrial DNA. Part B. Resources |
| spelling | doaj.art-8e131ff0f6c7406181d55c0e2c2983282023-11-03T14:44:08ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592023-10-018101114111810.1080/23802359.2023.22687752268775Identification of mitochondria-related action targets of quercetin in melanoma cellsQing Xi0Li Li1Yongjie Yang2Liubing Li3Rongxin Zhang4Department of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical UniversityDepartment of Biotechnology, School of Life Sciences and Biopharmaceutics, Laboratory of Immunology and Inflammation, Guangdong Pharmaceutical UniversityDepartment of Biotechnology, School of Life Sciences and Biopharmaceutics, Laboratory of Immunology and Inflammation, Guangdong Pharmaceutical UniversityDepartment of Laboratory Medicine, The First Affiliated Hospital, Sun Yat-sen UniversityDepartment of Biotechnology, School of Life Sciences and Biopharmaceutics, Laboratory of Immunology and Inflammation, Guangdong Pharmaceutical UniversityMelanoma is a complex and genetically heterogeneous malignant tumor with high rates of mortality. Although current therapies provide a short-term clinical benefit, they are unable to cure the majority of patients with metastatic melanoma. Therefore, the investigation of pathological mechanisms and the development of new therapy strategies for melanoma are of great significance. Quercetin can effectively inhibit tumor growth in various tumors. However, the exact action mechanisms of quercetin against melanoma have not been comprehensively clarified, which limits its application. Accumulating evidence has suggested that the dysfunction of mitochondria is closely linked to carcinogenesis, and a better understanding of the regulation of mitochondria-related genes will shed light on providing new therapies for melanoma. In this study, we performed RNA-seq from melanoma B16-F1 cells treated with quercetin versus controls and screened for differentially expressed genes (DEGs). GO and KEGG enrichment analyses were performed, and a protein–protein interaction (PPI) network was constructed. Combining the results of RNA-seq, molecular docking, and bioinformatics analysis, we found six mitochondria-related genes, BTG2, CP, LRIG1, CYP1A1, GBP2, and MBNL1, which might be targets of quercetin in melanoma and provide an available targeting therapy strategy for melanoma.http://dx.doi.org/10.1080/23802359.2023.2268775melanomamitochondrial dysfunctionquercetinrna-seq |
| spellingShingle | Qing Xi Li Li Yongjie Yang Liubing Li Rongxin Zhang Identification of mitochondria-related action targets of quercetin in melanoma cells Mitochondrial DNA. Part B. Resources melanoma mitochondrial dysfunction quercetin rna-seq |
| title | Identification of mitochondria-related action targets of quercetin in melanoma cells |
| title_full | Identification of mitochondria-related action targets of quercetin in melanoma cells |
| title_fullStr | Identification of mitochondria-related action targets of quercetin in melanoma cells |
| title_full_unstemmed | Identification of mitochondria-related action targets of quercetin in melanoma cells |
| title_short | Identification of mitochondria-related action targets of quercetin in melanoma cells |
| title_sort | identification of mitochondria related action targets of quercetin in melanoma cells |
| topic | melanoma mitochondrial dysfunction quercetin rna-seq |
| url | http://dx.doi.org/10.1080/23802359.2023.2268775 |
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